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Inducible expression

BBF RFC #111: Inducible expression vector with His tag fusion for purification of RFC25 parts

BBF RFC #111: Inducible expression vector with His tag fusion for purification of RFC25 parts

... new expression vector based on the pET expression system and designed for BioBrick RFC25 ...pET expression vectors with a new origin of replication, resistance marker and Multiple Cloning Site ...new ...

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Salt-inducible expression of OsJAZ8 improves resilience against salt-stress

Salt-inducible expression of OsJAZ8 improves resilience against salt-stress

... own expression, constructs driving full and C-terminal trun- cated version of OsJAZ8 genes under the control of salt-induced promoter ZOS3–11 (Additional file 1 ) were used to suppress jasmonate signalling ...

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Process development for an inducible rituximab-expressing Chinese hamster ovary cell line

Process development for an inducible rituximab-expressing Chinese hamster ovary cell line

... mammalian inducible cell systems are becoming increasingly available, very little information is available in the open literature regarding process development for these types of ...cumate-inducible ...

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Comprehensive Characterization of an Inducible Mammalian Expression System Using 13C-Metabolic Flux Analysis

Comprehensive Characterization of an Inducible Mammalian Expression System Using 13C-Metabolic Flux Analysis

... protein expression, the percentage of the pyruvate pool entering the TCA was relatively high in both cultures (induced and non-induced cells) as compared to most published flux distributions of cultivated animal ...

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5 years of successful inducible transgene expression following locoregional AAV delivery in nonhuman primates with no detectable immunity

5 years of successful inducible transgene expression following locoregional AAV delivery in nonhuman primates with no detectable immunity

... transgene expression in large animal models of rAAV-mediated gene ...persistent inducible transgene expression for at least 1 year under the control of the tetracycline-inducible TetON system, ...

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A self-inducible heterologous protein expression system in Escherichia coli

A self-inducible heterologous protein expression system in Escherichia coli

... The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in ...(Self-InducibLe Expression) system, which is a convenient, cost-effective ...

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Gene silencing using a heat-inducible RNAi system in Arabidopsis.

Gene silencing using a heat-inducible RNAi system in Arabidopsis.

... gene expression by sense, antisense, and RNA interference (RNAi) can be considered as powerful complementary tool (Bourque, 1995; Chuang and Meyerowitz, ...gene expression in transgenic plants is observed ...

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A human beta cell line with drug inducible excision of immortalizing transgenes.

A human beta cell line with drug inducible excision of immortalizing transgenes.

... proliferation and pronounced enhancement of beta-cell specific fea- tures such as insulin expression, storage in secretory granules and glucose stimulated secretion. These properties were maintained in culture for ...

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Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.

Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells.

... 22. Geller D. A., Lowenstein C. J., Shapiro R. A., Nussler A. K., Di Silvio M., Wang S. C., Nakayama D. K., Simmons R. L., Snyder S. H., and Billiar T. R. (1993) Molecular cloning and expression of ...

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Construction of a set of vectors allowing inducible production of siRNA in Schizosaccharomyces pombe.

Construction of a set of vectors allowing inducible production of siRNA in Schizosaccharomyces pombe.

... In contrast to budding yeast, the entire pathway is conserved in the fission yeast Schizosaccharomyces pombe, making it a valuable organism to both study physiological RNAi and to use it as a inducible gene ...

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Hypoxia-inducible factor (HIF1α) gene expression in human shock states.

Hypoxia-inducible factor (HIF1α) gene expression in human shock states.

... expression may be more accurate in tissue than in blood. However, regarding our study goals, the collec- tion of tissue biopsy was irrelevant. Second, we may hypothesize that the protein of HIF1a may better ...

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Differential Effects of STCH and Stress-Inducible Hsp70 on the Stability and Maturation of NKCC2

Differential Effects of STCH and Stress-Inducible Hsp70 on the Stability and Maturation of NKCC2

... sum, these findings are in accordance with the results of Y2H and co-immunoprecipitation studies and strongly suggest that NKCC2 and STCH interact mainly at the ER. 2.2. Site of NKCC2 Interaction with STCH Previous ...

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Plant growth promoting and inducible antifungal activities of irrigation well water-bacteria

Plant growth promoting and inducible antifungal activities of irrigation well water-bacteria

... For many years, extracellular enzymes secretion has been associated with biocontrol abilities of the producing bacteria. Thus, some bacterial enzymes are able to destroy fungal spores, a ffecting their germinability and ...

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Arg375 tunes tetrahydrobiopterin functions and modulates catalysis by inducible nitric oxide synthase.

Arg375 tunes tetrahydrobiopterin functions and modulates catalysis by inducible nitric oxide synthase.

... thesis of the enzyme, we generated and characterized three mutants R375D, R375K and R375N of the oxyge- nase domain of inducible NOS (iNOSoxy). The mutations affected the dimer stability of iNOSoxy and its binding ...

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Inducible nitric oxide synthase mediates retinal apoptosis in ischemic proliferative retinopathy.

Inducible nitric oxide synthase mediates retinal apoptosis in ischemic proliferative retinopathy.

... role of iNOS on retinal apoptosis in ischemic proliferative reti- nopathy. Using iNOS knock-out mice and iNOS inhibitor 1400W, we demonstrate in vivo that iNOS expression induces apoptosis locally in the inner ...

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The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry

... inactivated (HI) virus control was used in parallel. Bound viruses were removed by trypsinization of the cells. The cells were fixed, permeabilized and stained with an anti-NP antibody[r] ...

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Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis

Inducible Treg cell populations as cell based-therapy for rheumatoid arthritis

... Access Inducible Treg cell populations as cell based- therapy for rheumatoid arthritis Delphine Martire 1 , Julie Quentin 1 , Anne-Laure Mausset-Bonnefont 1 , Hélène Asnagli 2 , Nathalie Belmonte 2 , Arnaud ...

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Rôle du hypoxia inducible factor 1α (HIF-1α) durant la leishmaniose viscérale

Rôle du hypoxia inducible factor 1α (HIF-1α) durant la leishmaniose viscérale

... the expression of CD41, CD150, CD16/32 to define granulocyte- monocyte progenitors (GMP) as CD16/32 hi CD150 - cells ...Sca-1 expression on HSPCs ...

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Interferon-inducible protein (IFI) 16 regulates Chikungunya and Zika virus infection in human skin fibroblasts.

Interferon-inducible protein (IFI) 16 regulates Chikungunya and Zika virus infection in human skin fibroblasts.

... Expression of IFI16 restricts Chikungunya virus infection Our previous study demonstrated the im- mune sensor, AIM2, reduces CHIKV replica- tion by the induction of the inflammasome in HFF1 cells (Ekchariyawat et ...

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Communication, fiction et expression de la subjectivité

Communication, fiction et expression de la subjectivité

... Or, si les énoncés au style indirect libre comprennent parfois une postface locutionnaire (ou indiquant une attitude propositionnelle quelconque), dont on peut considérer qu’elle a dan[r] ...

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