[PDF] Top 20 The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation

... of cell-secreted signals are soluble signals that travel through the culture media to signal to other cells in the environment, but cells can also signal through direct contact with other ... Voir le document complet

... Biology and b Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139 Edited by Janet Rossant, Hospital for Sick Children, University of Toronto, Toronto, ON, ... Voir le document complet

... Matsumoto and Maller, 2004 ); and pSport1- tetCMVhumCycE WT pA-INS-Hygro (expressing a human WT cyclin E ...under the control of the doxycyclin (Dox) inducible promoter ...antibiotics, ... Voir le document complet

... cells, the core pluripotency genes were characterized by specific knockdown strategies [ 40 , 41 ...muscle and microspikes RAS (Mras), Esrrb and T cell lymphoma breakpoint 1 (Tcl1)] whose ... Voir le document complet

... LIF and formation of embryoid bodies for 1 to 6 ...pim-2, and pim-3. Expression of Oct-4 was used as control of differentiation and ß-actin as control of the RT-PCR ...pim-1 and ... Voir le document complet

... Cancer stem cells refer to a subset of tumor cells that has the ability to self-renew and generate tumor heterogeneity [3; 4; ...for the self-renewal and ... Voir le document complet

... potential and its variation with the number of cells in a cluster We next decided to investigate a more comprehensive measure of ...of self‐renewal. Since the process of ... Voir le document complet

... during the lows – my fellow Singaporeans, who I have subjected to many culinary experiments and hijacked conversations steered towards research; and the graduate community of Sidney-Pacific, ... Voir le document complet

... in the chip with a syringe pump (Pump 11 Elite from Har- vard Apparatus, Holliston, Unisted States) in a withdrawal mode with a flowrate of 20 µL/h small enough to avoid any perturbations inside the ... Voir le document complet

... The PD-1/PD-L1 axis in RCC PD-1 PD-1 is an inhibitory receptor of the CD28/B7 superfamily, upregulated on lymphocytes upon TCR or BCR engagement or γ-chain cytokine-mediated activation (353) (354) ... Voir le document complet

... of the basal line- age, precludes the use of transcriptomic or other profiling strategies to identify candidate regulators that commit bipotent cells to the basal ...transplantation and ... Voir le document complet

... ES cell lines and ChIP was performed sequentially using anti H3K4me3 and then anti-H3K27me3 ...Specific Embryonic Cardiac ...lysed and sonicated. The anti-H3K27ac antibody was ... Voir le document complet

... for the following strategy: 612 E4 embryos were microdissected to isolate the ...murine embryonic fibroblasts ...[S]); and (3) growth fac- tors (FGF2 [F], LIF [L], or growth factor-free [gff]) ... Voir le document complet

... show the function of Shavenbaby in enterocytes, but not in enteroendocrine cells, consistent with an early separation between EC and EE lineages (Biteau & Jasper, 2014, Guo & Ohlstein, 2015, Zeng ... Voir le document complet

... testicular and esophageal tumors, in which they are abundant, miR-372 and miR-373 have been reported to act as oncogenes repressing LATS2, a serine-threonine kinase involved in cell cycle regulation ... Voir le document complet

... fluency. The cells were cross-linked by adding fresh 11% for- maldehyde solution to the ES media for 10 minutes at room ...temperature. The cells were washed twice with 1× phosphate- buffered saline ... Voir le document complet

... siRNAs and miRNAs bind to Argonaute/Piwi family proteins of the Ago sub-type which are widely expressed in different tissue types (Wutz ...domains: the PAZ, MID and PIWI domains. The ... Voir le document complet

... for the nucleation of the autophagosome (Kyei et ...is the central degradation process in cells required for the recycling of damaged organelles and macromolecules (Rubinsztein et ... Voir le document complet

... Sampling and Analysis Proteins were extracted by tissue disruption in ice-cold PBS containing protease ...inhibitors. The protein concentration was assessed using the Pierce BCA Protein Assay Kit ... Voir le document complet

... explain the lack of NK cells in the tumor. Indeed, we could find that the production of various chemokines was modified in the tumor compared to the adjacent healthy ...cells, ... Voir le document complet