Human cells
An Adaptable Platform for Directed Evolution in Human Cells
... the human genome by using somatic hypermutation 1112 or Cas9-fusion proteins 13-15 still display significant off-target genetic ...the human genome, many pathways to cheating selection may be ...the ...
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GC content shapes mRNA storage and decay in human cells
... an intrinsic property of these mRNAs and not simply the result of their sequestration in PBs ( Hubstenberger et al., 2017 ). Interestingly, the mRNAs of haplo-insufficiency genes, which by defi- nition are expected to ...
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GC content shapes mRNA decay and storage in human cells
... in human cells where most regulation at the level of translation is dedicated to AU-rich mRNAs, which have a limiting protein yield, whereas regulation at the level of 5’ decay applies to GC-rich mRNAs, ...
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Tropism of Tanapox virus infection in primary human cells.
... primary human dermal fibroblasts (pHDFs) and peripheral blood mononuclear cells (PBMCs), both of which are believed to be major in vivo targets of poxvirus ...TPV-GFP-infected human PBMCs were ...
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Genetic analysis of cell size homeostasis in human cells
... trillion cells, humans have cells with remarkably varied functions and size, ranging from a neuron that can reach one meter in length to a lymphoid cell that is around 16 µm in ...in human ...
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Bacterial FtsZ induces mitochondrial fission in human cells
... 672 C 673 Semiautomated quantification (MiNA plugin) of mitochondrial morphology expressed 674 by the amount of individual mitochondria/mitochondrial area in U2OS cells transfected [r] ...
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Proteome alteration induced by hTERT transfection of human fibroblast cells
... eukaryotic cells into replicative ...eukaryotic cells by adding telom- eric repeats to the 3' end of ...most human cells during embryogenesis, thereby limiting their proliferative capac- ...
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3D Chromosome Regulatory Landscape of Human Pluripotent Cells
... different cells and that transcriptional regulatory elements function within this context to produce cell-type specific gene expression ...37 human cell/tissue types (Figure ...of human cells, ...
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In vitro modeling of the neurovascular environment by coculturing adult human brain endothelial cells with human neural stem cells.
... rodent cells [23], assays composed of only human cells investigating the formation of vasculature-like structures (VLS) of brain microvascular endothelial cells by interacting with brain ...
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Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells
... xylasin. Human myoblasts were labelled with intravital fluorescence marker, PKH67 dye ...of cells were labelled by PKH67 (data not ...derived cells were iso- lated and plated for 24 hrs to recover ...
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Multicellular human alveolar model composed of epithelial cells and primary immune cells for hazard assessment
... untested human blood samples involves specific care to prevent the potential transmission of infectious diseases, such as HIV (human immunodeficiency virus), hepatitis B, and hepatitis ...
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αvβ3 integrin expression increases elasticity in human melanoma cells
... b [ 4 ]. Each subunit is composed of a large extracellular N-terminal domain that allows speci fic ligand binding, a single transmembrane-spanning helix and a short C-terminal cytoplasmic sequence that provides docking ...
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Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis
... during human lymphatic vessel formation. Here we show, using primary human dermal lymphatic endothelial cells (HDLECs), pharmacological tools (probenecid, Brilliant Blue FCF, mimetic peptides [ 10 ...
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Topography of cancer-associated immune cells in human solid tumors.
... Addressing the biological differences and the different responses to immunotherapy across tumor entities, we systematically compared different tumor types. We analyzed ten tumor entities in the framework of this ...
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Intramuscular transplantation in non-human primates of human muscle-derived stem cells (MuStem cells), a promising candidate for cell therapy of muscular dystrophies
... characterization. Human muscle- derived cells were isolated from skeletal muscles of 9 to 15-year- old patients free of known muscle ...disease. Cells were isolated based on a modified version of ...
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Human mucosal associated invariant T cells detect bacterially infected cells.
... MAIT Cells We next performed phenotypic analyses of Mtb-reactive MR1- restricted T-cell clones to determine if they shared properties of previously characterized MR1-restricted MAIT ...and human ...
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Role of XPC in the human cutaneous cancer cells invasion
... epidermal cells towards skin cancer could be elicited by the secretory phenotype of XP-C ...in cells morphogenesis, proliferation, malignant transformation and cancer progression (Kalluri and Zeisberg ...of ...
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MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
... images. Once the protocol has been validated by the user with his specific cells, mitochondria labeling will be left out for functional studies, to preclude possible biological biases. Dose-response analyses over ...
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Molecular signature of erythroblast enucleation in human embryonic stem cells
... ETHODS Cells and culture conditions The research on embryonic stem cells was authorized by the French Biomedicine Agency (R0500225A, R0500226A and ...
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Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells.
... L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignemen[r] ...
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