Budding yeast
Budding yeast 14-3-3 proteins contribute to the robustness of the DNA damage and spindle checkpoints
... the budding yeast Saccharomyces cerevisiae, are Mec1, Mec3, Rad53 and Chk1, and the spindle checkpoint, which comprises the two independent Bub2 and Mad2 ...
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DNA replication in budding yeast : link between chromatin conformation and kinetics of replication
... Concerning the hypothesis that the peak is created by ribosomes aggregates, the conclusion is more difficult. Indeed, ribosomes biogenesis is well documented (Woolford and Baserga 2013) but its kinetics during the cell ...
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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
... The software was intended to be flexible for analysis of various time-lapse fluorescence microscopy data. Any number of color channels can be included and any may be used to create a sub-region mask. While the algorithms ...
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Long-term tracking of budding yeast cells in brightfield microscopy: CellStar and the Evaluation Platform
... In the following, we focus on the analysis of microscopy images of the budding yeast Saccharomyces cerevisiae observed in brightfield. While fluorescent markers can be used to tag cellular compartments and ...
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Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast
... DISCUSSION The accumulation of protein aggregates is often attributed to a decline of protein folding quality control functions in the cell ( Nystro¨m, 2011 ; Treusch et al., 2009 ; Winkler et al., 2012 ). Asym- metric ...
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Physical principles and functional consequences of nuclear compartmentalization in budding yeast
... Silencing foci: Unlike other eukaryotes containing centromeric heterochromatin, budding yeast chromosomes have very little repetitive sequences outside of the rDNA. Transcriptionally inert and compacted ...
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Beyond hairballs: depicting complexity of a kinase-phosphatase network in the budding yeast
... the budding yeast assembled in this thesis is, to the best of our knowledge, the first and largest assembled network containing both bona fide PDIs, proposing the KP-Net as a reliable gold standard for ...
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Rvb2/reptin physically associates with telomerase in budding yeast
... fission yeast Schizosaccharomyces pombe (4), while in the budding yeast Saccharomyces cerevisiae, a somewhat simpler protection complex, consisting mainly of the Cdc13, Stn1 and Ten1 proteins ...
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en fr Mechanisms of asymmetrical segregation of Hsp104-bounded protein aggregates in budding yeast Mécanismes de ségrégation asymétrique des agrégats protéiques liés à Hsp104 chez Saccharomyces cerevisiae
... Je remercie chaleureusement les membres actuels et passés du laboratoire. Steffen, avec qui j’ai eu l’occasion d’initier ce travail, Youlian, roi de la statistique, Cecilia, dont l’arrivée au sein du labo a sans aucun ...
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Cell cycle regulation during gametogenesis in budding yeast
... Furthermore, it bypasses the metaphase I delay in rec8-29A cells because preventing recombination prevents the formation of linkages between homologous chromosomes and hence th[r] ...
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A mechanism for co-transcriptional recruitment of mRNA localization factor on nascent mRNAs in budding yeast
... that non-classical NLS of She2p is present between the amino acids 214-222 at the C- terminal end of this protein, and that mutation of five specific residues in this NLS disrupts the nuclear targeting of She2p The ...
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Budding yeast cell cycle analysis and morphological characterization by automated image analysis
... This response is different depending on a given yeast cell's current stage in the cell cycle, so it is important to be able to determine that piece of information [r] ...
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YeastNet: deep-learning-enabled accurate segmentation of budding yeast cells in bright-field microscopy
... track yeast cells in a series of images of monolayer colonies acquired by time-lapse microscopy, and is initiated by two GUIs, CTseed and CTtrack, in MATLAB (Mathworks ...
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Phosphoproteomic Analysis of Isolated Mitochondria in Yeast
... 7. Amoutzias, G.D., He, Y., Lilley, K.S., Van de Peer, Y. and Oliver, S.G. (2012) Evaluation and Properties of the Budding Yeast Phosphoproteome. Molecular & Cellular Proteomics, 11, M111.009555. 8. ...
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Aneuploidy Drives Genomic Instability in Yeast
... 13 budding yeast strains that carry extra copies of single chromosomes and found that all aneuploid strains exhibited one or more forms of genomic ...fission yeast strains also exhibited defects in ...
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Severe osmotic compression of the yeast Saccharomyces cerevisiae
... and yeast are surrounded by highly resistant cell walls that mechanically protect them from bursting ...In yeast, osmotic shrinkage is completed within a few tens of seconds after exposure to increased ...
197
Yeast-Based Biosensors: Current Applications and New Developments
... bakers’ yeast) was the first eukaryotic organism whose genome was entirely sequenced [7] and is remarkably easy to modify ...conserve yeast cells makes them an extremely interesting choice of biological ...
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Yeast RNase III triggers polyadenylation-independent transcription termination
... the yeast orthologue of the dsRNA-specific ribonuclease III (Rnt1p) may trigger Rat1p dependent termination of RNA transcripts that fail to terminate near polyadenylation ...
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10th Francophone Yeast Meeting 'Levures, Modèles & Outils'
... The yeast is an easy genetic tool allowing researchers to delve deeper into the understanding of complex mechanisms, particularly relevant for many aspects of the RNA life cycle that were developed in the ‘RNA, ...
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Influence of micro-environment on yeast population dynamics
... 4. RESULTS 4.1. Raw data on yeast population dynamics Yeast population dynamics were carried out for 12 concentration of glucose in g/L: 0, 0.1, 0.5, 1, 5, 10, 30, 50, 75, 100, 200, 300 over a time period ...
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