virus RNA
Recognition of hepatitis C virus RNA by toll like receptors 7 and 8 : implications for the initiation of innate immune response
... 28 have been made to prove the immunogenicity of HCV particles, but the only common element in these studies is the unresponsiveness of DCs to HCV stimulation. Neither DC maturation nor induction of cytokines was ...
195
Rapid Diagnostic Tests as a Source of Dengue Virus RNA for Envelope Gene Amplification: A Proof of Concept
... for RNA purification using QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to previously published ...Viral RNA Mini Kit following the manufacturer ’s ...
6
Structure, dynamics and phase separation of measles virus RNA replication machinery
... The polymerase cofactor P is tetrameric, consisting of a central coiled-coil domain [3], whose structural properties have recently been linked to MeV gene expression [11], flanked by a long (304 aa) intrinsically ...
25
Detection of Japanese Encephalitis Virus RNA in Human Throat Samples in Laos – A Pilot study
... improve diagnosis, especially in patients in whom a lumbar puncture (LP) is not possible or where LP facilitates are not available. Throat swab collection does not require specific facilities, and therefore could ...
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Performance of the Abbott real-time PCR assay using m2000sp and m2000rt for hepatitis C virus RNA quantification.: m2000SP/m2000RT FOR HCV RNA QUANTIFICATION
... HCV RNA independently of the HCV genotype and has been well calibrated to the World Health Organization (WHO), as confirmed in this study (1, 8, 9, 12, 13, 24, ...HCV RNA levels measured in the two assays ...
28
Viral RNA Degradation Makes Urine a Challenging Specimen for Detection of Japanese Encephalitis Virus in Patients With Suspected CNS Infection
... carrier RNA to fresh urine permitted stabilization of viral RNA for subsequent detec- tion by RT-qPCR even after a freezing ...carrier RNA to fresh samples, permitting the deferment of molecular ...
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Molecular dynamics studies of the nucleoprotein of influenza A virus: role of the protein flexibility in RNA binding
... the RNA binding ...the RNA binding groove and mediates RNA binding and protein self-association, differ- ences should be observed between NP and ...facilitated RNA binding to wt NP, presumably ...
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RNA interference identifies domesticated viral genes involved in assembly and trafficking of virus-derived particles in ichneumonid wasps
... unique virus-derived particles produced by parasitic wasps that rely on them to successfully develop in their insect hosts; however, a viral origin for these particles has only recently become ...used RNA ...
22
In situ Structure of Rotavirus VP1 RNA-Dependent RNA Polymerase
... double-strand RNA viruses, package an RNA-dependent RNA polymerase (RdRp) with each duplex of their segmented ...synthesize RNA, in vitro as well as in vivo, but appears not to induce any ...
16
L’histoire du vivant: Des viroïdes et virus à l’homme, dans les traces du « RNA World »
... « RNA World » ( Flores et ...Certains virus comme celui de l’hépatite D (HDV) existent également sous forme circulaire; HDV se réplique de la même façon que les viroïdes ( Flores et ...
4
Reverse Genetics of RNA Viruses: ISA-Based Approach to Control Viral Population Diversity without Modifying Virus Phenotype
... encephalitis virus as model to demonstrate that utilization of a very high-fidelity, DNA-dependent DNA polymerase during the PCR step of the ISA procedure gives the possibility to reduce the genetic diversity of ...
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Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication
... hexameric RNA binding to a single ...the RNA binding cleft of the two assembled ...the RNA pocket, with their bases accessible to the solvent, followed by a flip in the RNA backbone conforma- ...
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High resolution cryo-EM structure of the helical RNA-bound Hantaan virus nucleocapsid reveals its assembly mechanisms
... viral RNA, but after purification the opti- cal density (OD) at 260–280 nm was measured to be around ...with RNA phosphates, in line with the versatility of RNA sequence to be incorpo- rated by ...
15
Genome-Wide Small Interfering RNA Screens Reveal VAMP3 as a Novel Host Factor Required for Uukuniemi Virus Late Penetration
... interfering RNA (siRNA) libraries to investigate host proteins required during infection of human cells by the bunyavirus Uukuniemi virus (UUKV), a late-penetrating ...ⴙ virus-containing ...
14
RNA Base Pairing Determines the Conformations of RNA Inside Spherical Viruses
... on RNA profiles for κ = 1, κ = ...of RNA inside a viral shell as a function of its length for a fixed capsid-genome interaction (κ) and base-pairing strength ...of RNA on its density profile inside ...
9
In-depth characterization of the NS3:NS5 interaction within the West Nile virus replicase complex during positive strand RNA synthesis
... mutant virus could be a vaccine ...infectious virus instead of the replicon, and since WNV is a biosafety level 3 pathogen, these experiments might be a bit more difficult to ...
97
High virulence does not necessarily impede viral adaptation to a new host: a case study using a plant RNA virus
... the virus population as a real-time indicator of ...cestral virus and evolved lineages were sequenced by Illumina ...for RNA virus evolutionary ...
14
RNA imaging in bacteria
... of RNA requires the labeling of transcripts in vivo ...phage RNA (Bertrand et ...other RNA species. Bertrand and coworkers fused multiple repeats of the MS2 RNA hairpin to the 3’ UTR of a ...
31
Selective targeting and inhibition of yeast RNA polymerase II by RNA aptamers
... yeast RNA polymerase are highly homologous, in particular the two largest subunits, which rep- resent two thirds of the mass of the enzymes, we analyzed the effect of the FC RNA on the in vitro ...
9
RNA-directed DNA methylation
... specialized RNA-based mechanism guides heterochromatin formation and silencing to one of the two X chromosomes in female mouse-embryonic-cells to adjust the dosage of X-encoded gene expression to be equivalent to ...
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