fluorescence correlation spectroscopy (FCS)
A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell
... CNRS, CIML, Parc scientifique de Luminy, Case 906, F-13288 Marseille, France (Dated: July 29, 2009) A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes ...
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Bayesian Total Internal Reflection Fluorescence Correlation Spectroscopy Reveals hIAPP-Induced Plasma Membrane Domain Organization in Live Cells
... vivo. Fluorescence correlation spectroscopy (FCS) is a powerful approach for probing the molecular dynamics of plasma membranes in live cells with a single-molecule sensitivity and the ability to ...
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Monitoring the interaction between DNA and a transcription factor (MEF2A) using fluorescence correlation spectroscopy.
... Fluorescence correlation spectroscopy (FCS) is an analytical method that allows distinguishing different populations of fluorescent probes in solution and provides data on their concentrations and ...
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Near-field optical probes provide subdiffraction-limited excitation areas for fluorescence correlation spectroscopy on membranes
... Ottawa, Ontario K1A 0R6, Canada; 2 Department of Physics and Astronomy, McMaster University, Hamilton, Ontario L8S 4M1, Canada Abstract : Near-field optical probes have been used to produce a subdiffraction-limited ob- ...
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Bayesian Model Selection Applied to the Analysis of Fluorescence Correlation Spectroscopy Data of Fluorescent Proteins in Vitro and in Vivo
... Abstract Fluorescence correlation spectroscopy (FCS) is a powerful technique to investigate molecular dynamics with single molecule sensitivity. In particular, in the life sciences it has found ...
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Bayesian Approach to the Analysis of Fluorescence Correlation Spectroscopy Data II: Application to Simulated and In Vitro Data
... ABSTRACT Fluorescence Correlation Spectroscopy (FCS) is a powerful approach to characterizing the binding and transport dynamics of ...image correlation spectroscopy, and therefore ...
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Multi-confocal fluorescence correlation spectroscopy in living cells using a complementary metal oxide semiconductor-single photon avalanche diode array
... simultaneous Fluorescence Correlation Spectroscopy measurements, by focusing the spots with a Spatial Light Modulator and acquiring data with a monolithic 32 × 32 single-photon avalanche photodiode ...
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Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy
... The fluorescence emitted from the sample is col- lected by the same objective, descanned, and focused again by a collecting lens into a confocal ...The fluorescence light after the confocal pin- hole is ...
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Multi-confocal fluorescence correlation spectroscopy.
... To overcome this barrier, it is necessary to develop multiple laser spot systems. Several techniques have already been used to produce separate laser spots. In one of the pioneering experiments, based on elastic ...
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Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.
... Concerning our study of heat shock, the mFCS experiments put into evidence weakly specific interactions of HSF1 with DNA sites homogeneously distributed within the nucleus, superimposed to more specific and much less ...
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en fr Molecular confinement and membrane organization of live cells: analysis of diffusion by fluorescence correlation spectroscopy Confinement moléculaire et organisation de la membrane des cellules vivantes: analyse de la diffusion par spectroscopie de corrélation de fluorescence
... les cellules COS-7. 3.1.1 Procédure de positionnement de la membrane dans le volume confocal Il est d’abord nécessaire de placer la membrane cellulaire dans le plan de sy- métrie (xy) du volume confocal. La cellule ...
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Measurement of surface concentration of fluorophores using fluorescence fluctuation spectroscopy
... Fluorescence correlation spectroscopy (FCS) consists in calculating the autocorrelation of the fluorescence intensity time trace emanating from the small confocal volume illuminated by a ...
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Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell.
... INTRODUCTION Fluorescence microscopy techniques are commonly used to monitor protein-protein interactions in living cells taking advantage of the detection of Förster Resonance Energy Transfer (FRET) (Förster, ...
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Measuring, in solution, multiple-fluorophore labeling by combining Fluorescence Correlation Spectroscopy and photobleaching
... count rates per molecule of the long and short native strands is about twice that of free Alexa,. while that of denaturated short cDNA strands is close to Alexa[r] ...
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Deconstructing the room-temperature emission spectra of nanocrystals using Photon-Correlation Fourier Spectroscopy
... applications such as biological imaging, 21 displays, 24 photodetectors, 25 solar cells, 26 solid-state lighting, 27 and more. 1, 28 The growing use of nanocrystals in optical applications has relied on the interplay ...
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Direct solid surface fluorescence spectroscopy of standard chemicals and humic acid in ternary system
... Its fluorescence quantum yields is higher than the phenylalanine and tyrosine ones ...in fluorescence domain [18], particularly in environmental purpose where it is used to make the Quinine Sulfate Unit ...
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Detection of lead in brass by laser-induced breakdown spectroscopy combined with laser-induced fluorescence
... One promising avenue for improving the sensitivity of LIBS consists in using the LIBS-LIFS approach (LIBS combined with Laser-Induced Fluorescence Spectroscopy) [4]. It has already been shown for various ...
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Excited-state dynamics of dGMP measured by steady-state and femtosecond fluorescence spectroscopy.
... the spectroscopy of the monomeric DNA ...absorption spectroscopy, 6-12 fluorescence upconversion, 13-20 fluorescence Kerr gating 21,22 and time- resolved infrared spectroscopy 23 have ...
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Spectral Behavior of White Pigment Mixtures Using Reflectance, Ultraviolet-Fluorescence Spectroscopy, and Multispectral Imaging
... multispectral fluorescence imaging, we have chosen the filters, centered at 420 nm and 470 nm, that correspond to the emission band of the titanium white samples and the dried lead white in oil (Figure ...the ...
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Non-negative Matrix Factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing
... Keywords: Fluorescence spectroscopy, autofluorescence, blind source separation, Fluorescent Diffuse Optical Tomography ...back fluorescence is analyzed to localize the fluorescence ...
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