[PDF] Top 20 Development and Applications of CRISPR-Cas9 for Genome Engineering
Has 10000 "Development and Applications of CRISPR-Cas9 for Genome Engineering" found on our website. Below are the top 20 most common "Development and Applications of CRISPR-Cas9 for Genome Engineering".
Development and Applications of CRISPR-Cas9 for Genome Engineering
... insert, and modify the DNA sequences of cells or organisms enable dissecting the function of specific genes and regulatory ...interrogation of gene or protein networks at a larger ... Voir le document complet
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Viral Vectors for Plant Genome Engineering.
... phenotype of reduced purple pigmentation in anthocyanin-accumulating organs, such as flower ...progeny, and some of these were inherited by at least two further ...requirement for ... Voir le document complet
6
Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes
... Modification of the genome in large animals has always been a difficult task, with few efficient technologies available until recently [ 1 – 4 ...generation of genetically modified large animals has ... Voir le document complet
19
Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system
... PAM for protospacer adjacent ...recruitment of Cas9 to the targeted genomic DNA sequence leading to DNA double strand breaks ...case of DSB repair by non homologous end joining (NHEJ), small ... Voir le document complet
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CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato
... The CRISPR-SaCas9 tools used and developed in this study broaden the scope of genome edit- ing applications for potato, but also for dicot species in ...use of ... Voir le document complet
12
Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9
... principle, engineering complementary CRISPR-Cas sys- tems from distinct bacterial species should be relatively straightfor- ward, as they have been minimized to only two ...reprogrammed for DNA ... Voir le document complet
13
WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat
... Although CRISPR/Cas9 applications promise to accel- erate the pace and course of crop improvement [ 5 , 6 ], a number of hurdles exist that limit full exploitation of this ... Voir le document complet
9
CRISPR-Cas9 pour l'édition de génomes viraux et l'étude des gènes du phage virulent p2
... its CRISPR arrays to defend itself against further phage infections (Barrangou et ...demonstration of the adaptive immunity provided by CRISPR-Cas systems was soon extrapolated to prevent invasion by ... Voir le document complet
194
CRISPRseek: A Bioconductor Package to Identify Target-Specific Guide RNAs for CRISPR-Cas9 Genome-Editing Systems
... Abstract CRISPR-Cas systems are a diverse family of RNA-protein complexes in bacteria that target foreign DNA sequences for ...Derivatives of these complexes have been engineered to cleave ... Voir le document complet
8
CRISPR-Cas9–based treatment of myocilin-associated glaucoma
... elevation and also reduces IOP in Tg-MYOC Y437H ...model and has Food and Drug Administration approval as an oral agent for urea cycle disorders, the continuous production of mu- tant ... Voir le document complet
7
Applications of the CRISPR–Cas9 system in cancer biology
... Permanent Cas9-mediated modification of single or multiple endogenous loci can be achieved via transient or stable delivery of the CRISPR ...editing of endogenous genes in cells in ... Voir le document complet
27
Gene Editing in Trypanosomatids: Tips and Tricks in the CRISPR-Cas9 Era
... limitations of the chosen method and design appropriate controls to enable its ...used for gene tagging in trypanosomatids in the pre- CRISPR-Cas9 ...Leishmania and T. cruzi, ... Voir le document complet
16
CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
... while Cas9/sg17 was efficient in suppressing infection and in directly cleaving nuclear cccDNA, Cas9/sg21 efficiently cleaved only integrated but not episomal DNA, which resulted in a lack of ... Voir le document complet
10
CRISPR/Cas9-Induced (CTG⋅CAG)n Repeat Instability in the Myotonic Dystrophy Type 1 Locus: Implications for Therapeutic Genome Editing
... complexity and our still unripe knowledge about the significance of these pathobiological mechanisms, it is not surprising that the development of therapy that could stop the cellular problems ... Voir le document complet
34
en fr Axonal target specificity in the CRISPR/Cas9 era : a new role for Reelin in vertebrate visual sytem development Spécificité du ciblage axonale dans l'ère du CRISPR/Cas9 : un rôle nouveau pour la Reelin pendant le développement du système visuel chez les vertébrés
... composed of eight repeats, each formed by two sub-repeats (A and B) separated by a central epidermal growth factor (EGF) ...required for secretion, a following region with similarity to the ... Voir le document complet
170
Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor
... Abstract: Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop ...precise and predictable targeted point mutations valuable for crop ... Voir le document complet
11
Targeted mutagenesis in wheat microspores using CRISPR/Cas9
... using CRISPR/Cas9 Pankaj Bhowmik 1 , Evan Ellison 2 , Brittany Polley 1 , Venkatesh Bollina 1 , Manoj Kulkarni 1 , Kaveh Ghanbarnia 1 , Halim Song 1 , Caixia Gao 3 , Daniel ...1 CRISPR/Cas9 ... Voir le document complet
11
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
... Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA 4 Department of Genetics, Harvard Medical School, Boston, MA 02115, USA Abstract Targeted genome ... Voir le document complet
20
CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
... to CRISPR-Cas9-mediated gene therapy. Using CEP disease as a proof of concept, we demonstrate that the single nickase method is the best for homozygous mutation ...elements of the ... Voir le document complet
15
Improved CRISPR/Cas9 Tools for the Rapid Metabolic Engineering of Clostridium acetobutylicum
... 2 of 11 expression of cas9 in several different clostridia; however, this system was not tested in ...]. Cas9 nickase, in which Cas9 has been mutated so that it can only cut one strand ... Voir le document complet
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