Kinase activity
Mnk1 kinase activity is required for abscission.
... or kinase-dead MNK1 (Pyronnet and Sonenberg, ...the kinase-dead MNK1 increased this number up to 16% (61) ...the kinase-dead MNK1 indicate that the kinase activity of MNK1 is required ...
10
A novel ZAP-70 dependent FRET based biosensor reveals kinase activity at both the immunological synapse and the antisynapse
... Reveals Kinase Activity at both the Immunological Synapse and the ...enzyme activity or dynamic posttranslational modifications such as phosphorylation, from the translocation or redistribution of a ...
7
LRRK2 impairs PINK1/Parkin-dependent mitophagy via its kinase activity: pathologic insights into Parkinson’s disease
... PARK2 mutations and carriers of the G2019S LRRK2 mutation (Fig. 5A and C). The levels of red-only fluorescence in cells from patients were similar to those in control cells maintained at 37°C (Fig. 3B), suggesting that ...
52
Fluorescent Biosensor of CDK5 Kinase Activity in Glioblastoma Cell Extracts and Living Cells
... overall kinase activity, in contrast with genetic, transcriptomic or proteomic approaches which inform on gene, transcript or protein expression ...few kinase activity reporters which enable ...
24
Interaction between the tRNA-Binding and C-Terminal Domains of Yeast Gcn2 Regulates Kinase Activity In Vivo
... Gcn2 activity by directly interacting with the KD, and proposed that the inhibitory CTD/KD interaction would compete with this stimulatory YKD/KD interaction, and that tRNA binding to the HisRS domain would shift ...
28
Cell surface adenylate kinase activity regulates the F(1)-ATPase/P2Y (13)-mediated HDL endocytosis pathway on human hepatocytes.
... higher activity observed on primary hepatocytes as compared to HepG 2 cells may be attributed to an increased metabolism already described in primary cultures [27, ...adenylate kinase as shown by the 68% ...
19
In Vitro and In Vivo Approaches to Assess Rho Kinase Activity
... vascular cells and tissues from patients or animal models, gives access to the kinase activity in different physiological or pathological situations. This assay consists in measuring, by scintillation ...
10
Assessment of DNA-PKcs kinase activity by quantum dot–based microarray
... Florian Lafont 1 , Nizar Ayadi 1 , Cathy Charlier 1 , Pierre Weigel 1 , Igor Nabiev 2,3 , Houda Benhelli-Mokrani 1 & Fabrice Fleury 1 Therapeutic efficacy against cancer is often based on a variety of DNA lesions, ...
13
AKT1 restricts the invasive capacity of head and neck carcinoma cells harboring a constitutively active PI3 kinase activity
... AKT1 activity promotes early stages of tumorigenesis but restricts the tumor cell meta- static ...specific activity is also involved in the mainten- ance of the epithelial phenotype of HNSCC ...AKT/mTOR ...
11
Aurora A kinase activity is required to maintain an active spindle assembly checkpoint during prometaphase
... A activity is required to maintain Mad2 at non-attached kinetochores during prometaphase, ensuring that the SAC remains ...the kinase inhibition (MLN8237) leads to its retention at ...A kinase ...
39
AMP-Activated Protein Kinase Signalling
... Starting from the AMPK molecule, Yan et al. summarize the knowledge derived from crystal structures and provide expert insight into the molecular mechanisms of kinase activity modulation by adenine ...
6
Rapid Diminution in the Level and Activity of DNA-Dependent Protein Kinase in Cancer Cells by a Reactive Nitro-Benzoxadiazole Compound
... DNA-PKcs activity was assessed by the phosphorylation rate of its substrate, RPA2, when cells were induced by a selective topoisomerase I poison, CPT [ 26 ...cyclin-dependent kinase (CDK) and by PIKKs ...
17
Étude de l'extension N-terminale de la kinase mitotique MPS1
... its kinase activity (A) Hela S3 cells were transiently transfected with MYC-FKBP-MPS1 WT, KD, ΔNTE and MYC-MPS1ΔNTE together with siRNA MPS1 for endogenous MPS1 ...
202
The catalytic activity of the kinase ZAP-70 mediates basal signaling and negative feedback of the T cell receptor pathway
... the kinase activity of ZAP-70 Our understanding of the mechanisms responsible for feedback and regulation within the TCR signaling network is far from ...its activity might also be needed to properly ...
31
Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology
... enhanced nucleoside phosphorylation produced by masitinib as the wild-type (Supplementary Fig. 15 ). Analysis of the effect of masitinib on UCK1 activity was performed using ATP as phosphate donor. PK/LDH coupled ...
12
A generic HTS assay for kinase screening: Validation for the isolation of an engineered malate kinase
... enzyme activity, was ...pyruvate kinase and lactate ...malate kinase activity in a structurally-guided library of LysC aspartate kinase variants comprising 6,700 ...novel kinase ...
16
Dual regulation of activity and intracellular localization of the PASTA kinase PrkC during Bacillus subtilis growth
... We then decided to use the disaccharide tetrapeptide GlcNAc-MurNAc-L-Ala-γ-D-Glu-meso-DAP-D-Ala (or TCT) 34 in our tests (Fig. 3A ). As purified muropeptides are usually used at the micromolar range in the previously ...
13
Aurora B kinase, an immobile passenger!
... Aurora kinase and its partners? Aurora kinase phosphorylates close substrates and the cascade of phosphorylation is regulated by the tension across kinetochores ( ...Aurora kinase activity ...
3
Les polyoxométallates - Des inhibiteurs inorganiques de la protéine kinase CK2
... [1-3] . L’expression de CK2 est déré- gulée dans un large panel de cancers et son activité kinase est corrélée à la progression tumorale. Ainsi, des tra- vaux de plusieurs laboratoires, dont le nôtre, ont montré ...
3
Préparation de prodrogues d'inhibiteurs de kinase : synthèse de phosphoramidates
... Elle-même est contrôlée par différents facteurs dont les polycystines ; ainsi, quand l’activité ciliaire est normale, la protéine kinase est inhibée (ou n’a en tout cas pas d’activité décelable). A l’inverse, il a ...
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