Non viral gene delivery
Peptides derived from the C-terminal domain of HIV-1 Viral Protein R in lipid bilayers: Structure, membrane positioning and gene delivery
... Abstract Viral protein R (Vpr) is a small accessory protein of 96 amino acids that is present in Human and simian immunodeficiency ...the gene delivery ...
38
Transferrin Non-Viral Gene Therapy for Treatment of Retinal Degeneration
... TF non-viral gene therapy based on the electrotransfection of pEYS611, a plasmid encoding human TF, into the ciliary muscle was evaluated in several rat models of retinal ...TF ...
23
PEGylated quaternized copolymer/DNA complexes for gene delivery
... Davis, M.E., 2002. Non-viral gene delivery systems. Curr. Opin. Biotechnol. 13, 128-131. De Smedt, S.C., Demeester, J., Hennink, W.E., 2000. Cationic polymer based gene delivery ...
13
Degradable Terpolymers with Alkyl Side Chains Demonstrate Enhanced Gene Delivery Potency and Nanoparticle Stability
... Nanoparticle; Gene therapy Non-viral gene delivery vectors are attractive due to their potential safety and ease of manufacture, but low transfection potency and poor formulation ...
13
Noninvasive gene delivery to foveal cones for vision restoration
... of gene therapies aiming to treat inherited retinal diseases like midstage retinitis pigmentosa (4, 5) and achromatopsia ...Currently, viral vectors encoding therapeutic proteins need to be injected into ...
19
Chloroquine-enhanced gene delivery mediated by carbon nanotubes
... efficient gene expression is intracellular trafficking of the internalized vec- tor [7] ...the gene expression machinery (for RNA) in the cytoplasm. However, most non- viral vectors, including ...
12
Gene delivery to pancreatic exocrine cells in vivo and in vitro
... of viral vectors (5 × 10 9 Pfu) in a small in- jection volume (200 μl), mesenchymal cells between pancreatic lobes and peripherally located epithelial cells were transduced, whereas with a higher volume (≥ 400 μl) ...
13
Chemical modification of the adeno-associated virus capsid to improve gene delivery
... the viral surface are most commonly exploited as a molecular anchor for conjugating amine-reactive ...of gene expression upon re-administration without compromising the patient's immune ...
10
Biomaterials for non-viral delivery of nucleic acid drugs
... deliver non-viral macromolecular therapeutics in ...drug delivery vehicles conjugated with targeting peptides specific for cancer cells that also entrapped active siRNA gene effector ...465 ...
97
Ultrasound and microbubble-assisted gene delivery: insights for intracellular mechanism
... microbubble-assisted gene delivery: insights for intracellular mechanism Introduction Gene delivery by non-viral methods is still a major challenge ...for gene therapy ...
20
Cyclodextrins in drug delivery: applications in gene and combination therapy
... attractive non-viral delivery vectors because they are highly customizable, both in size and type of genetic material used for delivery – this stems in part from the fact that cationic ...
34
A Dual Targeting Dendrimer-Mediated siRNA Delivery System for Effective Gene Silencing in Cancer Therapy
... Both viral and nonviral delivery vectors have been explored for siRNA ...Although viral delivery is more e ffective, increasing concerns over the immunogenicity and toxicity of viral ...
12
en fr NON-VIRAL VECTORIZATION OF THERAPEUTIC MOLECULES FOR LUNG CANCER THERAPY VECTORISATION NON-VIRALE DE MOLECULES THERAPEUTIQUES POUR LA THERAPIE DES CANCERS DU POUMON
... II. PEI-mediated siRNA Delivery in Vivo II.1. Introduction RNA interference (RNAi) is an evolutionarily conserved response to double- stranded RNA (dsRNA) that leads to sequence specific post-transcriptional ...
295
Emerging technologies for delivery of biotherapeutics and gene therapy across the blood–brain barrier
... and gene therapies developed for neurological indications face a delivery challenge posed by various anatomical and physiological barriers within the central nervous system (CNS); most notably, the ...
14
Vectors and Gene Delivery to the Retina
... 2.3. Viral Vectors The retina has several characteristics that make it a favorable tissue for gene ...for gene delivery to the neural retina, AAV is the only vector that can be used via ...
21
Systemic gene delivery to the central nervous system using Adeno-associated virus
... efficient gene delivery and brain transduction has been reported after systemic injection of rAAV in macaque monkeys ( Bevan et ...both non-human primate and humans ( Boutin et ...of viral ...
9
Ultrasound and microbubble-assisted gene delivery in Achilles tendons: long lasting gene expression and restoration of fibromodulin KO phenotype
... fibromodulin gene in fibromodulin KO ...this gene delivery method is promising for clinical applications aimed at modulating healing or restoring a degenerative tendon while offering great promise for ...
9
Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery
... 4.4. Constant number of interaction sites The experimental results show that after the first interaction sites become visible, no further sites appear during subsequent pulsation. An explanation for this is that the ...
19
Non-neutralizing IgG anti-PID antibodies decreased viral load following high dose vaginal challenge of non-human primates
... L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ...
2
Revisiting Cationic Phosphorus Dendrimers as a Nonviral Vector for Optimized Gene Delivery Toward Cancer Therapy Applications
... flow cytometry and fluorescence microscopic imaging analyses. Hence, 1-G1 dendrimers were then used as a vector to transfect pDNA encoding both EGFP and p53 protein for cancer gene therapy applications. Our ...
22