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[PDF] Top 20 DNA sense-and-respond protein modules for mammalian cells

Has 10000 "DNA sense-and-respond protein modules for mammalian cells" found on our website. Below are the top 20 most common "DNA sense-and-respond protein modules for mammalian cells".

DNA sense-and-respond protein modules for mammalian cells

DNA sense-and-respond protein modules for mammalian cells

... synthetic protein components that can detect specific DNA sequences and subsequently trigger a desired intracellular ...finger DNA-­‐recognition to drive the intein-­‐mediated splicing of an ... Voir le document complet

42

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

... plasmid DNA. Typical protocols use about 1–1.25 lg plasmid DNA per ml ...culture. DNA is thus a major cost-factor for large-scale ...coiled DNA and the elimination of ... Voir le document complet

8

Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.

Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.

... in mammalian genomes is to investigate the existence of a N6-DNA methyltransferase in mammalian ...coding for a putative N6- DNA methyltransferase, has been found in murine and ... Voir le document complet

24

Therapeutic glycoprotein production in mammalian cells

Therapeutic glycoprotein production in mammalian cells

... Food and Drug Administration; EMA, European Medicines agency; HEK, human embryonic kidney; shRNA, short hairpin RNA; siRNA, small interference RNA; α-gal, galactose-α1,3-galactose; Neu5Gc, N-glycolylneuraminic ... Voir le document complet

14

How cells respond to environmental cues -insights from bio-functionalized substrates

How cells respond to environmental cues -insights from bio-functionalized substrates

... types, and migration speed and migration persistence of 50 cell types has been probed on one-dimensional (1D) ECM-coated ...2D and 3D in vitro and in vivo environments has led to a ... Voir le document complet

12

CRISPR transcriptional repression devices and layered circuits in mammalian cells

CRISPR transcriptional repression devices and layered circuits in mammalian cells

... framework for a large library of composable devices include the creation of synthetic transcriptional modifiers by fusing effector domains to zinc-finger proteins or transcription activator–like effector (TALE) ... Voir le document complet

12

The cohesin complex is required for the DNA damage-induced G2/M checkpoint in mammalian cells.

The cohesin complex is required for the DNA damage-induced G2/M checkpoint in mammalian cells.

... mediator protein 53BP1 differed significantly between control and Scc1 depleted ...control and 75 % of sororin depleted cells showed 53BP1 staining almost exclusively in IRIFs and ... Voir le document complet

14

The mitochondrial unfolded protein response in mammalian physiology

The mitochondrial unfolded protein response in mammalian physiology

... our cells in ...chondrial DNA, which encodes 13 subunits of the oxidative phosphorylation multiprotein complexes in the electron transport chain (ETC), while the remaining *80 ETC components are encoded in ... Voir le document complet

10

The cumate gene-switch: a system for regulated expression in mammalian cells

The cumate gene-switch: a system for regulated expression in mammalian cells

... expression and therefore CymR-DNA binding, it is the molecule we have used to regulate expression from the mammalian expression system incorporating ...expression, mammalian cell growth is not ... Voir le document complet

18

Antibody-Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections, and Mammalian Embryos

Antibody-Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections, and Mammalian Embryos

... 1.4. DNA Methylation Immunocytochemistry on Cells Antibodies raised against the 5-methylcytosine group and its derivatives have in most cases selective affinity for these epitopes on denatured ... Voir le document complet

23

Advantages and drawbacks of nanospray for studying noncovalent protein-DNA complexes by mass spectrometry

Advantages and drawbacks of nanospray for studying noncovalent protein-DNA complexes by mass spectrometry

... Plasmids and DNA manipulations PET (Novagen) containing the T7 promotor under the control of the lacI repressor, was used as a vector for the overexpression of the BlaIWT, BlaIGM2 and ... Voir le document complet

28

Cooperative nutrient accumulation sustains growth of mammalian cells

Cooperative nutrient accumulation sustains growth of mammalian cells

... uptake and utilization for macromolecular synthesis is central for cell ...metabolic and signaling requirements that impact cell growth on long time scales, whether the same regulation ... Voir le document complet

9

Looping and clustering model for the organization of protein-DNA complexes on the bacterial genome

Looping and clustering model for the organization of protein-DNA complexes on the bacterial genome

... account for the formation of a large coherent protein-DNA complex, given physiological protein inter- action strengths ...along DNA at genomic distances from parS as large as observed ... Voir le document complet

14

Uptake and release of double-walled carbon nanotubes by mammalian cells

Uptake and release of double-walled carbon nanotubes by mammalian cells

... uptake and fate of DWNTs. Figure 1c and 1 d present the Raman spectra of pristine and RNA- wrapped oxidized double-walled carbon nanotubes (DWNT and oxDWNT-RNA, respectively) with two ... Voir le document complet

9

Electro-Mechanical Manipulation of Mammalian Cells in Suspension

Electro-Mechanical Manipulation of Mammalian Cells in Suspension

... U937 and other cell-types CHO cells were found to be stiffer than U937 cells, and less easily deformed by electrical stresses during ...CHO cells compared with U937 cells (table ... Voir le document complet

149

Evaluation of a Microwave Biosensor for On-Chip Electroporation and Efficient Molecular Delivery Into Mammalian Cells

Evaluation of a Microwave Biosensor for On-Chip Electroporation and Efficient Molecular Delivery Into Mammalian Cells

... living cells might have a considerable therapeutic ...treatments and physical approaches, ...amplitude and duration) [2] in order to transiently permeabilize the plasma membrane of ...entry ... Voir le document complet

10

MeCP2-E1 isoform is a dynamically expressed, weakly DNA-bound protein with different protein and DNA interactions compared to MeCP2-E2

MeCP2-E1 isoform is a dynamically expressed, weakly DNA-bound protein with different protein and DNA interactions compared to MeCP2-E2

... MeCP2‑E1 and MeCP2‑E2 isoforms The differences described between E1 and E2 in terms of their affinity for methylated DNA and dynamics might have an influence on and/or reflect a ... Voir le document complet

17

Ultrastructural localization and distribution of Nardilysin in mammalian male germ cells

Ultrastructural localization and distribution of Nardilysin in mammalian male germ cells

... apparatus and ER cisternae. A possible explan- ation for the lack of labelling on ER and Golgi saccules could be that the antibody obtained against the mature protein recognized only this ... Voir le document complet

12

Strategies for Selection from Protein Libraries Composed of de Novo Designed Secondary Structure Modules

Strategies for Selection from Protein Libraries Composed of de Novo Designed Secondary Structure Modules

... display, protein fold ...a protein with a length of 100 amino acids is 20 100 ≈ 10 130 ...more, and thus it is impossible that nature has explored all of the sequence space available to ...to ... Voir le document complet

7

Culturing Aerobic and Anaerobic Bacteria and Mammalian Cells with a Microfluidic Differential Oxygenator

Culturing Aerobic and Anaerobic Bacteria and Mammalian Cells with a Microfluidic Differential Oxygenator

... oxygen and nitrogen input gases, the dissolved oxygen (DO) concentration in proxi- mal microchannels filled with culture media are precisely regulated by molecular ...platform for both aerobic and ... Voir le document complet

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