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Serum-free culture medium

Serum-free culture medium and IL-7 costimulation increase the sensitivity of ELISpot detection.

Serum-free culture medium and IL-7 costimulation increase the sensitivity of ELISpot detection.

... Incorporating both of these parameters into the ELISpot procedure proved capable of greatly amplifying 35.1-fold increase the low grade CD8+ T cell responses directed against -cell epito[r] ...

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The New Serum-Free OptiPASS® Medium in Cold and Oxygen-Free Conditions: An Innovative Conservation Method for the Preservation of MDA-MB-231 Triple Negative Breast Cancer Spheroids

The New Serum-Free OptiPASS® Medium in Cold and Oxygen-Free Conditions: An Innovative Conservation Method for the Preservation of MDA-MB-231 Triple Negative Breast Cancer Spheroids

... 3D culture formation have enabled scientists to replicate models of avascular tumor in high number with acceptable reproducibility applicable for scalable and large range preclinical ...the culture ...

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Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)

Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)

... a serum-free medium: the basal defined medium (BDM) [41] supplemented with a mixture of non-essential amino acids and growth factors ...Eagle Medium (DMEM) containing glucose ...calf ...

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A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays

A defined synthetic substrate for serum-free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays

... fined serum-free medium or medium supplemented with 20% fetal bovine serum (FBS) for 1 ...in serum-free medium before processing for immunostaining against the ...

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Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches

Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches

... HS-supplemented medium resulted in a rate of differentiation comparable to that observed in hMuS- tem cells HS , indicating that the reduction in myogenic commitment capabilities caused by heparin-containing hPL ...

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The New Synthetic Serum-Free Medium OptiPASS Promotes High Proliferation and Drug Efficacy Prediction on Spheroids from MDA-MB-231 and SUM1315 Triple-Negative Breast Cancer Cell Lines

The New Synthetic Serum-Free Medium OptiPASS Promotes High Proliferation and Drug Efficacy Prediction on Spheroids from MDA-MB-231 and SUM1315 Triple-Negative Breast Cancer Cell Lines

... of culture and normalized to cell counting at day one, n = 9, bars = ...reference medium or OptiPASS medium were harvested and counted with a blue trypan exclusion ...supernatant medium, and ...

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Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells

Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells

... 50 /mL at the peak of production, the equivalent of more than 4000 doses of vaccine per litre. The very promising results generated in this feasibility study provide the bases for further process intensification work to ...

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Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells

Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells

... was left in the incubator at 37C for 30-60 minutes. Meanwhile, the pATX.V2.B6.Full plasmid along with the helper plasmids were mixed at different ratios (pATX.V2.B6.Full and pCAGGS T7 from 2 to 2.5 g/well, pBS-N from ...

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Culture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens.

Culture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens.

... standard medium for the sporulation of Fusarium species, it was used as a control to compare the sporulation between the species ( Leslie and Summerell, 2006 ...PDA culture, 230 rpm and incubated at 25 °C ...

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Role of the culture medium in porin expression and piperacillin-tazobactam susceptibility in Escherichia coli

Role of the culture medium in porin expression and piperacillin-tazobactam susceptibility in Escherichia coli

... containing medium that may differ in nutrients from the Mueller– Hinton (MH) reference medium (Livermore et ...the medium used for bacterial growth can play an important role by modulating the ...

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Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

... Bailey, M.J., Biely, P. & Poutanen, K. 1992 Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotech nology 3, 286 290. Chen, K.C., Lee, T.C. & Houng, J.Y. 1992 Search method for the ...

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Effect of culture medium on bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608

Effect of culture medium on bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608

... Biomass production Bacteria growth was measured in triplicate batch cultures for all strains in the different media, monitored by measuring the absorbance at 660 nm with a Genesis 200 spectrophotometer (Termo ...

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Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

... by a Fisher F test. Differentiation calculation was then employed for predicting the optimum point. Results and Discussion The ®rst step in the process of seeking optimum condi- tions is to identify the input variables ...

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A selective culture medium for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa

A selective culture medium for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa

... RESULTS A total of 92 isolates of worldwide origin were included in this study to evaluate the performance of the SuperCAZ/AVI medium. The ␤-lactamase contents of all strains were characterized at the molecular ...

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Enhanced culture recovery of Campylobacter with modified Cary-Blair medium: A practical field experience

Enhanced culture recovery of Campylobacter with modified Cary-Blair medium: A practical field experience

... Received date: 22 January 2018 Revised date: 1 May 2018 Accepted date: 1 May 2018 Please cite this article as: Clémence Massip, Hélène Guet-Revillet, Marion Grare, Agnès Sommet, Damien Dubois , Enhanced culture ...

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PCBs, PBDEs AND THEIR HYDROXYLATED METABOLITES IN SERUM OF FREE-RANGING HARBOUR SEALS (PHOCA VITULINA): LEVELS AND PROFILES

PCBs, PBDEs AND THEIR HYDROXYLATED METABOLITES IN SERUM OF FREE-RANGING HARBOUR SEALS (PHOCA VITULINA): LEVELS AND PROFILES

... Quality Assurance/Quality Control (QA/QC). Multi-level calibration curves (r 2 > 0.999) in the linear response interval of the detector were created for the quantification. QC was performed by regular analyses of ...

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Engagement dans la contre-culture free party et processus de personnalisation : les parcours singuliers des membres d'un sound system

Engagement dans la contre-culture free party et processus de personnalisation : les parcours singuliers des membres d'un sound system

... expériences Free Party avec une perspective développementale, nous retrouvons de nombreuses convergences entre les stratégies par lesquelles l’adolescent tente de surmonter certaines problématiques et les ...

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A penalization method to treat the interface between a free-fluid region and a fibrous porous medium

A penalization method to treat the interface between a free-fluid region and a fibrous porous medium

... the free fluid the porosity is, of course, unitary and the effective permeability ...porous medium far from the interface the porosity is constant and set equal to ...

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A penalization method to treat the interface between a free-fluid region and a fibrous porous medium

A penalization method to treat the interface between a free-fluid region and a fibrous porous medium

... for DNS or experimental data to be properly fit. In our case, this degree of freedom is represented by the choice of a linear trend for the components of the apparent permeability. The proposed approach has been tested ...

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Serum Creatinine: Not So Simple!

Serum Creatinine: Not So Simple!

... for Serum Creatinine As there are age and gender differences in creatinine generation, the determination of population-based nor- mal reference intervals has been the subject of detailed studies by Pottel et ...

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