Haut PDF Expression and regulation of microsomal prostaglandin E synthase-1 in human osteoarthritic cartilage and chondrocytes
Expression and regulation of microsomal prostaglandin E synthase-1 in human osteoarthritic cartilage and chondrocytes
... In addition to inhibition of MMP and NO production, the PPARy activator 1 5d-PGJ2 inhibit IL-1 f3 inducted PGE2 production and COX-2 expression by human chondrocytes (Fahmi et ai, 2002) [r] ...
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Regulation of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes
... detailed in the manufacturer's guidelines. One-fiftieth of the reverse transcriptase reaction product was analyzed by traditional PCR or real-time quantitative ...sense and antisense specific primers ...
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Regulation of lipocalin prostaglandin-D synthase expression by interleukin-1β in human chondrocytes
... studied in different cell types including chondrocytes ...increase in aggrecan messenger ribonucleic acid (mRNA) and a decrease metalloproteinase 3 mRNA by quantitative polymerase chain ...
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Aldose Reductases Influence Prostaglandin F 2α Levels and Adipocyte Differentiation in Male Mouse and Human Species
... transient and maximal mRNA levels coinciding with the onset of dif- ferentiation and decreasing ...completion of the adipogenic program. Although both aldose reductases-b3 and -b7 were ...
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Ndae1 Expression and Regulation in Drosophila Embryos
... nature of these dorsal cells we performed double labeling with anti-Mef2 antibodies, which label all mesodermal cells including those of the heart where expression of Ndae1 has been ...
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Ndae1 Expression and Regulation in Drosophila Embryos
... nature of these dorsal cells we performed double labeling with anti-Mef2 antibodies, which label all mesodermal cells including those of the heart where expression of Ndae1 has been ...
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Mayaro Virus Infects Human Chondrocytes and Induces the Expression of Arthritis-Related Genes Associated with Joint Degradation
... Articular cartilage is a tissue mainly composed of chondrocytes, enclosed in an extracellular matrix (ECM) ...synthesis and maintenance of all ECM molecules [28–30], thereby ...
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Regulation of membrane-type 1 matrix metalloproteinase expression by zonula occludens-2 in human lung cancer cells.
... made in RIPA buffer (50 mmol/L Tris pH7.4, 150 mmol/L NaCl, 1 % (v/v) I- gepal, Triton 1 %, 1 % (w/v) sodium deoxycholate, ...SDS, and 5 mmol/L iodoacetamid) supplemented with complete ...
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Chondrocyte gene expression and intracellular signaling pathways in cartilage mechanotransduction
... The objectives of this thesis were to quantify temporal gene expression changes in chondrocytes within their native extracellular matrix, induced by a set of mecha[r] ...
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Cystathionine-β-synthase: molecular regulation and pharmacological inhibition
... organization and structure of hCBS. (A) Human CBS consists of three architectural ...residues 1–70 contains two distinct ...role in transient Figure 2. Domain organization ...
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Altered pattern of Cul-1 protein expression and neddylation in human lung tumors: relationships with CAND1 and cyclin E protein levels
... role of Cul-1 in human lung carcinogenesis, we studied the relationships between its IHC status and the KI67 proliferative ...index in all tumors tested (p=0.005). These results ...
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Production of interleukin-1 receptor antagonist by human articular chondrocytes.
... production in articular chondrocytes IL-1Ra production was assessed in culture supernatants and lysates of chondrocytes incubated with various ...isolated chondrocytes ...
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Protein-protein interactions and gene expression regulation in HTLV-1 infected cells.
... (19-20), and c-Jun (21) interact with Tax and can redundantly serve to mediate Tax-activation of the ...CREB and Tax associate with the viral 21-base-pair repeat as dimers ...effect of ...
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Altered pattern of Cul-1 protein expression and neddylation in human lung tumors: relationships with CAND1 and cyclin E protein levels The Journal of Pathology
... neddylation and total expression level in NE lung tumors. In contrast , we found that the Cul-1 inhibitor CAND1 is strongly downregulated in HGNE lung carcinoma as compared to ...
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Syndecan-4 is Increased in Osteoarthritic Knee, but not Hip or Shoulder, Articular Hypertrophic Chondrocytes
... KO mice or injected with neutralizing antibody No cartilage degradation in DMM model (same as observed in ADAMTS5 KO mice).. Objectives. 1) Localization by immunohistochemistry in knee[r] ...
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Differential regulation of RANTES and IL-8 expression in lung adenocarcinoma cells.
... indicated in the figure legends and lysed in 10 mM Tris-HCl, pH ...pyrophosphate, 1% Triton X-100 v/v, 1mM EDTA, 1 mM EGTA, 20 mM β- glycerophosphate, ...acid, 1 mM ...
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Syndecan-4 is Increased in Osteoarthritic Knee, but not Hip or Shoulder, Articular Hypertrophic Chondrocytes
... syndecan-4 level was increased by 2.8 fold in knee hypertrophic chondrocytes culture 242. (p=0.0014, Fig 5), compared to non-hypertrophic knee chondrocyte while no differenc[r] ...
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Syndecan-4 is Increased in Osteoarthritic Knee, but not Hip or Shoulder, Articular Hypertrophic Chondrocytes
... palate and brachydactyly of the IV finger of both hands) and parents reported a previous episode of OCD when he was 12: at that time, symptoms resolved with non-weightbearing and ...
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Determinants of human adipose tissue gene expression: impact of diet, sex, metabolic status, and cis genetic regulation.
... parameters of the metabolic syndrome and delay the onset of diabetic complications ...player in the homeostatic control of whole body ...methylome and microRNA arrays, gene ...
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Cartilage mechanobiology and transcriptional effects of combined mechanical compression and IGF-1 stimulation on bovine cartilage explants
... When transcript levels were examined in response to static loading, type II collagen and aggrecan were shown to initially peak anywhere from 1 to 4 hours after compression, fo[r] ...
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