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DNA extraction

Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA

Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA

... Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S ...soil DNA extraction procedure Text (2500 characters, including ...

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A quick and cheap new DNA extraction method from lymnaeids snails.

A quick and cheap new DNA extraction method from lymnaeids snails.

... The DNA of one hundred Galba truncatula collected in july 2007 in the region of Boutheldja (Algeria) was extracted following two DNA extraction methods: -Classical phenol/chloroform-based DNA ...

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CENTRIFUGAL LABTUBE FOR FULLY AUTOMATED DNA EXTRACTION & LAMP AMPLIFICATION BASED ON AN INTEGRATED, LOW-COST HEATING SYSTEM

CENTRIFUGAL LABTUBE FOR FULLY AUTOMATED DNA EXTRACTION & LAMP AMPLIFICATION BASED ON AN INTEGRATED, LOW-COST HEATING SYSTEM

... isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA-extraction platform ...closed extraction of as little as 100 DNA copies of verotoxin-producing (VTEC) Escherichia ...

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Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

... Soil DNA and RNA, Quantitative co-extraction, Molecular biomass, Tropical soil Article history: Received 8 January 2015; Accepted 26 March 2015 ...commercial DNA extraction kit (FastDNA SPIN ...

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Optimization of DNA Extraction and PCR Conditions for Genetic Diversity Study in Artemisia HerbaAlba from Algeria

Optimization of DNA Extraction and PCR Conditions for Genetic Diversity Study in Artemisia HerbaAlba from Algeria

... a degradation of its natural habitat. It is necessary to constitute a collection in order to reintroduce it in the highlands of North Africa. For this purpose the genetic characterization is obviously important. ...

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Optimized microbial DNA extraction from diarrheic stools.

Optimized microbial DNA extraction from diarrheic stools.

... prevent DNA hydrolysis on human non-diarrheic fecal samples ...in DNA recovery com- pared to fresh stool specimens ...to DNA extraction increased the sensitivity of real-time PCR-based ...

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Molecular forensics in the precious Mediterranean red coral, Corallium rubrum: testing DNA extraction and microsatellite genotyping using dried colonies

Molecular forensics in the precious Mediterranean red coral, Corallium rubrum: testing DNA extraction and microsatellite genotyping using dried colonies

... until DNA extraction (reference colony) and the other part conserved at room temperature during 8 months (dried ...genomic DNA was extracted with a salting out procedure (Miller et ...extracted ...

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Limits of Ancient DNA Extraction from Teeth: The Case of Sudanese Nubia

Limits of Ancient DNA Extraction from Teeth: The Case of Sudanese Nubia

... for DNA extraction correlates negatively with closeness to the equator, and that no DNA older than 5000 years has been successfully amplified at latitudes lower than 30 ...extract DNA in ...

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A semi-automated protocol for Archaea DNA extraction from stools.

A semi-automated protocol for Archaea DNA extraction from stools.

... archaeal DNA, but also decrease the effects of PCR inhibitors ...automated DNA extraction, thus significantly decreasing the protocol turn-around time and rendering archaeal DNA extrac- tion ...

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A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete

A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete

... genomic DNA (gDNA) with high quality and integrity adapted to the organism of ...gDNA extraction from spores of Plasmopara halstedii, an obligate biotroph oomycete pathogen responsible for downy mildew in ...

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A Simple And Rapid Protocol Of Crude Dna Extraction From Apple Trees For Pcr And Real-Time Pcr Detection Of 'Candidatus Phytoplasma Mali'

A Simple And Rapid Protocol Of Crude Dna Extraction From Apple Trees For Pcr And Real-Time Pcr Detection Of 'Candidatus Phytoplasma Mali'

... 2.4. Phytoplasma detection by end-point and real-time PCR Specific primers AP5/AP4 designed by Jarausch et al. (1994) were used to check crude extract preparations as template for phyto- plasma detection by end-point PCR ...

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Biotechnological applications of the sepiolite interactions with bacteria: Bacterial transformation and DNA extraction

Biotechnological applications of the sepiolite interactions with bacteria: Bacterial transformation and DNA extraction

... sepiolite and DNA binding capacities can be used to purify plasmids from bacteria, 45.. representing an advantageous alternative to onerous commercial kits.[r] ...

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Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA

Influence of Soil DNA Extraction Procedure to Assess Bacterial Diversity using Pyrosequencing of 16S rDNA

... soil DNA was extracted from ...crude DNA extract was purified by using the Geneclean® Spin® Kit (MP ...Metagenomic DNA was used as target for PCR-based detection of IncP-1 backbone ...

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Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood

Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood

... of DNA Extraction Methods in Isolating Candida DNA from Spiked Blood All negative control whole blood samples (n = 55) remained ...automated DNA extraction of a range of yeast ...

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DNA nano-engineering and DNA driven nanoparticle assembly

DNA nano-engineering and DNA driven nanoparticle assembly

... 5.1.2 Functionalization protocol and colloidal characterization The overall DNA functionalization procedure, which is conceptually equivalent for both the Al and CuO nanoparticles, is shown in Figure 14. The first ...

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DNA Fluorescence

DNA Fluorescence

... to DNA one usually refers to fluorescent labels used extensively in biology and ...Yet DNA does emit fluorescence albeit with a low quantum yield, of the order of 10 -4 ...

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Extraction d'Information Non Supervisée à Partir de Textes – Extraction et Regroupement de Relations entre Entités

Extraction d'Information Non Supervisée à Partir de Textes – Extraction et Regroupement de Relations entre Entités

... CHAPTER 4. RELATION CLUSTERING than hierarchical ones from the viewpoint of computational complexity since no complete cluster hierarchy structures are attempted to be created. K-means clustering aims at separating data ...

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QUANTITATIVE ASSAY OF RNA AND DNA BY THE DOUBLE FLUOROCHROME METHOD : EXTRACTION OPTIMIZATION AND PIGMENT ALGAL INTERFERENCE

QUANTITATIVE ASSAY OF RNA AND DNA BY THE DOUBLE FLUOROCHROME METHOD : EXTRACTION OPTIMIZATION AND PIGMENT ALGAL INTERFERENCE

... fluorescence decreased regularly, even reaching négative values. The PE range was limited to concentrations which led to fluorescence intensi- ties similar to those [r] ...

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Feature extraction and signal processing for nylon DNA microarrays.

Feature extraction and signal processing for nylon DNA microarrays.

... Scanners have a limited measurement range which a par- ticular signal may exceed. This results in a saturation effect whereby some features are underestimated by conven- tional extraction methods [19], see Figure ...

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Combining C-value and Keyword Extraction Methods for Biomedical Terms Extraction

Combining C-value and Keyword Extraction Methods for Biomedical Terms Extraction

... Exemples C-value TFIDF, Okapi Table 1: Differences between ATR and AKE. In our work, we adopt as baselines an ATR method, C-value (Frantzi et al., 2000), and the best two AKE methods (Hussey et al., 2012), pre- viously ...

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