Site directed mutagenesis
Site-Directed Mutagenesis of the Actinomadura R39 DD-Peptidase
... by site-directed mutagenesis of residues chosen on the basis of the three- dimensional structure of the former [9] and of the presence, in all DD-transpeptidases and PBPs of conserved structural ...
7
Molecular modelling and site directed mutagenesis of plant chloroplast MGDG synthase reveal critical residues for activity
... by site-directed mutagenesis, we could obtain the overall fold of an Angiosperm MGDG ...binding site for the UDP- Gal sugar donor was best ...any site dedicated to metal ...
12
Dramatic broadening of the substrate profile of the Aeromonas hydrophila CphA metallo-beta-lactamase by site-directed mutagenesis
... binding site non-competitively inhibits the enzyme with a K i value of 46 µM at pH ...active site is located at the bottom of the β-sheet ...and site-directed mutagenesis studies (16), ...
13
Site-directed mutagenesis of conserved inverted repeat sequences in the xylanase C promoter region from Streptomyces sp EC3
... by site-directed mutagenesis, no protein binding was observed (Giann- otta et ...binding site for a transcriptional ...equivalent. Mutagenesis of box 3 has a strong influence on gene ...
10
Catalytic mechanism of Heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate
... The GlcNAc in ⫹2 subsite adopts a similar conformation and keeps the same interactions with Asn 405 , Gly 470 , Asn 437 , and His 202 as GlcNS6S (Fig. 4d). Moreover, Tyr 436 side chain stacks parallel against the GlcNAc ...
12
Role of the Conserved Amino Acids of the 'Sdn' Loop (Ser130, Asp131 and Asn132) in a Class a Beta-Lactamase Studied by Site-Directed Mutagenesis
... For the active mutant proteins, the enzyme-containing fractions were detected using the nitrocefin assay, whereas fractions containing the nearly inactive S130N and N132A enzymes were de[r] ...
8
Site-Directed Mutagenesis of the Streptomyces R61 Dd-Peptidase. Catalytic Function of the Conserved Residues around the Active Site and a Comparison with Class-a and Class-C Beta-Lactamases
... This result is in contrast with those obtained with class-A p- lactamases, where the replacement of the active Ser residue by a Cys yielded enzymes exhibiting a significant residua[r] ...
6
Structural and catalytic role of arginine 88 in Escherichia coli adenylate kinase as evidenced by chemical modification and site-directed mutagenesis
... ce site Web et l’utilisation de son contenu sont assujettis aux conditions présentées dans le site LISEZ CES CONDITIONS ATTENTIVEMENT AVANT D’UTILISER CE SITE ...
7
Site-directed mutagenesis of glutamate 166 in two beta-lactamases. Kinetic and molecular modeling studies.
... licheniformis E166A mutant but quite different with the TEM-1 E166Y (39) and S. aureus D179N (40) proteins where the loop was disordered. Here the most striking structural modifications were the displacement of the water ...
7
Increasing the synthesis/hydrolysis ratio of aminoacylase 1 by site-directed mutagenesis
... Detailed procedures for the building of structural models for wild-type and mutant pAcy1 are given in Supplementary material . The procedure was exhaustively validated and homology models of wild-type pAcy1 were verified ...
9
Cold adaptation of a psychrophilic chitinase: a mutagenesis study
... Site-directed mutagenesis Mutants were constructed by a two step±four primer overlap/ extension PCR method (Horton et al., 1990). The ampli®ed product was subcloned in pET26b vector downstream of the ...
7
Deconstruction of the Ras switching cycle through saturation mutagenesis
... saturation mutagenesis experiment in the background of the oncogenic G12V mutation, and found that the presence of this mutation reduces the effect of mutations that activate wild-type H-Ras, indicative of ...
32
P-Laplacians on Directed Graphs
... is endowed with the usual inner product hf, hi H( V) = P v i ∈ V f (v i )h(v i ), where f, h : V → R. Similarly, let H(E) be the space of real-valued functions defined on the edges of G. It is endowed with the inner ...
3
The PIG- A gene, a new marker for mutagenesis
... of mutagenesis: proof of concept and technical specifications Gene mutations are not directly detected by current genotoxicity assays and most of them need a cell culture ...of mutagenesis, performed in ...
9
RNA-directed DNA methylation
... Although it remains unclear whether RNA-directed coding- sequence-methylation has functional consequences, it is evident from both transgene and RNA-virus-experiments that dsRNA can also trigger promoter ...
8
Targeted mutagenesis in wheat microspores using CRISPR/Cas9
... target site was PCR-ampliied, cloned into an Invitrogen TOPO-Blunt plasmid and 96 clones were sequenced by Sanger ...cleavage site in 2 out of the 96 sequenced products ...targeted mutagenesis in ...
11
Action Observation Network activity related to object-directed and socially-directed actions in Adolescents
... versus Non-Social actions and in right pSTS for Transitive vs Intransitive actions (see Figure 460. 5B and Extended Data Table 5-1), thus confirming the results obtained in the ROI anal[r] ...
45
WDM and Directed Star Arboricity
... (A(G)4A(P )) ∪ {c 1 a 1 } which spans V (D 0 ) ∪ x. 5 Complexity The digraphs with directed star arboricity 1 are the galaxies. So one can polynomially decide if dst(D) = 1. Deciding whether dst(D) = 2 or not is ...
18
Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe
... active site-directed covalent probes that consist of small molecule inhibitors linked to reporter tags [12] that exploit conserved mechanistic features of their target enzyme ...these directed probes ...
16
On Bubble Generators in Directed Graphs
... the minimum cardinality. 314 Fig. 4: An example showing that conditions (1) and (2) of Theorem 1 are not sufficient to guarantee that a bubble is simple. (a) A directed graph G. (b) The three bubbles B 1 , B 2 and ...
19