SINGLE-STRANDED-DNA
Bound or free: interaction of the C-terminal domain of Escherichia coli single-stranded DNA-binding protein (SSB) with the tetrameric core of SSB.
... ABSTRACT: Single-stranded DNA (ssDNA)-binding protein (SSB) protects ssDNA from degradation and recruits other proteins for DNA replication and ...
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High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes
... INTRODUCTION Single-stranded binding (SSB) proteins bind with a high affinity to single-stranded DNA (ssDNA), which is a transient state in many DNA metabolic processes such as ...
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Two distinct conformational states define the interaction of human RAD51‐ATP with single‐stranded DNA
... of DNA damage is crucial for chromosome integ- rity, since it prevents mutations, chromosomal aberrations and errors in essential processes such as transcription, replication and chromosome segregation ...
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Recombination in eukaryotic single stranded DNA viruses
... cheap DNA synthesis means that the opportunity now exists for us to literally recreate infectious ancestral recombinant virus genomes [257] and use these to directly determine things like their neutralization ...
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Chimeric viruses blur the borders between the major groups of eukaryotic single-stranded DNA viruses.
... Simon Roux 1,2 , Franc¸ois Enault 1,2 , Gise `le Bronner 1,2 , Daniel Vaulot 3 , Patrick Forterre 4,5 & Mart Krupovic 4 Metagenomic studies have uncovered an astonishing diversity of ssDNA viruses encoding ...
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A conserved MCM single-stranded DNA binding element is essential for replication initiation
... solution (10 mg/ml) and 200 nl of well solution (0.2 M sodium malonate, pH 7.0, 20% PEG 3350). A plate crystal was cryoprotected by quickly passing it through well solution containing 15% ethylene glycol and flash frozen ...
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Initiation of DNA double strand break repair: signaling and single-stranded resection dictate the choice between homologous recombination, non-homologous end-joining and alternative end-joining
... A DNA double strand break (DSB) is a highly toxic lesion, which can generate genetic instability and pro- found genome ...by single-stranded DNA (ssDNA) resection and requires sequence ...
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Acheta domesticus Volvovirus, a Novel Single-Stranded Circular DNA Virus of the House Cricket
... The genome of a novel virus of the house cricket consists of a 2,517-nucleotide (nt) circular single-stranded DNA (ssDNA) mole- cule with 4 open reading frames (ORFs). One ORF had a low identity to ...
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Single-strand DNA translation initiation step analyzed by Isothermal Titration Calorimetry.
... 1964, DNA was used in vitro to study translation and the question still remains whether or not DNA could be directly translated into ...whether single-stranded DNA (ssDNA) might be able ...
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Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing
... undesirable DNA contaminants in rAAV preparations remain a major safety ...of DNA fragments containing antibiotic resistance genes, wild-type AAV, and packaging cell genomes has been found in previous ...
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Human RAD52 Captures and Holds DNA Strands, Increases DNA Flexibility, and Prevents Melting of Duplex DNA: Implications for DNA Recombination
... tary single-stranded DNA ...in DNA repair ...with single DNA mole- ...double-stranded DNA (dsDNA), interaction is slower, weaker, and often ...enhancing DNA ...
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Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB)
... of DNA damage, in particular oxidation, hydrolytic deamination, and alkylation ...nuclear DNA has been explored in detail, much less is known about the repair of DNA alkylation damage in ...
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Isolation of TGF-β-neutralizing single-domain antibodies of predetermined epitope specificity using next-generation DNA sequencing
... a single antigen and epi- tope of interest, and the results may vary substantially depending on properties of the antigen, the Abs present in the library and the com- petitor ...
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Stranded assets risk derails Vietnam's plan for new coal power plants
... ii) The natural conditions for renewable energy sources are favorable in Vietnam, there is lots of solar irradiation and lots of wind offshore. Their cost is declining with innovation and economies of scale. After ...
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Isolation of TGF-β-neutralizing single-domain antibodies of predetermined epitope specificity using next-generation DNA sequencing
... Wells were washed 5× with PBS containing 0.1% Tween-20 (PBS-T), incubated with 35 μL horseradish peroxidase (HRP)-conjugated anti-myc antibody (Santa Cruz Biotechnology, Dallas, TX; di[r] ...
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Stranded in Mauritania : sub-saharian migrants in a post transit context
... between august 2005 and May 2006, hundreds of black african migrants arrived in nouadhibou and tried, on board fishermen’s dinghies, to go up the coastline of Western Sahara and land o[r] ...
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Single-fiber studies for assigning pathogenicity of eight unclassified mitochondrial DNA variants associated with mitochondrial cytopathies phenotypes
... 4.1.5.5 Les pseudogènes mitochondriaux ou Nuclear mitochondrial DNA sequences (NUMTs) Ce sont des pseudogènes dans l'ADN nucléaire qui dérivent de l'ADN mitochondrial. Leur taille varie de 39 pb à la ...
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DNA Fluorescence
... of DNA helices is an intricate ...given DNA helix, governed by electronic coupling and associated with the various conformations: * states localized on a single base, Frenkel excitons delocalized ...
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Functions of double-stranded RNA-binding domains in nucleocytoplasmic transport
... Legend: dsRBD other type of domain anchoring factor Figure 2. Functions of double-stranded RNA-binding domains in nucleocytoplasmic transport. dsRBDs (red spheres) are involved in many aspects governing the ...
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Comparative Dynamics and Sequence Dependence of DNA and RNA Binding to Single Walled Carbon Nanotubes
... stable DNA-SWCNT complex, C 30 DNA-SWCNT, compared to the least fluorescently stable complex, (AT) 7 A-(GU) 7 G DNA-RNA ...15 DNA- and (GU) 15 RNA-SWCNT ...to single walled carbon ...
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