protein to protein interaction
Genetic Algorithm to Detect Different Sizes’ Communities from Protein-Protein Interaction Networks
... Biology to Sociology and Computer ...the Protein-protein or Gene-gene Interaction (PPI) ...represent protein-protein or gene-gene interactions which corresponds to a set ...
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Cell surface protein-protein interaction analysis with combined time-resolved FRET and snap-tag technologies: application to GPCR oligomerization
... used to examine FRET at the plasma membrane, such approaches are not compatible with systematic and quantitative assessments of the ...the protein proximity at the cell surface enables a clear demonstration ...
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In cellulo protein-mRNA interaction assay to determine the action of G-quadruplex-binding molecules
... out to detect the NCL-EBNA1 mRNA interactions in situ using either transfected cells or EBV-infected ...an interaction between NCL and EBNA1 ...indeed, protein-mRNA interactions control pivotal steps ...
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A computational tool to predict the evolutionarily conserved protein-protein interaction hot-spot residues from the structure of the unbound protein
... expected to contribute greatly to protein-protein binding ...energy. To identify the exposed hydrophobic residues, the SIM tool uses the normalized (with respect to its ...
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Protein-lipid Interaction Analysis by Surface Plasmon Resonance (SPR)
... several cycles of analyte injection and regeneration in order to evaluate ligand performance (responses obtained from the binding assays should not vary more than 10% of the response of the first injection). Note: ...
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The nanovirus-encoded Clink protein affects plant cell cycle regulation through interaction with the retinoblastoma-related protein.
... transferred to new plates containing 30 M Dex or control plates containing ...mut protein in leaves was con- firmed by Western blot analysis using Clink-specific antibodies ...compared to wild-type ...
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Protein-Protein Interaction in Purple Membrane
... a protein crystal (from computer simulations [ 4 , 5 ]) and hydrated protein powder (from inelastic x-ray scattering [ 6 ]), and energies of about 1 meV for inter-, and even 10 meV for intraprotein motions ...
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Binder of SPerm protein interference in sperm-egg interaction
... suggested to have a chaperone-like activity, to be a cell viability marker as well as to be involved in the formation of the oviductal sperm reservoir and, more recently, in sperm-egg ...
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A Cell-Type-Specific Protein-Protein Interaction Modulates Transcriptional Activity of a Master Regulator in Caulobacter crescentus
... (EMSAs) to test whether SciP could interact with CtrA when CtrA is bound to target ...CtrAP to radiolabeled, 50 base pair fragments of the ccrM, pilA, and fliF promoters, which contain CtrA-binding ...
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JET2 Viewer: a database of predicted multiple, possibly overlapping, protein–protein interaction sites for PDB structures
... criterion to distinguish biologically relevant in- terfaces from lattice contacts in protein ...unit. To cope with this issue in the evaluation, binding sites sharing more than 80% of their residues ...
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Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study
... processes, protein flexibility is a fundamental property allowing protein-protein ...combined to EPR spectroscopy is a powerful technique to follow structural modifications within ...
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Genome-wide identification, phylogenetic analysis, expression profiling, and protein-protein interaction properties of TOPLESS gene family members in tomato
... discriminating interaction features among various TPL ...order to phenocopy the dominant mutation tpl-1 ( Long et ...contrast to the situation prevailing in rice and maize, where genetic evidence ...
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Designing helical peptide inhibitors of protein–protein interactions
... affinities to train a scoring function for bZIP interaction ...used to design selective helical peptide inhibitors of bZIP proteins JUN, XBP-1, ATF4 and ...methods to generate peptide ...
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An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach
... sparse to provide any insight into the functional role of the ...Section). To obtain functional information on the whole PPI network and in particular on the proteins of the GoH, ...shown to increase ...
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Analysis of protein contacts into Protein Units.
... some protein domains are not sequential ...of Protein Peeling can be compared to protein domain assignment, ...parameters to define a contact [64]. Thus, a Protein Peeling based ...
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Extreme multifunctional proteins identified from a human protein interaction network.
... As candidates are defined with respect to their module’s annotations, their identification is dependent on the quality of these annotations. Said quality was assessed by performing three types of randomization ...
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Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology
... of protein surface that must be engaged to form a functional complex is in the order of 900 Å 2 (around 500 Å 2 provided by each partner) [ 3 ...in protein-protein pairing, the identification ...
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A non-parametric method to compute protein–protein and protein–ligands interfaces. Application to HIV-2 protease–inhibitors complexes
... RNA-protein interaction interfaces via the Voronoi ...descriptors to analyze interresidue contacts in protein structures and protein ...
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Global alignment of protein-protein interaction networks by graph matching methods.
... order to compare the different methods in different settings and to test different formulations of the GNA ...is to disambiguate functional orthologs within Inparanoid clusters using PPI ...out ...
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A New Protein-Protein Interaction Sensor Based on Tripartite Split-GFP Association
... Methods Cloning. K1-coil and E1-coil DNA, FRB (NM_004958) and FKBP (NM_000801) sequences were amplified by PCR using synthetic oligonucleotides (SI note) and cloned into a pTET ColE1 SpecR GFP10/11 via NdeI:KpnI (GFP10 ...
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