human induced pluripotent stem cells
Transgene-free hematopoietic stem and progenitor cells from human induced pluripotent stem cells
... H. Lapillonne et al., Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine. Haematologica. 95:1651-1659 (2010 ). Aknowledgments: We ...
46
Functional Impact of BeKm-1, a High-Affinity hERG Blocker, on Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells
... pharmacological compounds is associated to drug-induced long QT syndrome (LQTS), which is a life-threatening disorder characterized by ventricular arrhythmias and defects in cardiac repolarization that can be ...
15
Modeling human early otic sensory cell development with induced pluripotent stem cells
... from human induced pluripotent stem cells ...hair cells, may provide an unlimited supply of cells for research and cell-based ...progenitor cells were then treated ...
21
Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia
... urine-sample-derived human induced pluripotent stem cells (UhiPSCs) as an appropriate tool to model PCSK9-mediated ADH and ...somatic cells were reprogrammed into hiPSCs by using ...
11
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
... apoptotic cells 13 ...of cells undergoing apoptosis (see Supplementary ...cryopreserved cells were of high quality and the nucleosome laddering was detected by gel electrophoresis ...fresh ...
11
Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells
... differentiated cells derived from induced pluripotent stem cells (iPSCs), primary cell culture, and lim- ited clinical ...of cells or even single cells with- out requiring ...
11
Generation of induced pluripotent stem cells-derived hepatocyte-like cells for ex vivo gene therapy of primary hyperoxaluria type 1
... the human AGT yielded encouraging results in AGXT −/− ...wild-type human AGXT gene into hepatic cells using lentiviral vectors ...primary human hepatocytes, but the invasiveness of liver ...
12
Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells
... Trithorax group (TrxG) and Polycomb group (PcG) protein complexes are key regulators of chromatin structures that are required for segmental identity in the developing embryo and contribute to maintenance of the ...
10
Evaluating the potential of poly(beta-amino ester) nanoparticles for reprogramming human fibroblasts to become induced pluripotent stem cells
... over a 4-day period, the embryoid bodies were transitioned from mTeSR1 to neural induction medium comprised of DMEM:F12 (1:1) containing 2 mM glutamine, 1% N2 supplement, 1 × nones- sential amino acids, and heparin ...
19
Pluripotent stem cell-derived cochlear cells: a challenge in constant progress
... hair cells (around 1500 per aggregate) but also cells expressing supporting cell markers [ 1 , 34 – 36 ...]. Human models Mouse models are powerful research tools to study develop- mental mechanisms ...
9
NOTO Transcription Factor Directs Human Induced Pluripotent Stem Cell-Derived Mesendoderm Progenitors to a Notochordal Fate
... founder cells of the Nucleus pulposus, the centre of the intervertebral disc, originate in the embryonic ...notochordal cells (NC) are identified as key regulators of disc ...Using human ...
25
Extended passaging increases the efficiency of neural differentiation from induced pluripotent stem cells.
... that human iPSC lines derived using disparate methods ...as cells derived using retroviruses, which suggests that variability is independent of deriva- tion ...late-passage human iPSCs performed ...
15
The Potential of Induced Pluripotent Stem Cells to Test Gene Therapy Approaches for Neuromuscular and Motor Neuron Disorders
... somatic cells into induced pluripotent stem cells (iPSCs) represents a major advance for the development of human disease ...
13
Human Induced Pluripotent Stem Cell-Derived Astrocytes Are Differentially Activated by Multiple Sclerosis-Associated Cytokines
... precursor cells and minimize the concomitant formation of neurons, most authors add FBS to the differentiation medium ( Tyzack et ...we induced the neuralization of hiPSCs into neural stem ...
13
Derivation of induced pluripotent stem cells from an infertile boar carrying a reciprocal translocation
... germ cells from embryonic (ES) or induced (iPS) pluripotent stem cells in mouse and human is a great advance for the study of germ cell biology in the earlier step of development ...
2
Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells
... Both human embryonic stem (hES) cells and induced pluripotent stem (hiPS) cells can self-renew indefinitely in culture, however current methods to clonally grow them are ...
23
Embryo-derived and induced pluripotent stem cells: Towards naive pluripotency and chimeric competency in rabbits
... 3. Pluripotent state of rbiPSCs Rabbit induced PSCs (rbiPSCs) have been obtained after repro- gramming somatic cells from the stomach, liver and skin using retro- viral vectors expressing ...
9
Modeling susceptibility to drug-induced long QT with a panel of subject-specific induced pluripotent stem cells
... The risk of developing diLQT varies markedly between subjects. Previous studies in the general population have shown that a small proportion (i.e., less than 10%) of patients exposed to QT-pro- longing drugs actually ...
25
en fr Interest of rabbit induced pluripotent stem cells for species preservation – Intérêt des cellules souches pluripotentes induites de lapin pour la conservation de cette espèce
... C., R OGNARD C., H OCHARD A., V OISIN S., F ONTAINE E., M URE M., A FANASSIEFF M., C LEROUX E., G UIBERT S., C HEN J., V ALLOT C., A CLOQUE H., G ENTHON C., P AIN B., B OURILLOT P.Y., AND S AVATIER P. 2015. Reinforcement ...
13
Embryonic stem cells or induced pluripotent stem cells? A DNA integrity perspective.: DNA integrity of iPS cells
... undisputedly demonstrating that complete reprogramming had occurred. Unexpectedly, the authors found only one or two de novo structural genomic variations in each iPSC line. As a matter of fact, the most important source ...
8