Citrus x aurantium L

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Evaluation des activités antibactérienne et antioxydante des huiles essentielles de Citrus aurantium et Citrus reticulata

Evaluation des activités antibactérienne et antioxydante des huiles essentielles de Citrus aurantium et Citrus reticulata

Mots clés : huiles essentielles, Citrus aurantium, Citrus reticulata, activité antibactérienne, activité antioxydante. Abstrat The objective of our work is to evaluate the antibacterial and antioxidant effect of the essential oils of Citrus aurantium and Citrus reticulata. The antibacterial activity was evaluated by the well diffusion technique on Muller Hinton medium on four strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeroginosa and Proteus vulgaris). The essential oil of Citrus reticulata revealed an interesting activity compared to Citrus aurantium with zones of inhibitions which vary between 3 and 25mm. Citrus aurantium was characterized by a weak zone of inhibition with a maximum of 12mm. Of all the bacteria tested, S. aureus was the one that showed the highest sensitivity. MICs were determined for both essential oils by the solid-state dilution method. Citrus reticulata showed excellent antibacterial activity compared to Citrus aurantium. Proteus vulgaris was the most sensitive bacterium with a MIC of 4μl / ml followed by Staphylococcus aureus (10μl / ml), Pseudomonas aeroginosa (16μl / ml) and Escherichia coli (18μl / ml). In contrast, Citrus aurantium was characterized by low antibacterial activity resulting in MIC values greater than 20μl / ml.
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Chemical Composition and in vitro Antimicrobial and Antioxidant Activities of Citrus aurantium L. Flowers Essential Oil (Neroli Oil)

Chemical Composition and in vitro Antimicrobial and Antioxidant Activities of Citrus aurantium L. Flowers Essential Oil (Neroli Oil)

s ectrome INTRODUCTION Citrus aurantium L., commonly named sour or bitter orange is a tree that belongs to the Rutaceae family. In Tunisia, C. aurantium L. is grown in warm regions shielded from strong winds, mainly in Cap Bon region which covers an area of 1500 ha. Sour orange trees are very resistant to cold, water excess and several diseases. For these main qualities, sour orange is widely used as a rootstock (Lota et al., 2001 ).

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ETUDE IN VITRO ET IN VIVO DES POTENTIALITES BIO-PESTICIDES DE L’EXTRAIT DE CITRUS AURANTIUM AMARA

ETUDE IN VITRO ET IN VIVO DES POTENTIALITES BIO-PESTICIDES DE L’EXTRAIT DE CITRUS AURANTIUM AMARA

I.2.1. Pouvoir insecticide in vitro : Les résultats du test d’efficacité d’extrait de la plante Citrus aurantium amara sur les adultes du puceron noir de la fève Aphis fabae (in vitro) sont très prometteurs (en annexesIII). Les variations de mortalité en fonction des doses montrent clairement l’efficacité des concentrations choisies. Dès le premier jour, on peut voir l’effet dose-réponse c’est-à-dire une différence de niveau de sensibilité des pucerons en fonction des doses appliquées. D’une manière générale et comparé au témoin (eau distillée), toutes les concentrations testées ont montrés une activité insecticide marquée.
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Phenolic contents and antioxidant activity of orange varieties (Citrus sinensis L. and Citrus aurantium L.) cultivated in Algeria: Peels and leaves

Phenolic contents and antioxidant activity of orange varieties (Citrus sinensis L. and Citrus aurantium L.) cultivated in Algeria: Peels and leaves

Phenolic contents and antioxidant activity of orange varieties (Citrus sinensis L. and Citrus aurantium L.) cultivated in Algeria: Peels and leaves.. Authors.[r]

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Etude phytochimique et l’activité antioxydante et antibactérienne de trois plantes de la famille de Rutaceae (Zanthoxylum armatum, Ruta graveolens et Citrus aurantium)

Etude phytochimique et l’activité antioxydante et antibactérienne de trois plantes de la famille de Rutaceae (Zanthoxylum armatum, Ruta graveolens et Citrus aurantium)

graveolens, and Citrus aurantium) that were selected to estimate their levels of secondary metabolites (phenolic compounds, flavonoids) and their biological activities. According to the work consulted, the results of phytochemical screening of the different plants reveal the presence of some common constituents namely flavonoids, saponosides, alkaloids, tannins and saponin. However it was reported that no coumarin was isolated and identified in the extract of R. graveolens. While, vitamins and minerals are among the various chemical components of C. aurantium. The assay of polyphenols and flavonoids shows that the three species contain high levels of these phenolic compounds. Antioxidant activity was significant for all three varieties studied. The antimicrobial test showed that the antimicrobial power of the extracts was very important and varied depending on the microorganism and the concentration. On the other hand the Gram- bacteria were more insensitive than the Gram + especially for the extract of C. aurantium.
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Evaluation de l’activité antioxydante de l’huile essentielle des feuilles de l’oranger amère (Citrus aurantium) seule et combinée avec la vitamine E

Evaluation de l’activité antioxydante de l’huile essentielle des feuilles de l’oranger amère (Citrus aurantium) seule et combinée avec la vitamine E

Le rendement en % Les feuilles séchées 1157 g 0.8 g 0.06 % Les feuilles fraiches 1157 g 3 g 0.2 % Le rendement en huile essentielle extraite à partir des feuilles fraiches de Citrus aurantium est de 0.2 %, Celle de l’HE extraite à partir des feuilles séchées de la même plante est de 0.06 %. Le rendement en huile essentielle des feuilles fraiches est plus important que celui de feuilles séchées, plusieurs travaux démontrées que le séchage de la plante à des effets très nets sur le rendement qui commence a augmenté jusqu’atteinte un maximum puis il baisse régulièrement. Sachant que la plante après sa récolte continue à vivre et son activité de biosynthèse des terpènes et dérivés s’accentue parce qu’elle est considère comme moyen de défonce contre le stresse hydrique, C’est ce qui explique l’augmentation des rendements en huiles essentielles à l’état fraiche. Après le séchage la plante meurt définitivement et ces activités de biosynthèse s’arrêtent et les huiles essentielles se perdent par évaporation, C’est ce qui explique la baisse de rendement (Bencheikh et al., 2015).
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etude du suivi des étapes de greffage du citrus clementina sur citrus aurantium

etude du suivi des étapes de greffage du citrus clementina sur citrus aurantium

Dans la région de Khenchela la plantation des bulbes se fait mécaniquement, par une machine spéciale, par ce qu’elle est la plus grande exploitation du safran en Algérie, de plus de 12 [r]

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The Distribution of Coumarins and Furanocoumarins in Citrus Species Closely Matches Citrus Phylogeny and Reflects the Organization of Biosynthetic Pathways

The Distribution of Coumarins and Furanocoumarins in Citrus Species Closely Matches Citrus Phylogeny and Reflects the Organization of Biosynthetic Pathways

select mandarins and Ichang papeda as Citrus varieties for use in creating species devoid of these toxic compounds in future breeding programs. Introduction Citrus originate from the tropical and subtropical regions of Southeast Asia and their culture was initiated in India and China during the first millenary BC [ 1 ]. Currently, citrus are culti- vated between the latitudes 40°N and 40°S in tropical and subtropical regions [ 2 ]. In 2013, cit- rus crops represented the most important fruit produced in the world, with more than 135 million tons [ 3 ]. Citrus belong to the Rutaceae family, which is composed of approximately 160 genera and 1900 species [ 4 ]; true citrus belong to the Aurantioideae subfamily, the Citreae tribe and the Citrineae subtribe. True citrus include 6 genera: Citrus, Fortunella, Poncirus, Microcitrus, Eremocitrus and Clymenia [ 5 ]. Citrus taxonomy remains controversial due to a long cultivation history, complex reproductive biology and somatic bud mutation. Swingle and Reece (1967) [ 5 ] and Tanaka (1977) [ 6 ], which recognize 16 and 162 species, respectively, remain the two major classification systems currently used. However, this last decade, molecu- lar analyses have provided decisive information regarding Citrus domestication and the rela- tions between various cultivated species of Citrus [ 7 – 14 ]. Four ancestral taxa, Citrus medica L. (citron), Citrus reticulata Blanco (mandarin), Citrus maxima (Burm.) Merr. (pummelo), and Citrus micrantha Wester (papeda), have been identified as the ancestors of all cultivated Citrus [ 8 , 10 , 12 , 14 ]. Among the four basic horticultural groups, no evidence of interspecific introgres- sion was found in pummelos, citrons, and C. micrantha. In contrast, evidence of introgression by C. maxima was found in sweet mandarin by sequencing and resequencing [ 13 , 14 ]. However, ‘Cleopatra’, ‘Sunki’ and ‘Shekwasha’ mandarin, which share the acidic mandarin cytoplasm defined by Froelicher et al. (2011) [ 10 ] from mitochondrial data and confirmed by Carbonell- Caballero et al. (2015) [ 15 ] with chloroplast data, present no evidence of introgression [ 14 ]. The secondary species Citrus sinensis (L.) Osb. (sweet orange), Citrus aurantium L. (sour orange), Citrus paradisi Macf. (grapefruit), Citrus limon (L.) Burm. (lemon), and Citrus auran- tifolia (Christm.) Swing. (lime) arose from the hybridization of the 4 ancestral taxa
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Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping

Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping

In citrus, the number of published markers of genomic SSRs is still limited [11,12]. Those markers were used for genetic diversity assessment and for germplasm manage- ment [13,14]. A high-density microsatellite consensus map is still lacking. The major goal of genetic mapping is to localize genes or QTLs, involved in traits of interest that are linked to molecular markers. Those molecular markers can be used as a starting point for gene identification or to reduce schemes of selection. One other way to address this aim is to develop markers directly localized in the coding sequences. ESTs (Expressed Sequence Tags) derived from cDNA libraries obtained from the genome expression have been investigated for microsatellite screening, in barley [15], wheat [16], rice [17], citrus [18,19], sugarcane [20] and grape [21]. It is assumed that those SSRs markers should enable to assess the molecular evolution of the genes in which they are positioned. Indeed, it has been observed that in ESTs, the flanking region of SSRs are more conserved and can also be found in related genera [22]. Thousands of EST-SSRs were iden- tified in numerous species such as grape and cereal. A high level of transferability was noted between rice, wheat and barley [17]. In citrus, thousands of ESTs are now available in databases. Recently, using public sequence databases resources, Chen et al. [23], published the characterization of 56 EST-SSR markers identified among 2295 citrus ESTs, mappable in a progeny obtained from a cross between sweet orange (Citrus sinensis L. Osb.) and trifoliate orange (Poncirus trifoliata L. Raf.). If those two genotypes repre- sent important resources of agronomical characters for rootstock and cultivar improvement scheme, numerous other citrus species offer a large panel of specific traits interesting breeders or consumers. For example, Clemen- tine (Citrus clementina Hort. Ex Tan.) is a model citrus crop in Mediterranean area and sour orange (C. aurantium L.) or Cleopatra mandarin (C. reshni Hort. Ex Tan.) are toler- ant to abiotic constraints such as salt stress or calcareous soils [24]. Citrus as many fruit trees have a juvenility period with around 5 years of duration limiting the possi- bility to study the allelic segregation on a second genera- tion of hybrids (F2 or BC). Consequently citrus genetic
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Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition

Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition

C. hamlin 95.78±0.82 a 149.83±5.03 f 05.99±0.20 e C. aurantium 68.44±3.71 c 353.48±3.81 b 13.34±0.11 a C. grandis 94.63±1.26 a 167.59±3.14 e 07.19±0.18 d Values are mean±standard deviation (n=3). Means followed by the same letter are not di昀ferent according to ANOVA Analysis of variance. ABTS, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; DPPH, 2,2-diphenyl picrylhydrazyl; IC 50 , e昀fective concentration 50 %; TE, Trolox equivalents; BHAE,

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Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition

Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition

C. hamlin 95.78±0.82 a 149.83±5.03 f 05.99±0.20 e C. aurantium 68.44±3.71 c 353.48±3.81 b 13.34±0.11 a C. grandis 94.63±1.26 a 167.59±3.14 e 07.19±0.18 d Values are mean±standard deviation (n=3). Means followed by the same letter are not di昀ferent according to ANOVA Analysis of variance. ABTS, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; DPPH, 2,2-diphenyl picrylhydrazyl; IC 50 , e昀fective concentration 50 %; TE, Trolox equivalents; BHAE,

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Évaluation de l’effet du stress hydrique et du portegreffe sur la clémentine Citrus reticulata Swingle var. Sidi Aissa.

Évaluation de l’effet du stress hydrique et du portegreffe sur la clémentine Citrus reticulata Swingle var. Sidi Aissa.

Objective: The aim of this study is to compare the morphological and physiological response of young Sidi Aissa Clementine trees grafted onto five citrus rootstocks and cultivated under two different water regimes in order to select rootstocks with a height potential to mitigate drought conditions. Materials and methods: The experiment was carried out in the greenhouse of the experimental farm of El Menzh INRA-Kenitra, Morocco on one year plastic potted (10 L) Sidi Aissa Clementine trees grafted onto five different rootstocks namely le Citrus volkameriana, Citrus macrophylla, Citrange C35 (Citrus sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.), Citrange Carrizo (Citrus sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.) and hybrid of Mandarin Cléopâtre (Citrus reticulata var . Austera Swingle) X Citrange Carrizo 30577 [C. sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.) . Water regimes applied were 50% and 100% H cc (humidity at field capacity) of the
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Study of tolerance to natural chilling stress in triploid citrus

Study of tolerance to natural chilling stress in triploid citrus

Seedlessness is one of the most consumer expectation on the fresh-fruit market. Polyploidy is an alternative developed to improve tolerance to abiotic stresses. However, little is known about the response of triploid citrus to low temperatures. The creation of triploid hybrid citrus is an alternative to develop innovative seedless commercial varieties, which present a greater abiotic stress tolerance. MATERIALS AND METHODS

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Tetraploid Citrumelo 4475 (Citrus paradisi L. Macf. × Poncirus trifoliata L. Raf.) Rootstocks Improve Common Clementine Tolerance to Long-term Nutrient Deficiency (Citrus clementina Hort. ex Tan)

Tetraploid Citrumelo 4475 (Citrus paradisi L. Macf. × Poncirus trifoliata L. Raf.) Rootstocks Improve Common Clementine Tolerance to Long-term Nutrient Deficiency (Citrus clementina Hort. ex Tan)

Many Citrus genotypes are used as rootstock for citrus cultivation. Genotypes belong either to the Citrus genus such as Volkamer lemon or are obtained by hybridization between Citrus and Poncirus genus progenitors such as Citrumelo 4475 and Carrizo citrange. Volkamer lemon which is used as rootstock for lemon, is adapted to dry, calcareous and saline soils and presents tolerance to Tristeza, cachexia and exocortis. Citrumelo 4475 imparts cold tolerance to the scion. Citrange Carrizo is frequently used in acidic and neutral soils but not in dry areas because of its limited performance under drought conditions. These two genotypes inherited Tristeza tolerance from their Trifoliate orange progenitor and give clementine varieties that produce a high yield and fruit quality 19 .
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Ni 1‐x Co x O y , Ni 1‐x Co x S y and Ni 1‐x Co x P y Catalysts Prepared from Ni 1‐x Co x ‐ZIF‐67 for Hydrogen Production by Electrolysis in Alkaline Media

Ni 1‐x Co x O y , Ni 1‐x Co x S y and Ni 1‐x Co x P y Catalysts Prepared from Ni 1‐x Co x ‐ZIF‐67 for Hydrogen Production by Electrolysis in Alkaline Media

dodecahedral shape with an average size of 300 nm, while the Ni 0.2 Co 0.8 O y crystals have an average size of 206 nm (Fig. 2d). The crystal size of the samples increases about twice after oxidation of the Ni x Co 1- x -ZIF-67, indicating that two or three Ni x Co 1-x -ZIF-67 nanoparticles coalesce into one Ni x Co 1-x O y particle. In the case of phosphides, the derived CoP showed an average size of 34 nm (Fig. 2e), and the N i0.1 Co 0.9 P y

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$M(x)=o(x)$ Estimates for Beurling numbers

$M(x)=o(x)$ Estimates for Beurling numbers

de Bordeaux 30 (2018), 469–483 M (x) = o(x) Estimates for Beurling numbers par Gregory DEBRUYNE, Harold G. DIAMOND et Jasson VINDAS Résumé. Dans la théorie des nombres premiers classique, certaines ex- pressions sont considérées comme « équivalentes » au TNP (Théorème des nombres premiers). Parmi elles on trouve la borne M (x) = o(x) pour la fonc- tion sommatoire de Moebius. Dans le cas des nombres premiers, généralisés par Beurling, cette borne ne suit pas nécessairement du TNP sans exiger des hypothèses additionelles. Ici, deux conditions sont présentées, impliquant la version Beurling pour la borne sur M (x) et quelques exemples sont construits, démontrant l’absence éventuelle de cette borne si ces conditions ne sont pas réalisées.
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Influence of mitochondria origin on fruit quality in a citrus cybrid.

Influence of mitochondria origin on fruit quality in a citrus cybrid.

KEYWORDS: Citrus; diploid somatic hybrid; cybrid; sugars; organic acids; carotenoids; fruit quality INTRODUCTION Nutritional and organoleptic fruit qualities are currently very important objectives for plant breeders. For citrus cultivars, fruit quality must be managed at the same time as other characters, such as seedlessness, productivity, harvesting period, and sometimes tolerance to diseases (1). Moreover sexual breeding of citrus is a difficult task mostly because of the highly heterozygous genetic structure of modern species, including sweet orange, grapefruit, and lemons, that are the result of interspecific hybridization (2). For these species, breeding methods are needed that do not affect the complex genetic structures that define the pomological and organoleptic char- acteristics of the species (3).
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Detection of Citrus Psorosis Virus Using an Improved One-Step RT-PCR

Detection of Citrus Psorosis Virus Using an Improved One-Step RT-PCR

After CPsV sequences became available, reliable systems were designed to detect CPsV by conventional RT-PCR. This research aims firstly to establish and compare different proto- cols for nucleic acid extraction from citrus tissues, to deter- mine their effectiveness in relation to their sensitivity and costs, to find specific primers for Moroccan psorosis isolates between selected primers pairs, and finally, to compare the results with those of DAS-ELISA and biological assay.

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Cytological and molecular characterization of three gametoclones of Citrus clementina

Cytological and molecular characterization of three gametoclones of Citrus clementina

Methods Plant material Three gametoclonal plants, designated ESP, FRA, and ITA respectively acquired in the lab of Navarro (Spain), Ollitrault (France), and Germanà (Italy), were all derived from Citrus clementina Hort. ex Tan., cv. Nules and pre- liminarily shown to be homozygous based on some se- lected loci. They were obtained by in situ parthenogenesis induced by irradiated pollen followed by in vitro embryo culture, or by pollen embryogenesis. Specifically, ESP was through in vivo-induced gynogenesis by pollination of Nules Clementine with irradiated pollen of Fortune man- darin followed by in vitro embryo rescue [22], FRA also through gynogenesis by pollination in the field with irradi- ated Meyer lemon (Citrus meyeri Y. Tan.) pollen [11], and ITA was obtained through anther culture of C. clementina cv. Nules [15,17]. ESP was previously characterized as a haploid [22]. All three plants were much less vigorous than the heterozygous mother plant, as revealed by leaf size and growth habit (Additional file 1: Figure S1). Sam- ples from all three plants were sent to the respective la- boratories of the collaborators for the specific analyses to which each group had committed.
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Nutrient Deficiency Tolerance in Citrus Is Dependent on Genotype or Ploidy Level

Nutrient Deficiency Tolerance in Citrus Is Dependent on Genotype or Ploidy Level

MATERIALS AND METHODS Plant Material and Growth Conditions The experiment was performed on four citrus diploid (2x) seedling genotypes and their four doubled diploid (4x) counterparts (Table 1). The Flhorag1, an allotetraploid form, was also included. Plants were selected among seedlings made with seeds from trees maintained in the citrus germplasm collection (BCR NF 96-S-900 Citrus INRA/CIRAD) at San-Giuliano, Corsica (France). The ploidy status of 2x and 4x seedlings was checked by 10-color flow cytometry (Partec I, Germany) as described by Froelicher et al. (2007) . Clonal propagation by nucellar embryogenesis was verified by genotyping using SSR markers as described by Vieira et al. (2016) . The 12 seedlings of each genotype, giving a total of 108 plants were grown under identical conditions in vermiculite with ferti-irrigation and water (1 L/h) during 4 years in a tunnel greenhouse at the AREFLEC experimental station in San-Giuliano, Corsica (41 ◦
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