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[PDF] Top 20 Zika virus crosses an in vitro human blood brain barrier model

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Zika virus crosses an in vitro human blood brain barrier model

Zika virus crosses an in vitro human blood brain barrier model

... the virus by immunoluo- rescence (Fig. 2 a, b) and was detected in cell lysates by Western blotting after only a 2-h exposure ...expressed in i-BECs by Western blot analysis (Fig. 2 d) as well as ... Voir le document complet

10

Functional expression of a proton-coupled organic cation (H+/OC) antiporter in human brain capillary endothelial cell line hCMEC/D3, a human blood-brain barrier model.

Functional expression of a proton-coupled organic cation (H+/OC) antiporter in human brain capillary endothelial cell line hCMEC/D3, a human blood-brain barrier model.

... immortalized brain capillary endothelial cells (hCMEC/D3) have recently been developed as an in vitro human BBB model ...worldwide in pharmacological, toxicological, ... Voir le document complet

11

Antibody Neutralization of HIV-1 Crossing the Blood-Brain Barrier

Antibody Neutralization of HIV-1 Crossing the Blood-Brain Barrier

... the blood-brain ...established an in vitro model using the human brain microvascular endothelial cell line hCMEC/D3, which reproduces most characteristics of the ... Voir le document complet

12

Setting-up an in vitro model of rat blood-brain barrier (BBB): a focus on BBB impermeability and receptor-mediated transport

Setting-up an in vitro model of rat blood-brain barrier (BBB): a focus on BBB impermeability and receptor-mediated transport

... expression in isolated microvessels and low passage endothelial cell cultures from the same species, and between different species, most often from small animals such as rodents (mice and rats) or from cow and pig ... Voir le document complet

18

Prolonged Morphine Exposure Induces Increased Firm Adhesion in an in Vitro Model of the Blood-Brain Barrier.

Prolonged Morphine Exposure Induces Increased Firm Adhesion in an in Vitro Model of the Blood-Brain Barrier.

... Discussion In the studies presented here, the hCMEC/D3 cell line was used because it has been demonstrated to be stable in monoculture for several days after reaching confluence, allowing us to study the ... Voir le document complet

16

TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.

TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.

... TWEAK in brain pathology is fur- ther evidenced by data proving that TWEAK blocking antibodies or Fn14 decoy receptors are efficient in ani- mal models of ischemic stroke and brain edema ... Voir le document complet

15

A novel human induced pluripotent stem cell blood-brain barrier model: applicability to study antibody-triggered receptor-mediated transcytosis

A novel human induced pluripotent stem cell blood-brain barrier model: applicability to study antibody-triggered receptor-mediated transcytosis

... evaluated in a two-compartment in vitro Transwell BBB transport ...days in EM culture, the i-BECs were dissociated and seeded at a density of ...contacts in conluent cultures, in ... Voir le document complet

18

Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.

Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.

... specialization model, which includes well-organized TJs, expres- sion of nutrient transporters, polarized efflux transporter activity and a high TEER is based on genetically reprogrammed ...this model is ... Voir le document complet

13

Blood-brain-barrier spheroids as an in vitro screening platform for brain-penetrating agents

Blood-brain-barrier spheroids as an in vitro screening platform for brain-penetrating agents

... the brain parenchyma beyond areas of high perfusion (that is, blood vessels), though each CPP demonstrated variation in its level of extravasation across the BBB ...spike in fluorescence ... Voir le document complet

15

From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells

... 016), human nasal olfactory mucosae were obtained by biopsy during routine nasal surgery under general ...placed in growth medium containing DMEM/Ham F12 supplemented with 10% fetal bovine serum (FBS) and ... Voir le document complet

19

Le virus Zika : de sa découverte à nos jours

Le virus Zika : de sa découverte à nos jours

... cérébral in utero sont regroupés sous le terme TORCH (Toxoplasmose, Others [syphilis, VZV, virus B19], Rubéole, Cytomégalovirus et virus de l'Herpès simplex), mais il en existe d’autres, parmi ... Voir le document complet

146

Peripheral Routes to Neurodegeneration: Passing Through the Blood–Brain Barrier

Peripheral Routes to Neurodegeneration: Passing Through the Blood–Brain Barrier

... damage in neurodegenerative disorders has prompted the quest for pharmacological repairing strategies, either directed at the endothelium or by indirect targeting of the cellular players of peripheral and neuro- ... Voir le document complet

15

New insights on ammonia metabolism in endothelial cells of the blood brain barrier

New insights on ammonia metabolism in endothelial cells of the blood brain barrier

... Résumé L'encéphalopathie hépatique (HE) est un syndrome neuropsychiatrique complexe, une complication majeure de la maladie du foie. L'œdème cytotoxique est une complication grave de l'encéphalopathie hépatique, connu ... Voir le document complet

111

The Flavonoid Isoquercitrin Precludes Initiation of Zika Virus Infection in Human Cells

The Flavonoid Isoquercitrin Precludes Initiation of Zika Virus Infection in Human Cells

... illustrated in Figure 4 a, Q3G was added to A549 cells concurrently to virus input, prior to viral infection, or ...with virus input severely reduced the percentage of GFP-positive A549 cells (Figure ... Voir le document complet

14

SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts

SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts

... events in the analysis gate) and for SAMHD1 expression using Western blotting ...bars); virus titers were measured using a plaque assay (gray ...bars); virus titers were measured by plaque assay ... Voir le document complet

19

Blood-brain barrier model on a microfluidic chip for the study of tumor cell extravasation

Blood-brain barrier model on a microfluidic chip for the study of tumor cell extravasation

... To integrate these features into a physiologically relevant blood-brain barrier model, we designed an in vitro microvascular network platform formed via vasculogenesis[r] ... Voir le document complet

58

The pericyte–glia interface at the blood–brain barrier

The pericyte–glia interface at the blood–brain barrier

... HAL Id: hal-02074159 https://hal.archives-ouvertes.fr/hal-02074159 Submitted on 5 Jun 2019 HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether ... Voir le document complet

15

Zika Virus Strains Potentially Display Different Infectious Profiles in Human Neural Cells

Zika Virus Strains Potentially Display Different Infectious Profiles in Human Neural Cells

... involved in anti-viral responses (see Material and Methods and Supplemental Table ...modulated in NSCs upon ZIKV AF infection (Figures 3 A and C). In particular, 19 genes were upregulated, among them ... Voir le document complet

34

Feedback control of microbubble cavitation for ultrasound-mediated blood–brain barrier disruption in non-human primates under magnetic resonance guidance

Feedback control of microbubble cavitation for ultrasound-mediated blood–brain barrier disruption in non-human primates under magnetic resonance guidance

... consists in running a sham experi- ment, that is to say recording broadband backscattered signals as a function of electrical ...setup in feedback control mode except for the micro- bubble ...trapped ... Voir le document complet

15

Magnetic Resonance Methods for Focused Ultrasound-Induced Blood-Brain Barrier Opening

Magnetic Resonance Methods for Focused Ultrasound-Induced Blood-Brain Barrier Opening

... [(A–C) in the ...operate in a clinical ...employed in several clinical trials to permeabilize the BBB in patients with Alzheimer disease [ 32 ] 1,2,3 , Glioblastoma (GBM) [ 17 ] 4,5,6 , ... Voir le document complet

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