Laser Capture Microdissection (LCM)

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Transcriptomic analysis of trout gill ionocytes in fresh water and sea water using laser capture microdissection combined with microarray analysis

Transcriptomic analysis of trout gill ionocytes in fresh water and sea water using laser capture microdissection combined with microarray analysis

Fish conditioning and sampling Immature rainbow trout (Oncorhynchus mykiss) bred in a local hatchery (Drennec, Sizun, France) were transferred to the laboratory facilities. After acclimation to the tanks for at least 1 week, the fish were directly transferred from fresh water to either fresh water (control group) or 35 ppt sea water. The protocol to transfer fish to SW was previously described [ 24 ]. The trout were maintained in a recirculated water system at 10 –13°C under a natural photoperiod and fed commercially available pellets (BioMar, Nessac, France). Fish (90–140 g), kept either in fresh water or transferred into sea water, were collected at various time points following the salinity change. After euthanized with a lethal dose of phenoxyethanol, blood and gill tissue were sampled. Plasma was stored at -20°C until further use. The second gill arches were col- lected for laser capture microdissection (LCM) and in situ hybridization (ISH). For LCM, the gill arches were frozen in isopentane by submersion in liquid nitrogen and storage at -80°C until further use. For ISH, the gill arches were fixed for 8 hours at 4°C with 4% paraformalde- hyde in phosphate buffered saline, then gradually dehydrated in methanol and stored in 100% methanol at −20°C.
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Identification of Vascular Breast Tumor Markers by Laser Capture Microdissection and Label-Free LC-MS.

Identification of Vascular Breast Tumor Markers by Laser Capture Microdissection and Label-Free LC-MS.

For the publisher’s version, please access the DOI link below./ Pour consulter la version de l’éditeur, utilisez le lien DOI ci-dessous. https://doi.org/10.1021/pr101267k Access and use of this website and the material on it are subject to the Terms and Conditions set forth at Identification of Vascular Breast Tumor Markers by Laser Capture Microdissection and Label-Free LC-MS.

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Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation

Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation

Laser capture microdissection and RNA extraction PET-coated slides were placed face up, dried directly after staining, and a 0.2-ml Eppendorf tube cap was placed on the dedicated tubing rack of the UV laser mi- crodissection PALM Microbeam (Carl Zeiss, Germany). Samples were processed within 2 h with LCM settings as follows: cut energy, 41; cut focus, 82; LPC energy delta, LPC focus delta 2; and 20 μm spot distances. After stamping CD3-immunolabeled cells at ×40 objective under Zeiss 43HE filter set to localize T cell infiltrated zones in the EAE white matter, contours of GFAP- labeled astrocytes were carefully drawn at ×40 objective using Zeiss 38HE filter set (Fig. 1a, b). White matter GFAP-labeled astrocytes from control mice were directly selected under the 38HE filter set. At the end of the drawings, astrocytes were microdissected and catapulted in the 0.2-ml Eppendorf tube cap filled with RLT lysis buffer (RNeasy Micro kit, Qiagen, Hilden, Germany) containing 1 % beta-mercaptoethanol. For each mouse, ~5.10 6 μm 2 of pooled astrocytes were then extracted for total RNA and Dnase I treated with RNeasy Micro kit. RNA quantity and integrity were evaluated using the 2100-Bioanalyzer with the RNA 6000 Pico kit (Agilent Technologies). Astrocyte samples (corresponding to 4000–6000 cells) gave 5–7 ng RNA. To analyze more than a few genes, a preamplification step was thus
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Amélioration de la capture des visages pour l'industrie du jeu vidéo

Amélioration de la capture des visages pour l'industrie du jeu vidéo

Nous allons ici nous intéresser à deux états de l’art en suivi de points 2D et 3D sur un visage. Le défi «300 Videos-in-the-Wild», dont les résultats sont présentés dans [Shen et al. (2015)], repose entièrement sur ce problème. Le but du défi est de trouver le meilleur algorithme permettant de suivre 68 points sur des visages extraits de vidéos d’une minute environ. Les vidéos sont triés selon trois scénarii de difficultés croissantes : le premier présente des vidéos dans un environnement à éclairage contrôlé, sans grands mouvements de tête ni occlu- sions. C’est le cas le plus proche d’une capture en laboratoire. Le second scénario présente des vidéos dans des situations d’éclairages réelles et avec des mouvements de tête non contrôlés. Ce scénario évite toutefois les occlusions. Le dernier scénario présente le reste des vidéos, les plus difficiles pour le suivi de points, avec un éclairage non contrôlé et présence d’occlusions. Les méthodes présentant les meilleurs résultats pour les 3 problèmes sont [Xiao et al. (2015)] et [Yang et al. (2015)].
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Estimation de la taille de la population dans les expériences de capture-recapture

Estimation de la taille de la population dans les expériences de capture-recapture

Ce travail considère une expérience de capture-recapture imbriquée avec deux niveaux d'échantillonnage : à l'intérieur de chaque période primaire d'un modèle de population ouverte, il y'a des occasions de capture secondaires pour estimer la taille de la population à la période primaire correspondante. Cette expérience est connue sous le nom de design robuste. Deux sources d'informations sont disponibles pour estimer la taille de la popu- lation à une période primaire : les données inter et intra primaire. Ce travail démontre que les estimateurs de la taille de la population obtenus à partir de ces deux sources d'informations sont asymptotiquement indépendantes pour une large classe de modèles de population fermée. Dans ce contexte, il est démontré que l'estimateur du maximum de vraisemblance pour la taille de la population obtenu sous le design robuste est asymptoti- quement équivalent à une somme pondérée des estimateurs pour le modèle de population ouverte de Jolly-Seber ( Jolly 1965 ; Seber 1965 ) et pour le modèle de population fermée. Cet article montre que cet estimateur pondéré est plus ecace que l'estimateur des mo- ments de Kendall et al. ( 1995 ). Une formule explicite de l'ecacité associée à l'estimateur de Kendall est donnée et la perte de précision évaluée via une étude de simulation et à travers un exemple sur l'estimation de la taille d'une population de dauphins présenté dans Santostasi et al. ( 2016 ).
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Capture orbitale martienne à l'aide de la traînée aérodynamique

Capture orbitale martienne à l'aide de la traînée aérodynamique

Puisque la transition s'effectue généralement dans les environs du point de plus basse al­ titude, le hs(h) équivalent peut être calculé jusqu'au mom ent de la transition. À partir de ce point, où le véhicule devrait être dans la phase de sortie, la valeur hs(h) est interpolée à partir des valeurs tabulées pendant la phase de capture. Cette valeur de hs(h) est consi­ dérée par l'algorithm e de guidage dans le calcul de la perte de vitesse aérodynamique. Cette m éthode présente l'avantage de représenter, par un modèle simple, une atm osphère avec une altitude d'échelle qui peu t varier de façon quelconque. Par exemple, si hs varie linéairement en fonction de l'altitude (figure 7.2), la m éthode fournit u n hs qui donne la même perte de vitesse aérodynam ique que l'atm osphère réelle pour une vitesse d'ascen­ sion constante et en négligeant les effets de la gravité (figure 7.3). En début de trajectoire (haute altitude), cette valeur tend vers l'altitude d'échelle locale.
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Revisiting the human uncinate fasciculus, its subcomponents and asymmetries with stem-based tractography and microdissection validation

Revisiting the human uncinate fasciculus, its subcomponents and asymmetries with stem-based tractography and microdissection validation

These results first confirm why detailed fiber dissection is possible using Klingler’s method, but also explain why studying fiber crossings during dissection is problematic, as the anatomist has to cut the crossing fibers to continue following a bundle of interest. This might be rather considered as an advantage since Klingler dissection can efficiently track one specific bundle through a crossing region. We recently demonstrated that the callosal fibers can be dissected in the frontal cortex up to their cortical termination by performing a layer-by- layer dissection through the crisscross of the association pathways (Sarubbo et al., 2016). In the present study, once the UF fibers exposed around the anterior and ventral third of the external and extreme capsules, we were able to dissect the complete UF fibers with a progressive removal of the white matter layer-by-layer through the crisscross of the projections fibers passing through the anterior arm of the internal capsule, the callosal fibers and some adjacent association patways (SLF, AF, IFOF). Despite the unique chance to follow a specific bundle starting from the dense core (i.e. the stem) up to the cortical terminations (according to the cortex sparing technique applied in this study), the blunt microdissection did not provide quantitative data regarding these terminations nor additional information about further cortical territories of terminations (i.e. false-negatives) or the full pattern of reciprocal connectivity encompassed by UF fibers. But combining in vivo anatomically constrained diffusion tractography with post-mortem Klingler dissection appreciably reduces these limitations to more reliably describe the white matter pathways.
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A Global Framework for Motion Capture

A Global Framework for Motion Capture

Unité de recherche INRIA Rennes IRISA, Campus universitaire de Beaulieu - 35042 Rennes Cedex France Unité de recherche INRIA Lorraine : LORIA, Technopôle de Nancy-Brabois - Campus scient[r]

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CO2 CAPTURE FOR MINERALIZED MISCANTHUS AGGREGATES

CO2 CAPTURE FOR MINERALIZED MISCANTHUS AGGREGATES

Figure 3: Evolution of CO 2 capture vs time Moreover, we note that miscanthus carbonated aggregates are more resistant to wear than those who were not carbonated (Table 5). A Micro-Deval test was performed on the miscanthus chips. This test was adapted to vegetal fibers following the procedure adopted by Grimont to avoid the total destruction of the fibers [12]. 250 grams of miscanthus aggregates were introduced in the cylinder with 50 stainless steel balls. It was then rotated at 100 rpm for 10 minutes. Results showed that carbonated chips have a Micro-Deval coefficient of about 7.23, while those that were not carbonated get a coefficient approaching 12.69, which corresponds to a higher loss of mass during attrition process. We can say that the CO 2
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Capture à grande échelle de trafic eDonkey

Capture à grande échelle de trafic eDonkey

5 Anonymisation et formatage L’anonymisation de traces internet est en soi un sujet d´elicat [AP07]. Nous avons choisi ici une approche extrˆemement prudente puisque nous anonymisons les clientID, les fileID, les chaˆınes de caract`eres utilis´ees dans les recherches et les noms de fichiers, et les tailles de fichiers. De plus, l’horodatage des donn´ees commence `a 0 afin de ne pas fournir la date exacte de capture. Chacune de ces donn´ees `a anonymiser a ses sp´ecificit´es dont nous avons dˆu tenir compte.

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Capture and control of excitations

Capture and control of excitations

𝑅 = {1, 𝜃 ≥ 𝜃 𝐶𝑟𝑖𝑡𝑖𝑐𝑎𝑙 0, 𝜃 < 𝜃 𝐶𝑟𝑖𝑡𝑖𝑐𝑎𝑙 Above the critical angle, total internal reflection occurs, and the photon can survive any number of reflection events. In the absence of re-absorption as in our example, these photons will reach the far end of the device. Below the critical angle, the number of reflection events required for capture is simply too many to have an appreciable chance of survival. By allowing photons to escape perhaps a few reflection events earlier than the correct physical model would suggest, our Monte Carlo simulation culls non-viable photons, thus speeding up the simulation. We might have discovered this by varying the implementation of reflection physics and noticing what variations do not change the results over a variety of conditions, and taught ourselves a lesson about what physical processes within such a device matter for efficient photon collection.
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Algorithme générique pour les jeux de capture dans les arbres

Algorithme générique pour les jeux de capture dans les arbres

ST v F IG . 1: Exemple d’arbres dont les vecteurs associ´es sont vect (w) = (1, 2) et vect(w) = (3, 3). vertex separation, le node search number et la pathwidth sont ´equivalents. Le lecteur est invit´e `a lire l’´etat- de-l’art ´etablit par Fomin et Thilikos [4] pr´esentant de nombreuses variantes des probl`emes de capture.

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Le Ru de Pôleur ancien et sa capture par le Hoëgne

Le Ru de Pôleur ancien et sa capture par le Hoëgne

Pour discuter les conditions de cette capture, il faut garder à l’esprit quelques étapes de la formation des Hautes Fagnes (Boulvain et Juvigné, 2010) évoquées ci-après. - A la fin du Crétacé (65 Ma; Ma = million d'années), la mer a noyé le NO de l’Ardenne jusqu’à la crête des Hautes Fagnes et au-delà, parachevant l’aplanissement du relief ardennais qui avait commencé dès la fin de l'orogenèse hercynienne (~300 Ma) ; elle y a ensuite

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Optimal Carbon  Capture and Storage policies

Optimal Carbon Capture and Storage policies

3- The site of In Salah, which like Sleipner and Snøhvit is a natural gas reservoir located in In Salah, Algeria. The CO2 will be separated from the natural gas and re-injected into the subsurface at a rate of about 1.2 million tonnes per year. 4- In July 2008, the Government of Alberta announced a $2 billion investment in three to ve large-scale carbon capture and sequestration projects. On June 30, 2009, Government announced three projects it will pursue letters of intent with and work to have the letters signed in the fall. If discussions with these proponents are not successful, Government will evaluate its options and may proceed to discussions with other proponents.
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Capture-induced transition in foamy suspensions

Capture-induced transition in foamy suspensions

Capture-induced transition in foamy suspensions Y. Khidas, a B. Haffner b and O. Pitois* b We investigate the drainage behaviour of foamy granular suspensions. Results reveal large fluctuations in the drainage velocity as bubble size, particle size and gas volume fraction are varied for a given particle volume fraction. Particle capture is proved to control the overall drainage behaviour through the parameter l, which compares the particle size to the size of passage through constrictions within the foam pore space. l highlights a sharp transition: for l < 1 particles are free to drain with the liquid, which involves the shear of the suspension in foam interstices, for l > 1 particles are trapped and the resulting drainage velocity is strongly reduced. A phenomenological model is proposed to describe this behaviour.
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Capture and decay of electroweak WIMPonium

Capture and decay of electroweak WIMPonium

Our cross section result in the unbroken SU(2) limit includes a factor of 1/3 from the overlap between our initial condition (particles begin as neutralinos) and the eigenvector of the potential matrix that experiences an attractive interaction; in the language of appendix C , and particularly eq. C.18 , this factor appears in the matrix element as I · η i (= 1/ √ 3 for the wino). More specifically, it appears in the contribution to the matrix element from each component of the continuum wavefunction that experiences an attractive interaction. In the low-velocity limit, it is these contributions that control the overall capture rate, since any component of the wavefunction that experiences a repulsive interaction is suppressed toward the origin and its contribution to the capture rate is exponentially suppressed (as we demonstrate in appendix C ). In the unbroken SU(2) limit, the wavefunctions associated with the various eigenvectors of the potential obey decoupled Schrödinger equations, and the eigenvectors themselves are independent of r; thus we can determine the wavefunctions associated with the various eigenvectors at some large r (i.e. by setting an initial condition), and then evolve them straightforwardly for all r.
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Automated Design Data and Rationale Capture

Automated Design Data and Rationale Capture

6.1 Knowledge Acquisition The graphical user interface has been designed to assist in knowledge acquisition during intelligent capture of simulation exercises. For example, the history table feature visually conveys the degree of stability of parameters in the system. Users can easily identify the volatilities of different parameters across iterative design sessions. Also, by storing Error objects in the data model, the user can analyze potential system problems that are currently very difficult to analyze or even recognize.

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Etude et simulation de la réponse d’ une capture microbalance à quartz

Etude et simulation de la réponse d’ une capture microbalance à quartz

Dans le cas d’un quartz recouvert d’une couche sensible élaborée à base de vapeur de TEOS pure, l’étude théorique montre que la couche d’épaisseur 690 nm possède la plus grande valeur [r]

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Accelerator-based boron neutron capture therapy

Accelerator-based boron neutron capture therapy

For the purpose of this thesis, the term Accelerator-Based BNCT (AB-BNCT) will be used to describe the production of neutrons through charged particle induced react[r]

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Efficient IP-level network topology capture

Efficient IP-level network topology capture

2 A Generic Distributed Tracing (GDT) framework Distributed network tracing systems tend to be similar to each other. Each has a number of lightweight agents and a heavier weight central server. Probing is conducted in rounds, with each agent working from a fixed set of instructions for a round. Results from the agents are sent back from time to time to the central server. We formalize these notions into a Generic Distributed Tracing (GDT) framework. The framework leaves room for many different specific prob- ing heuristics to be applied. The following section describes related work in the context of this framework, and the section after that describes our own NTC (network topology capture) heuristics.
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