pDC: plasmacytoid dendritic cells
Classical swine fever virus npro limits type I interferon induction in plasmacytoid dendritic cells by interacting with interferon regulatory factor 7
... in pDC. (A) Enriched pDC were mock treated or infected at a MOI of 2 TCID50 /cell with CSFV strain vA187-1 or with CSFV vA187-1 carrying the C 112 R or the D 136 N mutation in N pro ...Enriched pDC ...
11
Endothelial cell-derived microparticles induce plasmacytoid dendritic cell maturation: potential implications in inflammatory diseases.
... between PDC and EMP in vitro have never been evaluated to date. Since PDC activation and endothelial damage associated with EMP production are encountered in similar diseases, we decided to explore these ...
12
Breast cancer-derived transforming growth factor-β and tumor necrosis factor-α compromise interferon-α production by tumor-associated plasmacytoid dendritic cells
... Furthermore, IF stainings showed that IRF-7 protein, local- ized in the cytoplasm of resting pDC, was upregulated and translocated in the nucleus after 3-hr stimulation with Flu virus (Fig. 3d). This process was ...
9
Dual Role of the Tyrosine Kinase Syk in Regulation of Toll-Like Receptor Signaling in Plasmacytoid Dendritic Cells
... of plasmacytoid dendritic cells (pDCs) efficiently suppresses production of type-I interferon (IFN)- α/β and other cytokines in response to Toll-like receptor (TLR) 7/9 ...the pDC cell line ...
12
Coxiella burnetii Induces Inflammatory Interferon-Like Signature in ă Plasmacytoid Dendritic Cells: A New Feature of Immune Response in Q ă Fever
... mononuclear cells (PBMCs) were recovered using density gradient ...flow-through. Plasmacytoid dendritic cells were then suspended in RPMI 1640, supplemented with 20 mM HEPES, 10% fetal calf ...
10
ICOS is associated with poor prognosis in breast cancer as it promotes the amplification of immunosuppressive CD4+T cells by plasmacytoid dendritic cells
... ICOS increases the secretion of IL-2 in TA-CD4 + T cells/pDC co-cultures but only slightly reduces the secretion of IFNγ and the proliferation of Tconvs, and does not impact T-cell responses to mDCs. This ...
4
Plasmacytoid, conventional, and monocyte-derived dendritic cells undergo a profound and convergent genetic reprogramming during their maturation
... all conditions. Boxes highlight clusters of q_DC subsets (gray) versus a_DC subsets (black). (B) Principal component analysis on all Probe- Sets present on the microarrays. (C) DC subsets maintain their identity after ...
11
en fr Liver and peripheral toleranceRole of plasmacytoid dendritic cells and NKT cells Foie et tolérance périphériqueRôle des cellules dendritiques plasmacytoides et des cellules NKT
... populations de CD que les organes lymphoïdes secondaires : des CD lymphoïdes CD11b - CD8α + , des CD myéloïdes CD11b + CD8α - et des pDC, ainsi qu’une population atypique exprimant des marqueurs de cellules NK ...
253
Peripheral Blood Plasmacytoid Dendritic Cells at Day 100 Can Predict Outcome after Allogeneic Stem Cell Transplantation
... host cells. Den- dritic cells (DCs) are key antigen-presenting cells that present host antigens to donor T ...subsets, plasmacytoid DCs (pDCs) have been proposed to play a major role in ...
7
Virosome-mediated delivery of tumor antigen to plasmacytoid dendritic cells.
... targeting plasmacytoid dendritic cells ...a PDC line developed in our laboratory, we evaluated the response of Melan-A specific T ...bind PDC and are endocyted within vesicles in the ...
21
Production of large numbers of plasmacytoid dendritic cells with functional activities from CD34+ hematopoietic progenitor cells: use of IL-3
... full pDC matured phenotype was obtained with an IL-3 concentration of 20 ng/mL in ...for pDC generation or shorten the time frame needed for successful pDC ...more pDC at days 14 and 21 of ...
13
en fr Plasmacytoid dendritic cells and viral infections : physiopathologic role and vaccinal potency Cellules dendritiques plasmocytoïdes et infections virales : rôle physiopathologique et potentiel vaccinal
... de pDC a induit une plus grande amplification de lymphocytes T spécifiques de l’antigène HBc que l’utilisation du peptide HBc seul ex vivo, et une stimulation immunitaire plus rapide que lors de l’utilisation de ...
148
Zika Virus Inhibits IFN-a Response by Human Plasmacytoid Dendritic Cells and Induces NS1-Dependent Triggering of CD303 (BDCA-2) Signaling
... exploring pDC activation by flaviviruses have revealed that sensing of virus-infected cells by pDCs was more effective than sensing of circulating cell-free ...of pDC-mediated antiviral state, ...
15
Plasmacytoid dendritic cells deficient in IFNα production promote the amplification of FOXP3+regulatory T cells and are associated with poor prognosis in breast cancer patients
... HD-derived pDC precon- ditioned with BT supernatants, indicating that the induction of tolerance is strongly amplified in tumors as a result of impaired IFNα production by ...
4
Molecular dissection of plasmacytoid dendritic cell activation in vivo during a viral infection
... that pDC production of pro-inflammatory cytokines is mainly IRF5-dependent (Takaoka et al, ...mouse pDC has been associated with signaling from different endosomes (Sasai et al, ...most cells, IRF7 ...
20
Plasmacytoid dendritic cells capture and cross-present viral antigens from influenza-virus exposed cells.
... influenza-treated cells Because PDC are involved in anti-viral immune responses, we sought to determine whether GEN3 cells capture cellular antigens from virus-infected ...B cells incubated ...
11
Arsenic trioxide induces regulatory functions of plasmacytoid dendritic cells through interferon-α inhibition
... 2.2. pDC isolation and culture Peripheral blood mononuclear cells (PBMCs) were obtained from buffy coats by Ficoll density centrifugation, from healthy donors (Etablissement Franc¸ais du Sang, Paris ...
13
en fr Characterization of tumor associated alterations of myeloid and plasmacytoid dendritic cells in a mouse mammary tumor model Caractérisation des altérations fonctionnelles des cellules dendritiques plasmacytoïdes et myéloïdes dans les tumeurs mammaires murines
... of pDC ability to produce IFN-α was mainly mediated through tumor-derived TGF-β and ...inducing pDC maturation (Palucka et ...enhance pDC maturation (data not shown) potentially through TNF-α thus ...
225
Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis
... Plasmacytoid Dendritic Cell Accumulation in In flamed Skin Is Severely Inhibited in Zdhhc2 De ficient Mice As we found marked decrease of IFN-a in psoriatic skin, the level of which was regarded as hallmark ...
12
TLR7 stimulation in human plasmacytoid dendritic cells leads to the induction of early IFN-inducible genes in the absence of type I IFN.
... human pDC activation via TLR7 This study focuses on TLR7 signaling in ...human pDC cell line, GEN2.2, was used as a model of pDC 13,14 ...GEN2.2 cells (Figure ...GEN2.2 cells was ...
34