[PDF] Top 20 Analysis of HIV-1 cell-to-cell transfer to macrophages
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Analysis of HIV-1 cell-to-cell transfer to macrophages
... Mosaic of 4X4 confocal fields of original magnification ...The cell fusion index (nuclei number in multinucleated cells/total nuclei number) and the percentage of area covered by ... Voir le document complet
231
Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction
... used to analyze virus dissemination by flow cytometry, since the Gag ⫹ iDC-derived MGCs formed through cell fusion during coculture with infected T cells were certainly excluded from the analysis ... Voir le document complet
19
HIV-1 assembly in macrophages.
... connected to the plasma membrane Despite the numerous evidence showing that HIV-1 assembly occurs in macrophages in MVB-related com- partments, recent studies have challenged this ...usage ... Voir le document complet
11
HIV-1-Infected Human Macrophages, by Secreting RANK-L, Contribute to Enhanced Osteoclast Recruitment
... compared to non-infected ...number of nuclei per MGC was higher in HIV-1-infected MF (Nb of nuclei/cell; 2–4 nuclei: 65 ± 2%, 5–9 nuclei: 26 ± 1%, >10 nuclei: 9 ± ... Voir le document complet
17
Public T cell receptors confer high-avidity CD4 responses to HIV controllers.
... TCR transfer confers Gag293 recognition in the context of multi- ple HLA-DR ...out to functionally characterize the most prevalent TRAV24 public clonotype, AFKAAGNKLT (clonotype TRAV24-F), by pairing ... Voir le document complet
17
Natural sequence variants of yeast environmental sensors confer cell-to-cell expression variability.
... cloning of genomic fragments into such plasmids, which is sometimes not convenient when performed in ...efficiency of integration in the genome. To bypass the limiting in vitro cloning steps ... Voir le document complet
18
Use of silicone membranes to enhance gas transfer during microbial fuel cell operation on carbon monoxide
... absence of power generation from the nutrients present in the influent stream ...output of 3–4 mV (Rext = 500 X , Pout < ...solution of nutrients and microele- ments in the absence of ... Voir le document complet
10
Early CD4+ T Cell Responses Are Associated with Subsequent CD8+ T Cell Responses to an rAd5-Based Prophylactic Prime-Boost HIV Vaccine Strategy
... responses to the vaccine strategies were different between the two arms of HVTN 068 trial, and no correlations between the CD4+ IL-2+ and CD8+ IFN- γ+ responses were observed in the group with the DNA ... Voir le document complet
11
Gene network analysis leads to functional validation of pathways linked to cancer cell growth and survival.
... and to inflammatory response (NFKB and c-Rel). In order to determine whether the 28 transcription factors with significant over-represented binding sites in the promoter region of gene sets 1 ... Voir le document complet
36
Transcriptional regulation of effector CD8+ T cell differentiation and molecular dysfunction during HIV-1 infection
... acquisition of infection that was seen was attributed to non-neutralizing V1/V2 antibodies and not mediated by CD8 cytotoxic T ...goal of current vaccines against HIV is to induce high ... Voir le document complet
192
FOXO1 transcription factor plays a key role in T cell—HIV-1 interaction
... activity of T cells, affecting all T cell subsets, including naive T ...expression of CD71 and CD98 metabolic markers, increased glucose uptake and greater mitochondrial res- piration after AS1842856 ... Voir le document complet
24
Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply
... Vpx to enhance the dNTP pool to the same extent with or without IFN-α since SAMHD1 was degraded in both ...dose of IFN-α was deliberately used to severely inhibit HIV-1 transduc- ... Voir le document complet
12
Cell invasion in the spheroid sprouting assay: a spatial organisation analysis adaptable to cell behaviour.
... Figure 1. Description of the spheroid assay and the method of ...representation of spheroid evolution during cell ...modes of cell sprouting and invasion are depicted on ... Voir le document complet
10
Cell-Intrinsic and cell-extrinsic resistance to classical chemotherapies
... presence of varying dilutions of this CM ...dose of gemcitabine required to achieve 50% reduction in the growth rate of PDAC cells (GR50, a metric which takes into account cytostatic ... Voir le document complet
121
The expression of EMX2 lead to cell cycle arrest in glioblastoma cell line
... blot analysis Total protein was extracted from cells using extraction solution (50 mM Tris pH ...NaCl, 1 mM EDTA, 1% NP40 and 1 mM DTT) supplemented with protease inhibitors (Thermo Fisher ... Voir le document complet
12
A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome
... proteins of interest include Tbc1d10, an activating protein of the GTPase ...formation of the immunological synapse, by regulating TCR ...knockdown of the former inhibits immunological synapse ... Voir le document complet
45
Biophysical forces rewire cell metabolism to guide microtubule-dependent cell mechanics
... at 1/500 for confocal imaging, while Alexa 594 and Star Red (Abberior) were used at 1/100 for STED ...laser to excite the Alexa594 at 561nm and the StarRed at ... Voir le document complet
44
Nonlinear ultrasound parameter to monitor cell death in cancer cell samples
... detection of tumor response to anti-cancer therapy is a major challenge in the fight against ...methods of assessing therapy effects during patient treat- ment are often invasive, requiring biopsied ... Voir le document complet
7
CD8⁺ T Cell Response to Influenza Virus
... Antigen specific CD8+ T lymphocytes are selected and activated in the mediastinal lymph node, proliferate and gain effector function, then migrate to the lungs, whe[r] ... Voir le document complet
12
Retinal cell type expression specificity of HIV-1-derived gene transfer vectors upon subretinal injection in the adult rat: influence of pseudotyping and promoter.
... Use of Animals in Ophthalmic and Vision ...(2 to 3 months old) rdy rats (kindly provided by Dr ...University of California, San Francisco, ...injection of ketamine/xylazine. Eyes were treated ... Voir le document complet
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