Nanopore sequencing
De novo assembly of a new Solanum pennellii accession using nanopore sequencing
... Oxford nanopore has emerged as a competitor for long-read ...Oxford nanopore produces a mini- sequencer, the MinION, requiring only a start-up fee of $1000, which includes two flow cells and a library ...
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Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries
... ’ sequencing). Nanopore sequencing has been applied to genomic [3], meta genomic [4], transcriptomic [5] and HLA typing [6] ...to sequencing of single-domain Ab (sdAb) repertoires and ...
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FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing
... MinION nanopore sequencing (Oxford Nanopore Technologies, ONT) has the poten- tial to bypass these ...a nanopore through which DNA is translocated. Importantly, the nanopore sequencer ...
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CarrierSeq: a sequence analysis workflow for low-input nanopore sequencing
... carrier sequencing, a method to sequence low-input DNA by preparing the target DNA with a genomic carrier to achieve ideal library preparation and sequencing stoichiometry without ...by sequencing ...
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Transcriptome profiling of mouse samples using nanopore sequencing of cDNA and RNA molecules
... Illumina sequencing of ONT library preparation or Lexogen TeloPrime ...ONT sequencing is promising for studying RNA, especially if the number of reads and full-length reads continues to ...deep ...
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A framework for in situ molecular characterization of coral holobionts using nanopore sequencing
... and sequencing. Sequencing libraries were prepared for ...PCR Sequencing Kit SQK-LWP001 according to the four-primers PCR protocol from ...ligation sequencing kit SQK-LSK109, which is ...
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DECODING OF NANOPORE-SEQUENCED SYNTHETIC DNA STORING DIGITAL IMAGES
... Index Terms— DNA data storage, Nanopore sequencing, image coding, error detection, consensus finding 1. INTRODUCTION The exponential growth of digital information threatens to ex- ceed the capacity of ...
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High throughput error corrected Nanopore single cell transcriptome sequencing
... A t-SNE plot of the Illumina short-read gene expression data reveals typical cell types for E18 mouse brain (Fig. 2 d). t-SNE projection of transcript isoform expression defined by Nanopore sequencing (Fig. ...
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Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
... the nanopore during the translocation ...biological nanopore using an unfoldase molecular motor (ClpX variant) 27 ...biological nanopore was too fast to read the protein sequence 18 , 48 ...the ...
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Load sequencing for double-stack trains
... load sequencing for double-stack ...load sequencing scale well for realistic in- stance size, managing to achieve a significantly reduced computing time with a small loss in solution quality compared to ...
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Nanoparticle Electrical Analysis and Detection with a Solid-state Nanopore in a Microfluidic Device
... c C2N UMR9001, Marcoussis 91460, France Abstract Our society is increasingly exposed to harmful nano and nanobio particles as new viruses, harmful proteins (like prions) and metal- lic nanoparticles that need to be ...
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Kinetic Analysis of Single Molecule Electrodiffusion in a Biological Nanopore with Two Binding Sites
... Kinetic Analysis of Single Molecule Electrodiffusion in a Biological Nanopore with Two Binding Sites.. Norbert Ankri, Mordjane Boukhet, Gerhard Baaken, Murugappan Muthukumar, Jan Behrend[r] ...
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High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.
... generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in a variety of cellular processes shaping the bacterial ...ChIP- sequencing (ChIP-seq), enables the ...
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Report on sequencing the wheat chromosome 3B
... Sequencing and Analyses of the Hexaploid Wheat Chromosome 3B Frédéric Choulet, Patrick Wincker, Hadi Quesneville, Dominique Brunel, Bikram S. Gill, Rudi Appels, Beat Keller, Catherine Feuillet INRA Université ...
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Task sequencing for sensor-based control
... all the degrees of freedom of a robot during the execution of a task. In this article a new solution is proposed. The key idea is to divide the global full-constraining task into several subtasks that can be applied or ...
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Transport of Long Neutral Polymers in the Semidilute Regime through a Protein Nanopore
... Our experiments open the way to new studies, in semi- dilute solution, of the dynamics of macromolecules or biomolecules in confined geometry. The entry of small chains is possible when their radius of gyration is ...
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Single-sulfur atom discrimination of polysulfides with a protein nanopore for improved batteries
... the nanopore approach to detect and dis- criminate at the single molecule level each ...the nanopore, or clogging the nanopore without translocating, partially block ion transport inducing a ...
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Selective Target Protein Detection Using a Decorated Nanopore into a Microfluidic Device
... large nanopore, we assume that each streptav- idin is bound to at least two ...biotinylated nanopore can specifically capture streptavidin (around 48/4 = 12 streptavidin molecules in the 20 nm large ...
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Ionic transport through a protein nanopore: a Coarse-Grained Molecular Dynamics Study
... nanopore: a coarse-Grained Molecular Dynamics Study nathalie Basdevant , Delphine Dessaux & Rosa Ramirez * The MARTINI coarse-grained (CG) force field is used to test the ability of CG models to simulate ...
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Nanopore DNA sequencing channel modeling
... the output nanopore sequencers. This leads to a new DNA sequencer channel model that opens new directions in coding strategies for DNA data storage. The contributions of this paper can be listed as: first, we ...
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