gene targeting
Gene targeting in maize by somatic ectopic recombination
... HR and non-homologous end-joining (NHEJ) are triggered to repair double-strand breaks (DSBs) of DNA. These lesions are formed accidentally by genotoxic stresses (Hanin and Paszkowski, 2003; Khanna et al., 2001; Tuteja et ...
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Deciphering the DSB repair mechanisms involved in CRISPR-induced mutagenesis and gene targeting in the model plant, Physcomitrella patens.
... Figure 4: Theoretical types of gene targeting (GT) events in P. patens using the CRISPR/Cas9 system, and procedure used to detect them and evaluate the frequency of gene targeting. Table 2: ...
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Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases
... In our study, we applied TALE nucleases to achieve the in- sertion of a large expression cassette or exon exchange through gene targeting by HDR in rat zygotes. For that purpose, we microinjected TALE ...
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CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens
... functional gene analysis, but also a promising approach for the development of new traits of interest in crops (Collonnier et ...to gene replacement through HDR-based repair, namely gene ...
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CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses
... CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses ABSTRACT A key goal for cell biological analyses is to assess the phenotypes that result from eliminating ...
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Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript
... of targeting ribozyme design software that have been done in the last decade: it finds target sites, computes target accessibility, ribozyme structure validity and potential off-target ...to gene ...
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Targeting the cytoskeleton against metastatic dissemination
... the targeting of the actin cytoskeleton through tropomyosin small molecule inhibitors sensitize cancer cells to anti-microtubule drugs, being also well tolerated in vivo [ 297 ...
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SMA: from gene discovery to gene therapy
... (Smn) gene, termed ...atrophy. Gene therapy, consisting of reintroducing SMN1 in the MNs, is an attractive approach for ...This gene the- rapy treatment obtained a marketing authorization by the FDA ...
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Targeting MUC4 in pancreatic cancer: miRNAs
... 3 its promoters [5]. This hypermethylation by DNMT3A/3B is linked with a repressive histone code including histone deacetylation by HDAC3. Post-transcriptional regulation by microRNAs (miRNA) is a new promising strategy ...
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Pharmacological Targeting of IRE1 in Cancer
... CONCLUDING REMARKS AND FUTURE PERPECTIVES The ER controls multiple cellular functions including protein folding, post- translational modifications (PTMs), fatty acid biosynthesis, detoxification, and intracellular ...
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Targeting a simple statistical bandit problem
... using the cv.glmnet function from package glmnet ( Friedman et al. , 2010 ), with weights given in ( 0.2 ) and the option "lambda.min". This means imposing (data-adaptive) upper-bounds on the ` 1 - and ` 2 -norms ...
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Bacteria-targeting nanoparticles for managing infections
... More specifically, steps were taken towards using these NPs (1) to achieve greater spatiotemporal control over drug therapy by more targeted antibiotic delivery to b[r] ...
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Targeting CD8 T-Cell Metabolism in Transplantation
... 15 ). Furthermore, memory CD8 T cells have been shown to act as major barriers for transplant tolerance induction protocols ( 16 , 17 ). Additionally, recent immunomonitoring research has shown that an increase in ...
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Neutralizing Antibodies Targeting HIV-1 gp41
... Trimeric MPER fusion proteins [ 150 ] Gp41 ectodomain fused to an influenza HA2 region [ 151 ] 10. Conclusions Much progress in understanding the molecular details of bnAbs targeting HR1, FP and MPER in ...
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Targeting Costimulatory Pathways in Systemic Sclerosis
... One small randomized, placebo-controlled trial assessed the efficacy of abatacept in patients with dcSSc over a period of 24 weeks ( 61 ). After randomization, 7 patients received abatacept therapy, while 3 patients in ...
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GOToolBox: functional analysis of gene datasets based on Gene Ontology.
... of gene function which are specific to few lineages ...responding gene product. The hierarchical organization of the gene ontology is particularly well adapted to computa- tional processing and is ...
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Targeting Cancer Stem Cells to Overcome Chemoresistance
... Active targeting of our hybrid gold nanoparticles on HER2-overexpressing breast cancer cells, in patient-derived xenografts of HER2-overexpressing breast cancer, increased gold delivery to cancer ...
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Biochemically informed modeling of miRNA targeting efficacy
... to gene-by-gene het- erogeneity in dosage sensitivities that resulted in a stronger signa- ture of up-regulation at more dosage-sensitive genes (Pessia et ...Z-linked gene expression in birds pro- ...
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Targeting IRAK1 in T-Cell acute lymphoblastic leukemia
... Knock-down of IRAK1 induces apoptosis and disrupts cell cycle in T-ALL To test whether IRAK1 is required for T-ALL survival, we transduced short hairpin RNAs (shRNA) targeting IRAK1 into T-ALL cell lines. Two ...
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Targeting CD137 enhances the efficacy of cetuximab.
... Despite the predominant role of NK cells and the innate immune response underlying the mechanism of synergy, we observed a role for CD8 + T cells in the antitumor response. In the combination of anti-CD20 mAb followed by ...
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