Methyl-CpG-binding domain (MBD)
en fr The Methyl-CpG Binding Domain protein 2 (MBD2), a DNA methylation-dependent transcriptional repressor : identification and caracterization of MBD2 targets by genome-wide approach Étude systémique des cibles génomiques de la methyl-CpG binding domain protein 2 (MBD2), un répresseur transcriptionnel dépendant de la méthylation de l'ADN : évolution de la distribution de MBD2 dans un modèle syngénique de progression tumorale mammaire
... functional methyl-CpG- binding domain (MBD) responsible of their binding specificity ...lacks methyl-CpG-binding activity due to amino acid changes at critical ...
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en fr The Methyl-CpG-Binding Domain Protein 2 (MBD2) : a specific interpret of methylated loci in cancer cells Spécificité de liaison et de répression de la « Methyl-CpG-Binding Domain protein 2 » (MBD2) : identification de gènes cibles impliqués dans les cancers
... (Methyl-CpG-Binding Domain protein 2), a été montré comme possédant la capacité de catalyser in vitro comme in vivo l’hydrolyse du résidu méthyle des cytosines méthylées et ce uniquement en ...
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Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus
... The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene ...their binding specificity at the constitu- tively ...
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DNA Methylation and Chromatin: Role(s) of Methyl-CpG-Binding Protein ZBTB38
... BTB domain–containing protein 38) is one of these methyl-CpG-sensitive ...POZ/BTB domain (broad complex, tramtrack, bric-a-brac/pox zinc ...DNA binding domain(s) can accommodate ...
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Stabilization of the methyl-CpG binding protein ZBTB38 by the deubiquitinase USP9X limits the occurrence and toxicity of oxidative stress in human cells
... 1195 967 Figure 1. USP9X and ZBTB38 interact. (A) Silver staining of the purified HA-Flag-ZBTB38 complex. HeLa cells stably expressing an HA-Flag-ZBTB38 construct were lysed and the soluble nuclear fraction subjected to ...
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Mapping of the lipid-binding and stability properties of the central rod domain of human dystrophin.
... Cloning The plasmid pTG11025 harbouring the cDNA for the Dp427m muscle isoform of human dystrophin (NCBI Nucleotide Data base NM-004006, provided by S. Braun Transgene, France) was used as a template for PCR ...
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A critical regulatory role for the cytoplasmic domain of CD28 in ligand binding in naive T cells
... CD28 binding to its ...cytoplasmic domain (amino acids 163–198) but displays normal surface expression and binding to soluble recombinant B7-1–Fc [11] ...2D binding to B7-1, which was ...
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Biological and medical implications of discordance in CpG methylation
... In cancer, the clear methylation pattern observed in normal cells is eroded, so global methylation levels decrease, and methylation increases at previously-unmethylate[r] ...
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Co-production of butyrate methyl ester and triacetylglycerol from tributyrin and methyl acetate
... The increase in biodiesel (fatty acids methyl esters, FAME) pro- duction as renewable fuel has been accompanied by a concurrent increase in the availability of its main co-product, glycerol. The pro- cedure to ...
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Spectral editing of intra- and inter-chain methyl–methyl NOEs in protein complexes
... the methyl resonances could be unambiguously assigned by the soft- ware, five were left without assignment (three of those due to missing NOE cross-peaks), and the remaining ones were given multiple ...
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Potassium Acts as a GTPase-Activating Element on Each Nucleotide-Binding Domain of the Essential Bacillus subtilis EngA
... Discussion Many enzymes often require co-factors for an optimal functioning and monovalent cations, especially potassium, have long been known to be required for a wide variety of biochemical pathways [47]. Early on, ...
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Coordination of Hpr1 and Ubiquitin Binding by the UBA Domain of the mRNA Export Factor Mex67.
... Figure 2. Role of helix H4 of the Mex67 UBA domain in its interaction with Hpr1. (A) Pull- down assays using indicated GST fusion re- combinant proteins and lysates from cells ex- pressing HA-tagged versions of ...
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Frizzled 7 and PIP2 binding by syntenin PDZ2 domain supports Frizzled 7 trafficking and signalling
... given plus–minus s.d.’s. Liposome-binding experiments . The L1 chip (GE Healthcare) was coated with composite liposomes (30% PC/40% PE/20% PS/5% PI and 5% PIP 2 or 10% PI for background reference). Liposomes were ...
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Point mutations in BCL6 DNA-binding domain reveal distinct roles for the six zinc fingers.
... ZF domain, including ZFs 1 and ...specific binding to DNA while the two first were reported to mediate protein–protein interaction with PML [ 19 ...the binding to two unrelated DNA-target sequences, ...
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Identification and phylogenetic analyses of VASt, an uncharacterized protein domain associated with lipid-binding domains in Eukaryotes
... transport domain [PDB:1EM2] and Streptomyces ZhuI polyketide aromatase/cyclase ...VASt domain based on the 3D model included hormone binding [GO:0042562], isoprenoid binding [GO:0019840] and ...
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Le CpG et le poly(I:C) agissent en synergie avec le trastuzumab contre le cancer du sein HER2+
... both CpG ODN and poly(I:C) are able to enhance the functions of NK cells, but that simultaneous stimulation of the TLR-3 and TLR-9 allows to maximize the beneficial effects of ...both CpG ODN and poly(I:C) ...
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Structural basis for the inhibition of HIV-1 Nef by a high-affinity binding single-domain antibody
... Although compounds interfering with Nef's activity would be in multiple ways beneficial to the host, Nef is currently not a target of antiviral measures. The Nef protein is not essential for replication of HIV in the in- ...
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Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences
... probe could function to measure these cytokine levels in cells under different stimuli and therefore serve as a read-out of inammation. Similarly, TTP recognizes and destabilizes a large number of tumor-related mRNAs ...
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Electrotransfer of CpG free plasmids enhances gene expression in skin
... as CpG motifs [6, ...200 CpG moieties [9, ...and CpG-free plasmids [11]. A CpG adjuvant effect was required for DNA vaccines, but was not needed and perhaps harmful for a local gene ...these ...
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Insights into the function of the CRM1 cofactor RanBP3 from the structure of its Ran-binding domain.
... shows the corresponding experiment for full-length RanBP3), in agreement with a previous semi-quantitative study [18]. We therefore pursued the structure of the RBD in its unbound form. We solved the structure at 1.61 A ...
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