[PDF] Top 20 A GFP-based reporter system to monitor nonsense-mediated mRNA decay
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A GFP-based reporter system to monitor nonsense-mediated mRNA decay
... of a premature translation-termination codon (PTC) are recognized and degraded in eukaryotic cells by a process called nonsense-mediated mRNA decay ...of a reporter ... Voir le document complet
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Composition et dynamique des complexes protéiques impliqués dans le "nonsense-mediated mRNA decay" chez la levure Saccharomyces cerevisiae
... defined based on literature data (Fukuhara et al, 2005; Luke et al, ...chosen based on a Mafft alignment of Smg5/6 of several species, while for Nmd4 the entire protein sequence was ...as a ... Voir le document complet
193
A GFP-based assay for rapid screening of compounds affecting ARE-dependent mRNA turnover
... rapid decay giving a low baseline of GFP fluores- ...this system we have identified okadaic acid as a novel stabilizing compound, and investigated the upstream signaling pathways ... Voir le document complet
8
The Complex Relationship between HTLV-1 and Nonsense-Mediated mRNA Decay (NMD)
... seems to be more resistant to h3AG than HIV-1, but interestingly h3AG could generated nonsense mutations in the viral genome that enable HTLV-1-infected cells to escape from the host immune ... Voir le document complet
21
The role of mRNA metaboism in NPM-ALK-mediated oncogenesis
... re-localize to SGs upon ...detected to a lesser extent in PBs (Eulalio et ...re-localization to SGs may reflect the cross-talk between these 2 structures to determine mRNA fate: ... Voir le document complet
244
GC content shapes mRNA decay and storage in human cells
... is a well-characterized direct DDX6 partner known for its involvement in mRNA decay [9,26– ...found a negative correlation between mRNA stabilization after PAT1B and after DDX6 ... Voir le document complet
32
An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins.
... mutated to eliminate a KpnI restriction enzyme site for our subsequent ...cloning. To generate monomeric forms of YPet and CyPet, alanine 206 was substituted with lysine, as reported previously for ... Voir le document complet
11
mRNA fusion constructs serve in a general cell‐based assay to profile oligonucleotide activity
... the reporter gene, all extended in the 3¢-UTR region, but with a constant open reading frame and therefore with expression of a common reporter ...helps to render the assay general with ... Voir le document complet
11
GC content shapes mRNA storage and decay in human cells
... is a well-characterized direct DDX6 partner known for its involvement in mRNA decay ( Vindry et ...found a negative correlation between mRNA stabilization after PAT1B and after DDX6 ... Voir le document complet
33
Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins
... part, to a reduction of pH quenching at low ...cycloheximide to stop the synthesis of GFP, degradation of the lytic vacuolar marker was more ...on GFP-Chi and GFP-KDEL and the ... Voir le document complet
8
The transcription factor ERG recruits CCR4-NOT to control mRNA decay and mitotic progression.
... doxycycline to express ectopic tagged proteins at levels similar to those of endogenous proteins (5, 5 and 500 ng/ml, respectively, for 24 ...Owing to low signals in immunoprecipitates, RNA was ... Voir le document complet
13
Ground-based optical atomic clocks as a tool to monitor vertical surface motion
... sitive to geopotential changes) and gravimeters (sensitive to the downward component of g) each measure a different linear combi- nation of the Love ...desirable to combine gravimeter and ... Voir le document complet
5
Gustave Courbet, reporter ?
... on a repéré ces principes de restriction (au demeurant pas assez étudiés), doit-on « admettre la plénitude virtuelle d’un monde de discours ininterrompus » ...qui a précédé et ce par quoi s’est établie la ... Voir le document complet
23
A New Protein-Protein Interaction Sensor Based on Tripartite Split-GFP Association
... of a blank sample (150 ml of GFP1–9 OPT, ...using a LEICA DM-RB fluorescence microscope, with a 403 and 1003 oil immersion objective to visualize the stained ...with a Photometric ... Voir le document complet
20
Design of sensor system to monitor napkin usage for sustainable user behavior
... The angle at which a user grabs a napkin, the force that a user yanks a napkin, the speed that a user pulls a napkin, and the level of napkins inside of the dispenser can all [r] ... Voir le document complet
39
An improved method to monitor the health of seagrass meadows based on kriging.
... Pergent-Martini, C., Leoni, V., Pasqualini, V., Ardizzone, G.D., Balestri, E., Bedini, R., Belluscio, A., Belsher, T., Borg, J., Boudouresque, C.F., Boumaza, S., Bouquegneau, J.M., Buia, M.C., Calvo, S., Cebrian, ... Voir le document complet
5
Dynamic hybrid reliability studies of a decay heat removal system
... includes a combination of conventional reliability compositional analysis tools and the customized Pythonic objects oriented stochastic hybrid automata ...method. A case study on dynamic reliability studies ... Voir le document complet
23
Statistic-based method to monitor belt transmission looseness through motor phase currents
... due to their high efficiency and their low ...order to identify spectral ...state to detect belt looseness because it exacerbates belt ...proposed based on the application of a ... Voir le document complet
10
Statistic-based method to monitor belt transmission looseness through motor phase currents
... due to their high efficiency and their low ...order to identify spectral ...state to detect belt looseness because it exacerbates belt ...proposed based on the application of a ... Voir le document complet
11
Reprogramming the Dynamin 2 mRNA by Spliceosome-mediated RNA Trans-splicing
... exposed to the following primary antibodies: mouse monoclonal anti-Flag (Sigma F1804), rabbit polyclonal anti-DNM2 (Abcam ab3457, Cam- bridge, UK) in PBS-Tween20 ...submitted to western ... Voir le document complet
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