As nanopartículas de quitosana foram obtidas pela técnica de gelificação iônica apresentando diâmetro de partícula variando entre 150 e 200 nm, com tamanho de partícula homogêneo. Foi observada uma alta eficiência de encapsulação dos nanossistemas para os venenos do escorpião T. serrulatus e para as serpentes B. jararaca e B. erythromelas. Essa eficiência foi comprovada pela dosagem das proteínas livres no sistema, pelo ensaio de retenção das proteínas ao polímero e ainda pelo infravermelho, que evidenciou as interações que ocorrem entre os grupamentos que compõem a quitosana e as proteínas que foram adicionadas ao sistema. Com a análise dos resultados obtidos no ensaio de liberação, foi possível concluir, que o sistema obtido trata-se de um sistema de liberação modificada, sendo bastante interessante sua utilização como adjuvante de imunização. Os títulos de anticorpos obtidos, após a imunização dos animais com as nanopartículas de quitosana, foram maiores ou iguais aos dos obtidos em animais imunizados com o adjuvante convencional. Estas características conferem às nanopartículas de quitosana a possibilidade de servir como base para a formulação de um novo imunoadjuvante, que irá auxiliar na obtenção de um novo soro mais eficaz, contra toxinas de venenos.
A obtenção, padronização e aplicação do nanossistemas como imunoadjuvante na produção de soros e vacinas contra venenos de animais peçonhentos proporcionou a produção de uma patente de inovação tecnológica, já depositada e dois artigos científicos.
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ANEXOS
- Patente depositada.
Sistemas nanoparticulados de quitosana aplicados como imunoadjuvante na produção de soros contra venenos de animais peçonhentos.