single-strand binding protein
Crystal structure of penicillin-binding protein 3 (PBP3) from Escherichia coli
... penicillin-binding protein; peptidoglycan; PBP3; ftsI Abstract: In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing ...
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Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB)
... during strand displacement mtDNA replication [50, ...such strand-asymmetric DNA synthesis is that one of the newly synthetized mitochondrial genomes contains large ssDNA regions ...[52-55]. Binding ...
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Single-strand DNA translation initiation step analyzed by Isothermal Titration Calorimetry.
... the binding constants between S30 extract and specific signal ...Since protein/nucleic acid interactions were mostly observed with purine [23], we chose a polyT as framework for all the oligonucleotides to ...
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Sak4 of Phage HK620 Is a RecA Remote Homolog With Single-Strand Annealing Activity Stimulated by Its Cognate SSB Protein
... RadB protein, a Rad51 ...allowed single-strand annealing in vivo, but only weakly compared to the phage λ Redβ protein, highlighting putatively that Sak4 requires partners to be ...binds ...
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The Prion Protein Has RNA Binding and Chaperoning Properties Characteristic of Nucleocapsid Protein NCp7 of HIV-1
... minus strand Tar DNA (Tar⫺) and either PrP or HIV-1 NCp7 were performed for 10 min at 37 °C in 10 l of a buffer containing 20 m M Tris䡠HCl, pH ...molar protein to nt ...
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Post-transcriptional regulation during spermatogenesis : Role of the RNA-Binding Protein HuR
... detail, we analyzed the morphology of testis and epididymis in pre-pubertal and adult HuR tg mice. While progression of spermatogonial and meiotic stages is normal in HuR tg seminiferous tubules, impaired germ cell ...
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Régulation de l'apoptose des lymphocytes T par GIMAP5 (GTPase of Immune Associated Nucleotide Binding Protein 5)
... nucleotide-binding protein 5) gene, resulting in a truncated protein lacking 223 amino acids at the C-terminus that are replaced by 19 other residues ...overexpressed protein with intact ...
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Role of specific CCAAT/enhancer-binding protein isoforms in intestinal epithelial cells
... Here we show that C/EBP isoforms are involved in the regulation of acute phase protein gene haptoglobin and confirm the importance of these transcription factors in the control[r] ...
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12- to 22-membered bridged b-lactams as potential penicillin-binding protein inhibitors
... Val417 Ser418 Figure 6. Bottom of R39 cavity. The fragment in red is a part of the ligand bearing a side-chain on C(3) Three models have been built by increase of their complexity in order to locate the transition ...
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miR-25 Promotes Melanoma Progression by regulating RNA binding motif protein 47
... RNA binding motif protein 47 Matured miRNAs are single-stranded RNAs and act through a perfect or near perfect base pairing with the target mRNA 3'-untranslated regions (3'-UTRs), leading to ...
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DNA Methylation and Chromatin: Role(s) of Methyl-CpG-Binding Protein ZBTB38
... 22. Sasai N, Matsuda E, Sarashina E, Ishida Y, Kawaichi M. Identification of a novel BTB-zinc finger transcriptional repressor, CIBZ, that interacts with CtBP corepressor. Genes Cells. 2005;10:871–885. 23. Buck-Koehntop ...
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The RNA-Binding Protein RBPMS2 Regulates Development of Gastrointestinal Smooth Muscle
... Besides transcription, which represents the first step of gene expression, many post-transcriptional events regu- late the final fate of messenger RNAs (mRNAs) in eukary- otic cells and determine their spatiotemporal ...
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Bound or free: interaction of the C-terminal domain of Escherichia coli single-stranded DNA-binding protein (SSB) with the tetrameric core of SSB.
... for binding to SSB-binding proteins even in the absence of ...that binding of ssDNA to SSB is not a prerequisite for making the C-peptide available for binding to SSB-binding ...that ...
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Plasmid replication-associated single-strand-specific methyltransferases
... of single strands, in the context SAY (where S = C or G and Y = C or ...DNA strand, and is incomplete: typically, around 40% of susceptible motifs are ...gle strand in the recipient, early in ...
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Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species
... This study demonstrated that an engineered TbpB antigen is able to display epitopes from TbpA and provide stable, immunogenic antigens that can produce anti-TbpA antibodies that are bactericidal and able to inhibit ...
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Mitochondrion-dependent N-terminal processing of outer membrane Mcl-1 protein removes an essential Mule/Lasu1 protein-binding site
... RESULTS AND DISCUSSION To investigate Mcl-1 expression, we utilized SV40-trans- formed MEFs derived from WT and Mcl-1 ⫺ /⫺ mice (30). High resolution gel electrophoresis and combined immunoprecipi- tation and immunoblot ...
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Fluorescence-based nanofluidic biosensor platform for real-time measurement of protein binding kinetics
... dedicated protein-protein interaction ...streptavidin-biotin binding, we have confirmed that the grafting density of the receptors on the sensor surface played a crucial role in kinetic reactions and ...
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Characterization of an Enterococcus Hirae Penicillin-Binding Protein 3 with Low Penicillin Affinity
... Enterococcus hirae S185, a clinical isolate from swine intestine, exhibits a relatively high resistance to penicillin and contains two 77-kDa penicillin-binding proteins 3 of high (PBP 3[r] ...
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Caractérisation biochimique et implication dans la réponse au stress de la protéine "Selenium-Binding Protein" (SBP1) chez Arabidopsis thaliana
... Tableau 7 : Amorces utilisées pour la mutation silencieuse de la partie de la séquence codante de SBP1 reconnue par le micro ARN artificiel. Vecteurs codant pour l’expression des protéines GFP-SBP1 et SBP1-GFP La ...
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Age and albumin D site-binding protein control tissue plasminogen activator levels: neurotoxic impact.
... same fixative, rinsed in veronal buffer containing 20% sucrose and frozen in Tissue-Tek (Miles Scientific, Naperville, IL). Coronal sections of 10 mm were incubated overnight at room temperature with a primary antibody ...
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