Bioluminescence resonance energy transfer
Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology
... The bioluminescence resonance energy transfer (BRET) approach involves resonance energy transfer between a light-emitting enzyme and fluorescent ...
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SUBCELLULAR IMAGING OF DYNAMIC PROTEIN INTERACTIONS BY BIOLUMINESCENCE RESONANCE ENERGY TRANSFER.
... appropriate energy acceptor such as the yellow fluorescent protein (YFP) is present within resonance energy transfer permissive distances of Rluc, part of the energy can be transferred ...
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Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET)
... As a positive control, a biotin-labeled 60 bp duplex bearing the Epstein-Barr Nuclear antigen 1 (EBNA-1) binding sequence was mutants excited by energy transfer (Em535) and BRET signal generated by the two ...
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Real-time analysis of agonist-induced activation of protease-activated receptor 1/Galphai1 protein complex measured by bioluminescence resonance energy transfer in living cells.
... to energy acceptor and donor ...the energy acceptor yellow fluorescent protein (YFP) and the energy donor luciferase from Renilla reniformis (Rluc), respectively ...
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Development of a human breast-cancer derived cell line stably expressing a bioluminescence resonance energy transfer (BRET)-based phosphatidyl inositol-3 phosphate (PIP3) biosensor.
... of Luc-Akt-PH to the plasma membrane results in an energy transfer between the luciferase and the YFP (BRET) that can be monitored in living cells cultured in 96 well plates (Fig. 1A). This assay ...
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Frontiers Commentary onTallet et al. Investigation of prolactin receptor activation and blockade using time-resolved fluorescence resonance energy transfer A commentary on Investigation of prolactin receptor acti- vation and blockade using time-resolved fluorescence resonance energy transfer
... using bioluminescence resonance energy transfer (BRET) and co-immunoprecipitation (co- IP) approaches, concluded that the PRLR constitutively homodimerize (or het- erodimerized when long and ...
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Long-Range Plasmon Assisted Energy Transfer Between Fluorescent Emitters
... of energy transfer through the SPP ...range energy transfer ...mediated energy transfer over distances of up to 7 µm is given by the graph shown in red in ...the energy ...
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Advanced Nuclear Energy Systems: Heat Transfer Issues and Trends
... nuclear energy systems a more attractive option for deployment around the ...nuclear energy systems must be able to be licensed, constructed, and operated in a manner that will provide a competitively ...
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la bioluminescence de l'aequorine en réponse au calcium In vitro et dans le Cortex cerebral
... [Ca 2⫹ ] range (⌺ F 兾⌺ T , 2.5 ⫾ 0.7% at 1.24 mM Ca 2⫹ ) suggest that, in contrast with EF3 ⫺ SloDK mutants, the participation of EF3 to bioluminescence was not abolished in the AHV mutant. These data confirm that ...
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Membranes: a variety of energy landscapes for many transfer opportunities
... The energy to overcome the barrier comes from the drag force (the permeate flux) that is transmitted on all the accumulated particles (the drag force must be multiplied by the number of accumulated layers or by ...
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Impact of the Langdon effect on crossed-beam energy transfer
... four thousand pairwise interactions that can induce a macroscopic redistribution of laser intensity within the hohlraum interior. The ability to manipulate this process via laser frequency detuning was initially seen as ...
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Energy transfer up-conversion in Tm3+-doped silica fiber
... 4 level was taken as the time required for the fluorescence intensity to decrease to 1/e of its peak intensity. This non-exponential behaviour has been observed in many other thulium doped glasses [5, 16, 17]. In many ...
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Numerical simulations of energy transfer in counter-streaming plasmas
... rest energy, ,the electric and magnetic fields is are in relativistic plasma units, , and , respectively. An experiment for studying the collisionnelss mechanism of fast ion energy in laboratory plasma is ...
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Viabilité des moisissures dans les taches de foxing/ dosage de l'ATP par bioluminescence
... Abstract Previous studies found evidence of mold-like structures in some colored areas on paper damaged by foxing; many species have not been identified and their role in the phenomenon is not yet understood. In order to ...
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Using Targeted Energy Transfer to Stabilize Drill-string Systems
... nonlinear energy sink (NES) , an attachment which has been shown to be effective in reducing or even eliminating self-excited motions in van der Pol and aeroelastic ...vibrational energy from primary ...
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Nonlinear energy transfer to improve the acoustic black hole effect
... the wedge profile where the thickness is decreasing, the amplitude of the vibrations becomes rapidly of the same order as the thickness, hence exciting geometric nonlinearity. The device can then be adapted by using a ...
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Influence of spin-transfer torque on thermally activated ferromagnetic resonance excitations in magnetic tunnel junctions
... this paper, we present a detailed analysis of this phenom- enon. In Sec. II, an analytical macrospin model is developed in the framework of the linear-response theory. First we cal- culate the magnetic noise power ...
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Membranes: A Variety of Energy Landscapes for Many Transfer Opportunities
... The energy to overcome the barrier comes from the drag force (the permeate flux) that is transmitted on all the accumulated particles (the drag force must be multiplied by the number of accumulated layers or by ...
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Humic Substances Enhance Chlorothalonil Phototransformation via Photoreduction and Energy Transfer.
... This work also stresses the importance of the nature of HS on both its reducing and sensitizing properties. Fulvic acids from Elliott as well as humic acids extracted from natural carbonaceous material are better ...
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Optimization mechanism of targeted energy transfer with vibro-impact energy sink under periodic and transient excitation
... For transient excitation, there does not exist SMR. Re- sponse regime continuously transits from one type to an- other type with the decrease of C. However, the transient response can be approximately described by the ...
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