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NADPH-cytochrome P450 reductase (CPR)

The Hinge Segment of Human NADPH-Cytochrome P450 Reductase in Conformational Switching: The Critical Role of Ionic Strength

The Hinge Segment of Human NADPH-Cytochrome P450 Reductase in Conformational Switching: The Critical Role of Ionic Strength

... France NADPH-cytochrome P450 reductase (CPR) is a redox partner of microsomal cytochromes P450 and is a prototype of the diflavin reductase ...of cytochrome c at various ...

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Probing the role of the hinge segment of cytochrome P450 oxidoreductase in the interaction with cytochrome P450

Probing the role of the hinge segment of cytochrome P450 oxidoreductase in the interaction with cytochrome P450

... Abstract: NADPH-cytochrome P450 reductase (CPR) is the unique redox partner of microsomal cytochrome P450s ...and cytochrome b5 (CYB5) on CYP ...

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Immobilization of cytochrome P450 BM3 from Bacillus megaterium on magnetic nanoparticles to develop an effective biocatalyst for hydroxylation reactions

Immobilization of cytochrome P450 BM3 from Bacillus megaterium on magnetic nanoparticles to develop an effective biocatalyst for hydroxylation reactions

... reaction, P450 inserts one atom from dioxygen into the substrate while reducing the other oxygen atom to a water molecule is accomplished thanks to electrons received from the nicotinamide cofactor NADPH ...

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Unexpected rapid aerobic transformation of 2,2,6,6-tetraethyl-4-oxo (piperidin-1-yloxyl) radical by cytochrome P450 in the presence of NADPH: Evidence against a simple reduction of the nitroxide moiety to the hydroxylamine

Unexpected rapid aerobic transformation of 2,2,6,6-tetraethyl-4-oxo (piperidin-1-yloxyl) radical by cytochrome P450 in the presence of NADPH: Evidence against a simple reduction of the nitroxide moiety to the hydroxylamine

... [39]. Cytochrome P450 contents were determined by the method of Omura and Sato ...The cytochrome c reductase activity was assayed using the protocol of Vermilion and Coon [41] and corresponded ...

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Characterization of a cytochrome P450 monooxygenase gene involved in the biosynthesis of geosmin in Penicillium expansum

Characterization of a cytochrome P450 monooxygenase gene involved in the biosynthesis of geosmin in Penicillium expansum

... Screening of the transformants Hygromycin-resistant transformants were selected on YES medium (20 g/L of yeast extract, 1 M sucrose, 15 g/L of agar) supplemented with 150 µg/mL of hygromycin B. Transformant plates were ...

7

Characterization of a cytochrome P450 monooxygenase gene involved in the biosynthesis of geosmin in Penicillium expansum

Characterization of a cytochrome P450 monooxygenase gene involved in the biosynthesis of geosmin in Penicillium expansum

... like cytochrome P450 monooxygenases, as has been suggested in bacteria (Lamb et ...fragments, p450-1 and p450-2, corresponding to parts of putative cytochrome P450 monooxygenase ...

8

Influence du chondroïtine sulfate (CS) 
sur l’activité et l’expression de plusieurs isoformes du cytochrome P450 
et de la NADPH P450 réductase

Influence du chondroïtine sulfate (CS) sur l’activité et l’expression de plusieurs isoformes du cytochrome P450 et de la NADPH P450 réductase

... the cytochrome P450 (Letarte & du Souich, 1984; Wright & Morgan, 1990; Parent et ...of cytochrome P450 genes impeding the transcription of the gene (Iber et ...

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Synthèse de ligands base de schiff non symétriques et obtention de complexes de métaux de transition propriétés électrochimiques appliquées au modèle cytochrome p450

Synthèse de ligands base de schiff non symétriques et obtention de complexes de métaux de transition propriétés électrochimiques appliquées au modèle cytochrome p450

... I.4.2. Systèmes catalytiques utilisant l'oxygène moléculaire comme source d'atomes d'oxygène Les systèmes catalytiques utilisant l'oxygène moléculaire présentent l'avantage de se rapprocher des conditions réelles des ...

157

In vitro assay for ABA 8'-hydroxylase: implications for improved assays for cytochrome P450 enzymes

In vitro assay for ABA 8'-hydroxylase: implications for improved assays for cytochrome P450 enzymes

... Methods Cytochrome P450 enzymes are typically associated with the endoplasmic reticulum (ER) so successful development of an authentic in vitro assay requires preparation of a microsomal fraction enriched ...

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Four Major Channels Detected in the Cytochrome P450 3A4: A Step toward Understanding Its Multispecificity

Four Major Channels Detected in the Cytochrome P450 3A4: A Step toward Understanding Its Multispecificity

... B-C block and β 4 sheet (in 3A4, it is the C-terminal loop, as denoted further in the text; size slightly depending on the conformations of a given isoform), and F-G block (helix-loop-helix), very flexible due to channel ...

23

Gene Duplication Leads to Altered Membrane Topology of a Cytochrome P450 Enzyme in Seed Plants

Gene Duplication Leads to Altered Membrane Topology of a Cytochrome P450 Enzyme in Seed Plants

... Spatial Arrangements Calculations by OPM Database The PDB coordinates of model CYP73 N-terminal segments were submitted to PPM server (http://opm.phar.umich.edu/ server.php; last accessed May 22, 2017) for calculating ...

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Pépite | Etude de la relation entre le polymorphisme génétique du cytochrome P450 2D6 et la réponse clinique au citrate de clomiphène chez des femmes infertiles dysovulantes

Pépite | Etude de la relation entre le polymorphisme génétique du cytochrome P450 2D6 et la réponse clinique au citrate de clomiphène chez des femmes infertiles dysovulantes

... Contexte : Le cytochrome P450 2D6 participe au métabolisme de nombreux médicaments. Il présente un polymorphisme génétique responsable de quatre phénotypes de métabolisme différents. Le génotype de cette ...

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Optimisation des conditions réactionnelles et création de nouveaux mutants à grande performance du cytochrome p450 BM3 CYP102A1 utilisant les cofacteurs alternatifs NADH et N-benzyl-1,4-dihydronicotinamide

Optimisation des conditions réactionnelles et création de nouveaux mutants à grande performance du cytochrome p450 BM3 CYP102A1 utilisant les cofacteurs alternatifs NADH et N-benzyl-1,4-dihydronicotinamide

... for cytochrome p450 enzymes ...name p450 when, by chance, it was discovered that bubbling carbon monoxide (CO) unto the microsome preparation resulted in an increase in absorbance which peaked at 450 ...

240

Cytochrome P450 2E1 should not be neglected for acetaminophen-induced liver injury in metabolic diseases with altered insulin levels or glucose homeostasis

Cytochrome P450 2E1 should not be neglected for acetaminophen-induced liver injury in metabolic diseases with altered insulin levels or glucose homeostasis

... Acetaminophen (APAP) hepatotoxicity is mediated by N-acetyl-p-benzoquinone imine (NAPQI), a highly toxic metabolite generated by cytochrome P450 2E1 (CYP2E1). Thus, pathological conditions increasing CYP2E1 ...

19

The Role of the FMN-Domain of Human Cytochrome P450 Oxidoreductase in Its Promiscuous Interactions With Structurally Diverse Redox Partners

The Role of the FMN-Domain of Human Cytochrome P450 Oxidoreductase in Its Promiscuous Interactions With Structurally Diverse Redox Partners

... doi: 10.3389/fphar.2020.00299 Edited by: Miia Turpeinen, Oulu University Hospital, Finland Reviewed by: Ayyalusamy Ramamoorthy, University of Michigan, United States Evgeniy Yablokov, Russian Academy of Medical Sciences ...

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Modélisation moléculaire de la protéine "Steroidogenic Acute Regulatory" (StAR) et du cytochrome P450 17[alpha]-hydroxylase/17,20-Lyase (P450c17) une approche moléculaire pour comprendre les mécanismes de la stéroïdogenèse

Modélisation moléculaire de la protéine "Steroidogenic Acute Regulatory" (StAR) et du cytochrome P450 17[alpha]-hydroxylase/17,20-Lyase (P450c17) une approche moléculaire pour comprendre les mécanismes de la stéroïdogenèse

... At this time, we have been unable to identify single AA substitutions that account for the difference in activities of the P450c 17 enzymes from various species such as the [r] ...

334

Synergism between oral estrogen therapy and cytochrome P450 3A5*1 allele on the risk of venous thromboembolism among postmenopausal women.

Synergism between oral estrogen therapy and cytochrome P450 3A5*1 allele on the risk of venous thromboembolism among postmenopausal women.

... The impact of the CYP3A5*3 SNP on the relation between oral estrogen use and VTE risk is biologically plausible. When administrated by oral route, estrogen therapy results in a sub- stantial increase in plasma estrone ...

7

Cytochrome P450 1A1/2, 2B6 and 3A4 HepaRG cell-based biosensors to monitor hepatocyte differentiation, drug metabolism and toxicity

Cytochrome P450 1A1/2, 2B6 and 3A4 HepaRG cell-based biosensors to monitor hepatocyte differentiation, drug metabolism and toxicity

... Keywords: HepaRG cells; cell-based biosensors; cytochromes P450; drug screening; hepatotoxicity 1. Introduction The liver plays a crucial role in the biotransformation and elimination of chemicals, pollutants and ...

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Cytochrome P450 dependent metabolism of fluindione in vitro in rat and human microsomes and in vivo in rat.

Cytochrome P450 dependent metabolism of fluindione in vitro in rat and human microsomes and in vivo in rat.

... Recombinant human P450: The formation of M2 was studied using insect cell microsomes expressing recombinant CYP1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5. CYP1A2, 2B6, 2C9, 2D6, 3A4 and 3A5 could make ...

3

Exposition prénatale aux sous-produits de chloration de l'eau, polymorphismes des gènes du cytochrome P450 et le risque de retard de croissance intra-utérin

Exposition prénatale aux sous-produits de chloration de l'eau, polymorphismes des gènes du cytochrome P450 et le risque de retard de croissance intra-utérin

... du cytochrome P450 de la phase I de biotransformation des xénobiotiques et effet de leurs polymorphismes sur la détoxification ...du cytochrome P450 : CYP1A2, CYP2A6 et CYP2D6 Les isoenzymes ...

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