[PDF] Top 20 Cell-free protein synthesis of perdeuterated proteins for NMR studies
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Cell-free protein synthesis of perdeuterated proteins for NMR studies
... Abstract Cell-free protein synthesis protocols for uniformly deuterated proteins typically yield low, non- uniform deuteration ...coli cell-extract, D-S30, which enables ... Voir le document complet
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Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
... concentrations of salt ( Schlicksup et ...thus of high interest. This can principally be achieved using cell-free protein synthesis ...CFPS of the HBV capsid has been ... Voir le document complet
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Phosphorylation and Alternative Translation on Wheat Germ Cell-Free Protein Synthesis of the DHBV Large Envelope Protein
... purification of DHBs L using the StrepTag on the C-terminal or N-terminal end are shown as well in Figure ...that for DHBs L with the StrepTag at the C-terminal end, two separate bands are observed ... Voir le document complet
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Automated Protein NMR Structure Determination in Crude Cell-Extract
... overexpression of the target pro- tein is needed to perform an automated structure determination with the protocol used here for ...use for less favorable proteins may be achieved with refined ... Voir le document complet
9
NMR methods for intrinsically disordered proteins : application to studies of NS5A protein of hepatitis C virus
... 4.3. NMR OBSERVABLES FOR IDPS 33 properties of denatured states and short peptides ...[59]. Protein regions that have no secondary or tertiary structure, as common in intrinsically disordered ... Voir le document complet
259
Real-Time NMR Studies of Electrochemical Double-Layer Capacitors
... the NMR coil. The 11 B in situ NMR spectra of the supercapacitor charged from 0 to 2 V and charged from 0 to -2 V (Figure 4a - c) now show the individual spectra of the posi- tive and negative ... Voir le document complet
6
Diffusive MASS NMR Studies of Transport in Porous Materials
... dynamics of porous materials including such diverse materials as cheese and chocolate, cosmetics and pharmaceuticals, solvents in resins and soft matter, biological tissue, and for oil ...exploration. ... Voir le document complet
6
Real-Time NMR Studies of Electrochemical Double-Layer Capacitors
... demand for energy has in- creased dramatically. With the increased use of renewable energy sources comes the challenge as to how energy can be stored and ...rates of charge and discharge, and their ... Voir le document complet
7
Hybrid Image-based Rendering for Free-view Synthesis
... baseline for color correction, since it provides a stable common reference point for appearance across input views without view-dependent ...color for each vertex and is typically free from ... Voir le document complet
21
Fast and ergonomic extraction of adherent mammalian cells for NMR-based metabolomics studies
... recovery of the cell debris unessential. Three groups of samples were assessed and the corresponding results are presented in Figure ...4. For the first group, 3 ml methanol was spread across ... Voir le document complet
24
Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins
... ated proteins for NMR studies and structure ...Expressed Protein Liga- tion and Protein ...limit of NMR spectroscopy further making larger proteins ... Voir le document complet
15
Partition of tRNAGly isoacceptors between protein and cell-wall peptidoglycan synthesis in Staphylococcus aureus
... found for homologous tRNAs from ...absence of pseudouridine, which is ubiquitous in the T loop, was ...sequence of the three non-proteogenic tRNA Gly genes contains the TGC or TGT triplet in the T ... Voir le document complet
17
Reconciling NMR Structures of the HIV-1 Nucleocapsid Protein NCp7 Using Extensive Polarizable Force Field Free-Energy Simulations
... need for additional external pulling ...preference for the unstacked form ...ns of simulation and not recovered after 100 ...lization of B due to dispersion and long-range polarization effects ... Voir le document complet
25
Solvent-free mechanochemical route for green synthesis of pharmaceutically attractive phenolhydrazones
... ratios of 85–99% were obtained depending on the hydrazine/aldehyde used, but it was demonstrated that the reactions could reach trans- formations $99% by changing the time of grinding, as illus- trated in ... Voir le document complet
8
Deadlock-free Discrete Controller Synthesis for Infinite State Systems
... Control of Infinite Systems The control of infinite systems has been subject to several ...control of Timed Automata [4], or Vector of Discrete Events Systems ...case of Petri Nets, ... Voir le document complet
9
Characterization of structure and dynamics of membrane proteins from solid-state NMR
... Solid-state nuclear magnetic resonance (ssNMR) spectroscopy is an essential tool for elucidating the structure, dynamics, and function of biomolecules. ssNMR is capable [r] ... Voir le document complet
144
Modeling of a Cell-Free Synthetic System for Biohydrogen Production
... properties of the cell-free system of Ye et ...effectiveness of the method needs further validation using real experimental ...Other cell-free systems for hydrogen ... Voir le document complet
9
GeNMR: a web server for rapid NMR-based protein structure determination
... (GEnerate NMR structures) is a web server for rapidly generating accurate 3D protein struc- tures using sequence data, NOE-based dis- tance restraints and/or NMR chemical shifts as ...ensemble ... Voir le document complet
9
Synthesis and structure of free-standing germanium quantum dots and their application in live cell imaging
... modelling of small Ge qdots reported ...magnitude of the Fourier transform (MFT) of the k 2 -weighted EXAFS signal, which provides a convenient way to visualize the average local environment around ... Voir le document complet
9
Bimodal detection of proteins by $^{129}$Xe NMR and fluorescence spectroscopy
... study of intracellular biological mechanisms requires sensitive and non-invasive ...use of several analytical methods makes it possible to overcome the limitation of each of ...viewpoint ... Voir le document complet
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