A Combination of screening and computational approaches for the identification of novel compounds that decrease mast cell degranulation
78
0
0
Texte intégral
(2) $ (*+())), %#%!! $ $ # $" ' %" # . .
(3) . . . %!! $"& $ # $" # ! (.-5 &&-( (.# ) # - . (.#9#(#.,)*" (3& ;< ! ')() &)(& (.# ) 3 1- +/#, ,)' #)4)& ; ?EHBE<5 #(#.,)*" (3&9 )($/!. "/'( - ,/' & /'#( ;EEE@<5 ;AED?< ( ,#.)( 9@?? ;HAGC< 1 , ,)' #!'5 C9' ."3&/' &&# ,3&99 .3&9=99!&/ )-'#(# ;CFCD?A< ( 3% #("# #.),;DFCF@@< ,)'& #) " '8 AHC??A1- ,)' &&#!(&#(!8 9A B && &#( 1- */, "- ,)' ( !,)1( #( && /&./, ' # /**& ' (.- 1 , ) .#( ,)' (0#.,)! (( .& )0#( - ,/';@D9@D@<1- ,)' ),.),# -8 &
(4) 3).) &&- 1 , !,)1( #( #!" &/ )- ;# )< 1#." @?I ( * (# #&&#(:-., *.)'3 #(8 (.# ) # -1 , &&*/, "- ,)' &&#!(&#(!;*%.5CFB( B?G5.%.<8 ")-*". / , -&#( ;< ( @ 1 , *,)0# 3 ." #(9")/- - ,0# #&#.38 3,) >- / , ;3< )(-#-. ) #(#'/' -- (.#& #/' 1#.")/. " ()& ; (0#.,)! (< -/**& ' (. 1#."A' 9!&/.'#( ; (0#.,)! (<( A?' 89 1- #--)&0 .)@ '!:'& #( #&&#*), 1. ,8 -/ -.,. -)&/.#)( )(-#-. ) A8D ' C9' ."3&/' &&# ,3&99 .3&9β99!&/ )-'#(# #(?8?D #.,. 5* C8D6-.)*-)&/.#)(1-?8@ B:?8@AB5 * @?8 " ,). #( --3
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
(6) Supplementary Figure 1. Compounds reduced both T308 and S473 phosphorylation of Akt in unstimulated RBL-2H3 cells and there is no decrease in Akt phosphorylation in compounds that show no decrease in degranulation. A.. B.. Ě ƌ ƚĞ Ě ŶĞ ŝƚŽ Ğ ŝ ď ŝ ƚ Ɛ ŵ ƵůĂ ĞĨŽ 5Y2 7Y 9Y 5Y /ŶŚ Ɵ ƚ Ɛ ŵ ŝů 5 0 9 7 Ŭ hŶ ^Ɵ D J5 J6 J6 J7 ^LJ Ă Ƶů. -. +. - - - - - -. pAkt-T308 tAkt pAkt-S473 tAkt. C..
(7) Supplementary Table 1: Degranulation Assay Results and Safety Index Ranking compound J702Y J584E J555Y J521Y J676Y J597E J539C J589E J608E J647E J682Y J694Y J667Y J678Y J689Y J664Y J595E J475E J438Y J509Y J671Y J530Y J505Y J507Y J491C J532Y J607Y J541E J534E IBS-87 J655Y J656Y J500E J511Y J599E J546Y J623E J520Y J453E J660Y J573Y J670Y J553E J674Y. % control -1.8 0.3 1.0 1.4 1.4 1.4 1.9 2.0 2.0 2.1 2.7 2.9 3.1 3.1 3.4 3.4 4.0 4.0 4.2 4.3 4.7 4.7 4.9 4.9 4.9 5.0 5.0 5.2 5.3 5.4 5.5 5.5 5.9 6.2 6.3 7.0 7.0 7.1 7.2 7.2 7.5 7.8 7.9 8.2. % inhibition 101.8 99.7 99.0 98.6 98.6 98.6 98.1 98.0 98.0 97.9 97.3 97.1 96.9 96.9 96.6 96.6 96.0 96.0 95.8 95.7 95.3 95.3 95.1 95.1 95.1 95.0 95.0 94.8 94.7 94.6 94.5 94.5 94.1 93.8 93.7 93.0 93.0 92.9 92.8 92.8 92.5 92.2 92.1 91.8. at conc (µM) 25 25 25 10 25 25 10 25 25 25 25 25 25 25 25 25 10 25 15 25 25 10 25 25 25 25 25 25 10 25 25 25 25 25 25 10 25 25 25 25 25 25 25 25. J683Y. 8.6. 91.4. 25. J673Y J557E J680Y IBS-11 J590Y J663Y J620Y J626Y. 9.2 9.2 9.3 9.4 10.0 10.0 11.0 11.0. 90.8 90.8 90.7 90.6 90.0 90.0 89.0 89.0. 25 25 25 25 25 25 25 25.
(8) J524Y J693Y J699Y J550E J567E J575Y J614Y J637Y J528E J576E J549E J649Y J473C J545E J533Y J554Y J677Y Miltefosine J653Y J688Y J514E J686Y J606E J619Y J625E J714Y IBS-16 J661Y J588Y J629E J692Y J525E J448E J551E J566E J668Y J583E J601Y J627E J687Y J598E J697Y J585Y J538E J684Y J600E J622Y J444C J577E J479Y J645E J666Y J547E J578Y J610Y J644Y. 11.1 11.4 11.5 11.9 12.0 12.0 12.0 12.0 12.1 12.3 12.3 12.5 12.7 12.9 13.0 13.0 13.1 13.2 13.3 13.4 13.5 14.0 14.0 14.0 14.0 14.5 14.6 14.8 15.0 15.0 15.0 15.1 15.3 15.4 15.4 15.8 16.0 16.0 16.0 16.0 16.2 16.4 16.5 16.5 16.8 16.9 17.0 17.4 17.4 17.5 18.0 18.1 18.1 18.5 19.0 19.0. 88.9 88.6 88.5 88.1 88.0 88.0 88.0 88.0 87.9 87.7 87.7 87.5 87.3 87.1 87.0 87.0 86.9 86.8 86.7 86.6 86.5 86.0 86.0 86.0 86.0 85.5 85.4 85.2 85.0 85.0 85.0 84.9 84.7 84.6 84.6 84.2 84.0 84.0 84.0 84.0 83.8 83.6 83.5 83.5 83.2 83.1 83.0 82.6 82.6 82.5 82.0 81.9 81.9 81.5 81.0 81.0. 25 25 25 10 25 25 25 25 25 10 10 25 25 10 10 25 25 10 25 25 25 25 25 25 25 25 25 25 10 25 25 25 25 10 10 25 10 25 25 25 25 25 25 25 25 25 25 25 10 25 25 25 10 10 25 25.
(9) J669Y J651E J556Y J609E J616E J707Y J665Y J681Y IBS-68 J488Y J635E J720Y J429E J657E J632E IBS-63 J612C J703Y J558E J634E J522E J543Y J480Y J548Y J604E J569E J594E IBS-64 J675Y J621E J711Y J658E J477C J679Y J455Y J552E J709Y IBS-41 J484C IBS-15 J469Y IBS-105 J698Y J690C J615E J624E J672Y J712Y J628E J646E J706Y J718Y J633E J700E IBS-44 J705Y. 19.3 19.9 20.0 20.0 20.0 20.2 20.2 20.3 20.7 21.0 21.0 21.1 21.2 22.8 23.0 23.5 24.0 24.5 24.9 25.0 25.8 26.0 26.3 26.5 26.5 26.6 28.3 28.4 28.5 29.0 29.3 29.5 29.8 29.9 30.4 31.9 32.7 33.8 34.0 34.0 35.0 35.3 36.1 36.1 37.0 37.0 37.3 38.2 39.0 39.2 39.3 40.2 41.0 41.7 45.2 46.7. 80.7 80.1 80.0 80.0 80.0 79.8 79.8 79.7 79.3 79.0 79.0 78.9 78.8 77.2 77.0 76.5 76.0 75.5 75.1 75.0 74.2 74.0 73.7 73.5 73.5 73.4 71.7 71.6 71.5 71.0 70.7 70.5 70.2 70.1 69.6 68.1 67.3 66.2 66.0 66.0 65.0 64.7 63.9 63.9 63.0 63.0 62.7 61.8 61.0 60.8 60.7 59.8 59.0 58.3 54.8 53.3. 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 10 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25.
(10) IBS-65 J654Y J704Y IBS-23 IBS-83 J497Y J586E J462E J419E J562Y J431Y J485E IBS-101 J602E IBS-104 J715Y J640E J540C J641E J710Y J568E KA-4 J713Y IBS-42 J518Y J443E J460Y J708Y J648E IBS-66 J478C J636Y J652E J529E J603E J537E J685Y J592C J476S J719Y J650Y KA-5 J519Y KA-3 KA-2 J542E IBS-46 J696Y J531Y KA-52 J605Y J563Y IBS-17 J482C IBS-24 KA-10. 48.5 49.2 50.8 51.9 52.1 53.0 53.7 54.0 57.9 60.3 63.0 63.0 63.5 64.9 65.6 65.8 66.1 66.5 67.0 67.3 67.7 67.8 67.9 68.0 68.4 69.0 69.0 70.4 70.7 70.7 71.0 72.0 72.9 73.0 73.2 73.3 73.4 74.2 76.0 76.3 76.3 76.8 77.3 77.5 78.2 79.0 79.5 79.7 80.7 81.7 83.5 84.3 84.5 85.0 85.4 85.9. 51.5 50.8 49.2 48.1 47.9 47.0 46.3 46.0 42.1 39.7 37.0 37.0 36.5 35.1 34.4 34.2 33.9 33.5 33.0 32.7 32.3 32.2 32.1 32.0 31.6 31.0 31.0 29.6 29.3 29.3 29.0 28.0 27.1 27.0 26.8 26.7 26.6 25.8 24.0 23.7 23.7 23.2 22.7 22.5 21.8 21.0 20.5 20.3 19.3 18.3 16.5 15.7 15.5 15.0 14.6 14.1. 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25.
(11) J405S J565Y J461Y J506Y IBS-45 IBS-20 IBS-97 J593C J494E KA-7 J662C IBS-35 IBS-50 IBS-56 J570E IBS-79 KA-38 IBS-96 J643Y IBS-43 J596C J495Y KA-53 J572E J571E IBS-98 IBS-106 J510Y J428E J508Y KA-33 KA-6 KA-9 KA-32 KA-23 KA-31 KA-22 IBS-36 IBS-85 J580Y J454E J489Y IBS-30 J611Y J613C KA-12 J659Y IBS-58 J617E J503E J501E KA-50 KA-37 J439S KA-27 IBS-21. 85.9 86.0 87.0 87.0 87.1 87.1 87.6 87.8 88.6 89.4 89.5 89.5 89.5 89.7 90.0 90.1 90.2 90.2 90.7 90.7 90.9 90.9 90.9 91.0 91.1 91.1 91.5 91.7 91.9 92.5 92.6 92.7 93.0 93.2 93.5 93.5 93.7 94.0 94.4 94.4 95.0 95.0 95.1 95.1 95.2 95.4 95.5 95.9 96.0 96.0 96.3 96.9 96.9 96.9 97.0 97.8. 14.1 14.0 13.0 13.0 12.9 12.9 12.4 12.2 11.4 10.6 10.5 10.5 10.5 10.3 10.0 9.9 9.8 9.8 9.3 9.3 9.1 9.1 9.1 9.0 8.9 8.9 8.5 8.3 8.1 7.5 7.4 7.3 7.0 6.8 6.5 6.5 6.3 6.0 5.6 5.6 5.0 5.0 4.9 4.9 4.8 4.6 4.5 4.1 4.0 4.0 3.7 3.1 3.1 3.1 3.0 2.2. 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 100 100 25 25 25 25 25.
(12) IBS-2 J449E IBS-103 J582C J447C J430S J536Y J502E J564Y KA-30 J631Y J559S IBS-102 J486Y KA-36 J437Y J561Y IBS-49 IBS-100 KA-14 KA-25 J560S J639E KA-34 IBS-33 KA-29 J515Y IBS-47 J513E J591E KA-26 IBS-34 KA-28 KA-17 J459Y IBS-95 KA-13 J523E KA-24 KA-42 J483C IBS-107. 97.8 98.0 98.1 98.3 98.6 99.2 99.4 99.4 99.5 99.8 100.0 100.3 100.7 101.0 101.2 101.4 101.7 102.0 102.7 102.7 103.0 103.1 103.3 103.7 103.7 104.2 104.3 104.8 105.5 105.6 106.0 107.7 108.8 108.8 109.0 109.1 109.2 110.5 112.0 114.8 115.0 132.1. 2.2 2.0 1.9 1.7 1.4 0.8 0.6 0.6 0.5 0.2 0.0 -0.3 -0.7 -1.0 -1.2 -1.4 -1.7 -2.0 -2.7 -2.7 -3.0 -3.1 -3.3 -3.7 -3.7 -4.2 -4.3 -4.8 -5.5 -5.6 -6.0 -7.7 -8.8 -8.8 -9.0 -9.1 -9.2 -10.5 -12.0 -14.8 -15.0 -32.1. 25 25 25 25 25 25 25 100 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25 25.
(13) Safety Index Ranking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(14) .
(15) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(16) . . . . . . . . . . . . . . . . . . . .
(17)
(18)
(19)
(20)
(21)
(22)
(23)
(24)
(25)
(26)
(27)
(28)
(29)
(30)
(31)
(32)
(33)
(34)
(35)
(36)
(37)
(38)
(39)
(40)
(41)
(42)
(43)
(44)
(45)
(46)
(47)
(48) .
(49) . . . . . . . . . . . . . . . . . . .
(50)
(51)
(52)
(53)
(54)
(55)
(56)
(57)
(58)
(59)
(60)
(61)
(62)
(63)
(64)
(65)
(66)
(67)
(68)
(69)
(70)
(71)
(72)
(73)
(74)
(75)
(76)
(77)
(78)
(79)
(80)
(81)
(82)
(83)
(84)
(85)
(86)
(87)
(88)
(89)
(90)
(91)
(92)
(93)
(94)
(95)
(96)
(97)
(98)
(99)
(100)
(101)
(102)
(103)
(104) .
(105) . . . . . . . . . . . . . . . . . . . . .
(106)
(107)
(108)
(109)
(110)
(111)
(112)
(113)
(114)
(115)
(116)
(117)
(118)
(119)
(120)
(121)
(122)
(123)
(124)
(125)
(126)
(127)
(128)
(129)
(130)
(131)
(132)
(133)
(134)
(135)
(136)
(137)
(138)
(139)
(140)
(141)
(142)
(143)
(144)
(145)
(146)
(147)
(148)
(149)
(150)
(151)
(152)
(153)
(154)
(155)
(156)
(157)
(158)
(159)
(160) .
(161) .
(162)
(163)
(164)
(165)
(166)
(167)
(168)
(169)
(170)
(171)
(172)
(173)
(174)
(175)
(176)
(177)
(178)
(179)
(180)
(181)
(182)
(183)
(184)
(185)
(186)
(187)
(188)
(189)
(190)
(191)
(192)
(193)
(194)
(195)
(196)
(197)
(198)
(199)
(200)
(201)
(202)
(203)
(204)
(205)
(206) .
(207) "
(208) -$"
(209) %. .
(210) . , % & / 0 1 2 3 4 ,+ ,, ,,. ,/ ,0 $) $* $+ $, %# , % & / 0 1 2 3 4 ,+ ,, ,,. ,/ ,0 $) $* $+ $, %#. . %
(211) $ % ' ( ) * + , $# $$. . . ' ! ( ' "! ( "' ! ( "' "! ( " "' ! ( "' "! ( " ' ! ( ' "! ( . . ' ! ( ' "! ( "' ! ( "' "! ( " "' ! ( "' "! ( " ' ! ( ' "! (
(212) %& # %
(213) & # .
(214) Supplementary Table 3: Endocytic Profiles (compounds with parameters in bold are endocytic hits). Ag1478_10 Dmso 0.1% Miltefosine IBS-100 IBS-101 IBS-102 IBS-103 IBS-104 IBS-105 IBS-106 IBS-107 IBS-11 IBS-15 IBS-16 IBS-17 IBS-2 IBS-20 IBS-21 IBS-23 IBS-24 IBS-30 IBS-33 IBS-34 IBS-35 IBS-36 IBS-41 IBS-42 IBS-43 IBS-44 IBS-45 IBS-46 IBS-47 IBS-49 IBS-50 IBS-56 IBS-58 IBS-63 IBS-64 IBS-65 IBS-66 IBS-68 IBS-79 IBS-83 IBS-85 IBS-87 IBS-95. EGF number EGF mode EGF mode of vesicle Area (volume Area (mean masked weighted) int. weighted) 2.277 4.573 7.175 -0.194 -0.423 -0.733 0.357 -0.299 -0.129 0.188 -0.138 0.298 0.698 -0.671 -0.200 0.391 -0.509 0.142 0.993 -0.133 0.129 0.830 0.110 0.426 0.548 0.436 1.068 0.660 0.439 1.020 0.225 -1.359 -0.440 -0.585 0.579 0.048 -1.239 0.140 -0.017 0.260 -0.478 -0.809 -1.360 2.574 3.290 0.013 -0.616 -0.225 0.567 -0.373 -0.135 -0.166 0.077 -0.077 0.613 -0.902 -0.509 0.251 -0.198 -0.905 0.121 0.439 -0.041 -0.517 -0.303 1.351 -1.141 -0.220 -0.847 -1.557 -0.198 0.246 -0.282 -0.434 -0.555 0.113 -0.227 -0.210 -1.329 0.626 0.384 -1.043 0.644 -0.065 -0.393 0.196 -0.229 -0.254 -0.706 -1.079 0.321 -0.955 -1.227 -1.753 0.956 2.230 -0.962 0.988 0.344 -2.331 1.410 0.890 -1.886 1.938 2.859 -0.681 -0.352 0.576 -0.851 -0.120 0.443 -0.567 -0.054 0.752 -0.549 -0.435 -0.767 -0.226 0.243 0.380 1.924 -0.488 0.215 -0.337 -0.346 -0.012 -0.967 0.505 0.891 0.439 -0.476 0.324 2.565 -1.718 -1.064 1.338 -1.367 -0.671. . EGF mode Area 2.762 -0.509 -0.271 -0.037 -0.481 0.045 0.356 0.042 0.631 0.375 -0.473 0.000 0.040 -0.450 2.408 -0.153 -0.284 -0.107 -0.633 -0.388 0.025 0.399 -0.497 0.124 -0.429 -0.132 0.352 0.291 -0.536 -0.608 -0.942 0.949 0.609 0.895 1.570 0.110 0.298 -0.031 -0.228 0.127 -0.389 -0.311 0.333 -0.078 -1.086 -0.961.
(215) !. EGF mode Mean Intensity (volume weighted) -2.824 0.000 2.112 0.254 1.561 1.380 0.612 1.902 0.088 0.762 1.318 -0.296 -0.780 -0.177 -1.227 0.503 -0.102 0.070 0.396 -0.241 -0.224 0.866 -1.090 -0.434 -0.766 -0.343 -0.744 -0.767 -1.072 0.438 1.185 -1.324 -1.226 -1.385 -1.471 -0.205 -0.717 0.151 -0.029 0.199 2.229 1.712 -1.214 0.330 2.077 1.864. EGF mode Mean Intensity (mean int. weighted) -2.556 0.059 1.286 0.368 1.262 1.319 0.676 1.550 0.300 0.794 1.237 -0.257 -0.728 0.046 -1.015 0.720 0.289 0.154 0.383 -0.131 -0.058 0.883 -0.770 -0.256 -0.771 0.084 -0.621 -0.550 -0.932 0.597 1.655 -1.082 -1.023 -1.083 -1.319 0.045 -0.655 0.550 0.073 0.222 1.616 1.619 -0.810 0.670 1.947 1.660.
(216) IBS-96 IBS-97 IBS-98 J405S J419E J428E J429E J430E J431Y J437Y J438Y J439S J443E J444C J447C J448E J449E J453E J454E J455Y J459Y J460Y J461Y J462E J469Y J473C J475E J476S J477C J478C J479Y J480Y J482C J483C J484C J485E J486Y J488Y J489Y J491C J494E J495Y J497Y J500E J501E J502E J503E J505Y J506Y J507Y J508Y J509Y J510Y. 0.662 1.010 0.648 0.747 -0.818 0.707 0.115 0.436 0.169 1.017 1.181 0.467 -0.078 1.433 0.027 0.934 0.896 0.854 0.668 1.905 0.466 -0.204 -1.146 -0.266 -0.138 -1.246 0.679 -0.294 0.022 -0.326 -0.522 0.746 -0.357 -0.001 0.286 0.748 -0.010 0.267 -0.021 -1.269 0.019 -0.558 -0.706 -0.223 -0.294 -0.624 -0.543 0.345 -0.054 -0.825 -1.280 -0.496 -1.524. -0.128 -1.353 -0.176 0.346 2.881 -0.234 0.673 0.701 -0.182 -0.567 1.366 0.074 2.263 -1.194 1.113 -0.743 0.362 1.289 0.471 -1.242 -0.101 0.131 4.815 -0.155 0.708 2.305 -0.933 -0.252 -0.071 0.072 3.255 -0.437 0.552 0.351 -0.548 -1.577 0.078 -0.560 -0.220 0.387 -0.904 0.611 0.734 0.622 -0.046 -0.167 -0.992 2.125 0.534 2.394 1.785 1.917 2.460. . -0.304 -0.686 0.716 0.609 1.868 -0.002 0.601 0.930 -0.075 -0.325 1.902 0.462 2.391 -0.706 1.335 -0.430 0.932 1.104 0.950 -0.724 -0.145 -0.254 4.659 -0.758 0.206 1.439 -0.733 -0.098 -0.347 -0.049 3.194 -0.368 0.012 -0.044 -0.816 -1.487 -0.108 -0.170 -0.090 0.180 -1.399 0.144 -0.046 -0.216 -0.214 0.195 -0.546 1.171 0.538 1.244 0.963 0.875 1.302.
(217) !. -0.559 -0.872 0.003 0.681 2.352 0.216 0.942 1.192 0.136 -0.779 1.023 0.330 2.734 -0.854 1.532 -0.593 1.172 1.068 0.944 -1.189 0.281 0.113 5.393 -0.524 0.254 2.221 -1.089 0.173 -0.342 0.254 2.986 -0.514 0.809 0.306 -0.880 -1.790 -0.047 -0.675 -0.059 0.926 -1.367 0.799 0.357 0.000 -0.103 0.273 -0.574 0.819 0.293 1.645 1.874 1.147 2.016. 1.358 2.296 0.636 0.736 -1.270 1.492 -0.834 0.186 0.584 1.603 -0.231 0.179 -0.242 1.294 0.646 0.407 1.194 -0.889 0.346 4.199 0.015 -0.787 -1.443 -0.646 -1.063 -1.162 0.845 -0.429 0.347 0.228 -1.103 0.380 -0.802 -0.542 0.016 1.509 0.241 0.923 -0.142 -0.326 0.304 -1.098 -1.033 -0.504 -0.281 0.141 0.831 -0.723 0.071 -1.174 -1.494 -0.910 -1.238. 1.265 1.827 0.785 0.585 -1.228 2.444 -0.425 0.573 0.695 2.084 -0.674 0.135 -0.195 1.612 0.847 0.393 1.669 -0.922 1.679 3.674 0.032 -0.602 -1.269 0.160 -0.928 -0.945 0.884 0.091 0.537 0.175 -1.123 0.515 -0.407 -0.390 0.042 2.050 0.323 0.530 -0.048 -0.398 0.318 -0.895 -0.894 -0.574 -0.239 0.230 1.280 -0.863 0.006 -1.208 -1.364 -1.018 -1.158.
(218) J511Y J513Y J514E J515Y J518Y J519Y J520Y J521Y J522E J523E J524Y J525E J528E J529E J530Y J531Y J532Y J533Y J534E J536Y J537E J538E J539C J540C J541E J542E J543Y J545Y J546Y J547E J548Y J549E J550E J551E J552E J553E J554Y J555Y J556Y J557E J558E J559S J560S J561Y J562Y J563Y J564Y J565Y J566E J567E J568E J569E J570E. -1.156 -1.354 -0.593 0.069 0.841 1.256 0.993 0.343 0.718 0.863 0.513 0.796 -0.736 0.291 0.878 0.213 -0.356 0.926 1.745 0.771 -0.618 -0.216 -0.494 -0.751 -0.986 0.182 -0.049 -0.647 -0.193 -0.372 -0.018 0.884 0.455 0.317 0.132 -0.550 -0.018 0.698 -0.279 -0.179 -0.550 -1.083 -0.595 -1.214 -0.466 -0.310 -1.217 -0.154 -1.323 -0.908 -0.645 -2.352 -1.185. 1.287 0.535 0.048 0.354 -0.283 -0.292 -0.375 2.406 0.446 -1.142 0.002 3.702 4.111 1.158 -0.340 0.791 2.008 -0.460 -0.494 -1.334 1.565 0.968 3.893 0.625 4.557 0.517 3.438 2.085 1.478 -0.392 -0.725 0.065 -0.410 1.110 0.229 0.718 -0.779 -0.424 1.349 0.397 -0.146 1.401 3.051 0.838 0.119 -0.552 0.544 -1.174 3.591 0.796 -0.655 6.377 2.253. . 1.187 0.665 -0.104 0.827 0.198 0.272 0.008 0.947 -0.261 -1.097 -0.360 3.813 4.129 1.922 -0.074 0.370 1.872 -0.239 -0.328 -0.588 0.940 0.519 3.157 0.400 3.132 0.203 1.992 1.701 0.663 -0.515 -0.613 0.165 -0.632 0.401 0.133 0.375 -0.851 -0.839 1.101 -0.050 -1.081 0.968 2.843 -0.435 -0.024 -0.443 0.448 -0.880 2.969 -0.423 -0.796 6.525 1.915.
(219) !. 1.250 0.911 -0.217 1.338 0.028 0.234 -0.387 1.200 0.075 -1.155 -0.196 2.757 4.919 2.443 -0.086 0.764 1.896 -0.484 -0.707 -0.575 1.378 0.740 3.313 0.351 3.380 0.626 2.128 2.445 1.508 -0.472 -0.931 -0.098 -0.923 0.353 0.523 0.635 -0.767 -0.810 1.382 0.206 -0.937 1.578 3.709 0.508 0.256 -0.515 0.839 -0.991 3.006 -0.010 -0.571 6.243 2.304. -0.874 -0.602 0.776 -0.688 0.621 1.589 1.267 -1.263 -0.707 1.582 -1.000 -1.240 -1.103 -0.539 0.918 -0.756 -1.084 -0.097 0.618 2.106 -0.723 -0.345 -1.195 -0.538 -1.355 -0.131 -1.185 -1.137 -1.066 -0.336 0.907 0.115 -0.134 -0.814 -0.517 -0.522 -0.402 -0.081 -0.788 -0.049 -0.569 -1.176 -1.233 -0.727 -0.135 0.240 -0.471 0.936 -1.473 -1.180 -0.602 -1.722 -0.649. -0.934 -0.390 0.246 -0.215 0.688 1.772 1.022 -1.309 -0.740 1.985 -0.943 -1.458 -1.131 -0.022 0.810 -0.583 -1.027 0.051 0.375 2.387 -0.752 -0.530 -1.299 -0.206 -1.329 0.080 -1.222 -1.133 -0.954 0.079 0.692 0.219 -0.069 -0.926 -0.481 -0.497 -0.256 -0.169 -0.459 -0.166 -0.565 -1.028 -1.173 -0.630 -0.044 0.301 -0.472 0.872 -1.428 -1.080 -0.494 -1.709 -0.608.
(220) J571E J572E J573Y J575Y J576E J577E J578Y J580Y J582C J583E J584E J585Y J586E J588Y J589E J590Y J591E J592C J593C J594E J595E J596C J597E J598E J599E J600E J601Y J602E J603E J604E J605Y J606E J607Y J608E J609E J610Y J611Y J612Y J613C J614Y J615E J616E J617E J619Y J620Y J621E J622Y J623E J624E J625E J626Y J627E J628E. -1.605 -0.794 -0.300 -0.792 -0.465 -0.367 0.275 0.374 0.753 -0.516 1.664 0.516 -0.477 -1.167 1.207 0.319 -0.628 -0.517 0.154 1.127 -0.365 0.743 0.100 1.228 0.180 -0.935 -0.530 0.907 -0.323 -1.010 -0.007 -0.164 0.363 -0.344 -0.322 -1.122 -0.637 0.519 -0.435 -0.315 -0.647 -1.435 -0.533 -1.327 -1.597 -0.297 -0.604 0.316 -0.409 -0.282 -0.029 -1.038 -1.334. 2.519 2.023 -0.174 0.150 2.140 2.569 -0.828 0.543 -1.405 2.681 -1.129 -0.090 1.546 2.823 0.300 0.979 1.229 2.322 0.297 -0.092 2.744 -1.026 0.017 -0.845 -0.293 1.180 0.742 -1.915 0.836 0.900 -1.140 0.474 -0.767 -0.258 0.176 1.690 -0.091 -0.800 1.675 1.046 -0.471 1.100 -0.728 0.358 2.313 -1.493 0.697 -1.025 0.466 0.673 0.429 3.007 1.959. . 1.952 1.588 -0.754 -0.507 1.540 2.491 -0.498 0.864 -1.141 2.535 -0.644 -0.087 1.910 2.780 0.368 0.594 0.461 1.985 -0.028 -0.109 2.325 -0.631 -0.884 -1.170 -0.258 0.304 -0.341 -1.310 0.073 0.257 -0.768 0.071 -0.918 -1.116 -0.140 0.015 -0.611 -1.290 1.072 0.744 -0.259 0.287 -0.774 -0.555 1.551 -1.496 -0.428 -1.031 0.530 0.620 -0.316 2.104 0.570.
(221) !. 2.302 1.664 -0.947 -0.226 1.901 2.063 -0.956 1.448 -1.563 2.627 -0.767 -0.149 2.242 3.221 -0.089 0.635 1.121 2.499 0.472 -0.496 2.323 -0.872 -0.415 -1.493 -0.043 0.665 -0.201 -2.083 0.499 0.374 -0.868 0.066 -1.024 -0.975 0.051 0.713 -0.532 -1.203 1.285 1.035 -0.459 0.974 -0.662 -0.156 2.148 -1.695 -0.471 -0.927 0.861 0.803 -0.294 2.879 1.300. -1.316 -0.474 -0.241 -0.793 -1.166 -0.582 1.688 -0.843 0.373 -0.869 2.773 0.180 -0.999 -1.548 1.098 -0.877 -0.618 -0.756 0.743 0.040 -0.859 5.502 -0.802 -0.007 -0.368 -1.276 -1.251 6.469 -0.949 -0.295 1.610 -0.616 0.799 -0.867 -0.287 -0.968 -0.143 -0.369 -0.450 -0.953 -0.130 -0.976 0.588 -0.976 -1.288 0.462 -0.946 2.886 0.437 -0.885 -0.798 -1.174 -0.978. -1.170 -0.598 -0.348 -0.732 -1.117 -0.768 1.395 -0.555 0.537 -0.190 2.711 0.012 -0.881 -1.504 0.541 -0.949 -0.384 -0.410 0.977 -0.114 -0.982 4.702 -0.671 -0.130 -0.154 -1.122 -1.227 5.569 -0.829 -0.517 1.995 -0.692 0.859 -0.720 0.269 -0.950 -0.287 -0.035 -0.314 -0.811 -0.088 -0.919 0.612 -0.954 -0.669 0.847 -1.018 2.608 0.569 -0.950 -0.864 -1.166 -1.021.
(222) J629E J631Y J632E J633E J634E J635E J636Y J637Y J639E J640E J641E J643Y J644Y J645E J646E J647E J648E J649Y J650Y J651E J652E J653Y J654Y J655Y J656Y J657E J658E J659Y J660Y J661Y J662C J663Y J664Y J665Y J666Y J667Y J668Y J669Y J670Y J671Y J672Y J673Y J674Y J675Y J676Y J677Y J678Y J679Y J680Y J681Y J682Y J683Y J684Y. -0.487 -0.809 -0.319 -1.146 -1.115 -0.113 0.592 -0.197 -0.085 1.969 0.835 0.206 1.008 2.771 0.403 1.192 0.483 0.101 0.807 0.073 1.201 0.250 -0.086 -0.055 0.364 -0.459 1.633 0.149 0.138 0.267 0.284 -0.100 -0.238 0.409 0.250 0.087 0.772 -1.003 0.061 0.333 -0.680 0.076 -0.483 -1.113 0.156 -0.696 -0.856 0.669 0.454 0.013 0.243 0.959 -1.246. 1.039 1.685 0.958 1.197 1.971 0.266 -0.488 2.255 -0.008 -1.542 -0.507 -0.390 -0.734 -0.378 -0.319 -0.020 0.269 0.663 -0.992 0.143 -1.822 -0.049 -1.082 0.820 0.766 0.595 -1.348 -0.988 1.534 0.359 -0.989 0.750 -0.543 -0.266 -0.955 0.657 -0.424 0.352 0.754 1.092 1.336 0.228 -0.726 -0.540 -0.081 0.575 -0.286 -1.305 -0.697 -1.242 -0.074 -0.870 1.095. . 0.401 1.113 0.401 0.580 1.501 0.347 -0.216 0.290 0.218 -0.782 -0.766 -0.051 -0.221 0.393 -0.455 0.463 0.720 -0.018 -0.612 0.053 -1.028 -0.177 -0.686 0.091 0.267 0.196 -1.234 -1.186 0.895 0.646 -0.582 0.204 -0.694 -0.317 -1.280 0.355 -0.807 -0.249 0.231 0.156 1.429 0.440 -0.946 -0.970 -1.091 -0.195 -0.619 -1.005 -1.234 -1.086 -0.817 -0.901 0.692.
(223) ! !. 0.412 1.458 1.060 1.277 1.660 0.909 0.043 0.652 0.010 -1.312 -0.569 0.044 -0.469 -0.297 -0.522 0.322 0.989 0.343 -0.652 0.167 -1.614 -0.354 -0.990 0.022 0.417 0.605 -1.683 -1.263 0.983 0.646 -0.638 0.630 -1.034 -0.670 -1.328 0.153 -0.969 0.364 0.310 0.162 1.820 0.206 -0.960 -0.961 -1.003 0.208 -0.746 -1.503 -1.215 -1.188 -0.706 -1.259 1.165. -0.772 -1.264 -0.721 -0.590 -1.060 -0.360 0.744 -1.299 0.695 4.633 0.053 0.680 2.842 1.185 1.734 1.623 -0.130 -1.097 0.744 -0.120 3.637 1.409 2.314 -0.964 -0.390 -1.114 3.852 0.001 -1.058 1.079 1.196 0.028 0.272 1.075 0.071 -0.598 -0.224 -1.274 -0.509 -0.931 -0.711 1.084 -0.128 -0.103 -0.738 -1.090 -0.509 1.226 -0.356 0.351 -0.786 0.374 -1.359. -0.738 -1.173 -0.630 -0.544 -1.010 -0.070 1.009 -1.331 1.012 4.342 0.478 1.522 3.141 0.663 1.926 1.923 0.337 -1.115 1.127 0.006 3.375 0.930 2.051 -0.984 -0.531 -0.827 3.068 0.449 -1.110 0.859 1.777 0.046 -0.030 1.018 0.082 -0.755 -0.217 -1.178 -0.415 -1.028 -0.656 0.641 -0.151 -0.255 -0.719 -0.982 -0.656 1.027 0.213 0.052 -0.741 0.108 -1.232.
(224) J685Y J686Y J687Y J688Y J689Y J690C J692Y J693Y J694Y J696Y J697Y J698Y J699Y J700E J702Y J703Y J704Y J705Y J706Y J707Y J708Y J709Y J710Y J711Y J712Y J713Y J714Y J715Y J718Y J719Y J720Y J775Y KA-10 KA-12 KA-13 KA-14 KA-17 KA-2 KA-22 KA-23 KA-24 KA-25 KA-26 KA-27 KA-28 KA-29 KA-3 KA-30 KA-31 KA-32 KA-33 KA-34 KA-36. -0.663 -1.877 -0.966 0.257 -1.262 -0.460 0.726 1.684 0.230 0.842 1.844 0.931 1.349 0.582 1.397 -1.314 1.042 -0.904 0.106 0.946 -0.741 0.862 0.318 -0.443 0.923 0.709 1.700 -0.184 0.608 0.746 1.435 0.339 0.393 0.330 0.228 -0.729 -0.932 -0.117 0.032 1.162 -0.132 -0.293 -0.044 -0.308 1.129 -0.456 0.130 -0.957 -1.634 -1.273 -0.912 -0.310 -0.555. 0.316 2.923 0.661 -0.592 1.361 -0.843 -0.032 -0.255 -0.884 -0.598 -1.454 -0.722 -1.316 -0.898 0.291 0.341 -0.073 0.480 -0.564 -0.919 -0.908 -0.551 -0.060 -0.104 -0.654 -0.520 -0.695 1.340 -1.150 -0.172 -0.824 -0.613 -1.075 -0.186 0.298 -0.754 0.165 -0.048 0.422 -0.545 -0.243 -0.436 -0.250 -0.324 -0.538 0.016 0.229 -0.674 0.532 0.200 -0.494 0.755 -0.272. . 0.071 2.655 0.079 -0.921 1.008 -1.241 0.051 -0.322 -0.260 0.757 -0.662 -0.156 -1.067 -0.317 0.837 1.082 0.019 0.951 0.332 -0.870 -0.201 -0.409 -0.100 -0.475 -0.445 -0.545 -0.785 1.990 -1.150 -0.678 -0.507 0.015 -0.680 -0.644 0.586 -0.903 -0.042 0.065 0.230 0.102 -0.938 -0.435 0.115 -0.756 -0.140 0.066 0.391 -0.601 0.819 -0.464 -0.868 1.336 -0.251.
(225) " !. 0.467 3.447 0.271 -1.154 1.530 -1.058 -0.303 -0.372 -0.653 0.213 -0.773 -0.360 -1.016 -0.474 0.095 0.305 -0.292 0.525 -0.027 -0.768 -0.298 -0.448 0.257 -0.331 -0.870 -0.758 -0.500 0.972 -0.970 -0.261 -0.459 -0.234 -0.636 -0.422 0.005 -0.526 -0.294 0.181 0.388 0.792 -0.386 -0.065 -0.061 -0.378 -0.468 -0.012 0.257 -0.453 0.333 -0.122 -0.206 0.789 -0.682. -1.204 -1.104 -0.976 0.291 -0.617 0.075 0.482 0.221 -0.151 0.728 1.545 1.289 0.841 2.726 -1.211 -0.596 2.672 -0.181 0.820 1.430 1.591 1.080 1.239 1.089 0.362 2.553 -0.278 -0.486 0.883 -0.111 1.104 2.920 1.318 1.195 -0.495 0.070 -0.529 0.643 -0.637 5.331 0.327 0.906 0.438 2.115 1.894 -0.208 -0.189 -0.061 -0.807 -0.619 -0.692 -0.529 0.262. -0.919 -0.854 -0.724 -0.098 -0.225 0.047 0.623 0.265 -0.058 1.136 1.653 1.363 1.177 2.485 -0.852 -0.272 2.245 -0.183 0.750 1.222 1.536 1.215 1.329 1.131 0.586 1.937 -0.064 -0.281 0.827 0.138 0.857 1.786 1.757 1.438 -0.388 0.094 -0.549 0.720 -0.429 4.465 0.412 0.674 0.572 1.989 2.065 0.074 -0.032 -0.065 -0.649 -0.340 -0.336 -0.383 0.477.
(226) KA-37 KA-38 KA-4 KA-42 KA-5 KA-50 KA-52 KA-53 KA-6 KA-7 KA-9. -0.729 -0.591 0.239 -1.279 -1.382 -0.004 -1.619 -0.797 0.011 -0.857 -0.528. -0.175 0.101 -0.462 0.655 0.945 -0.169 -0.301 -0.306 -0.940 0.373 0.279. . 0.184 0.019 -0.797 0.725 1.355 -1.072 -0.422 -0.563 -0.949 0.297 0.536.
(227) # !. -0.049 -0.175 -0.429 0.538 0.558 -0.058 0.185 -0.351 -0.664 -0.055 0.931. -0.148 -0.392 0.686 -0.952 -0.511 0.012 -0.729 0.406 0.381 -0.599 -0.484. 0.004 -0.314 0.674 -0.620 -0.270 0.138 -0.566 0.298 0.561 -0.436 -0.364.
(228) ). EGF mode Mean Intensity -1.692 0.258 1.211 0.160 0.658 0.862 0.243 0.573 -0.131 0.320 1.062 -0.617 -0.696 -0.010 -1.216 0.458 0.250 -0.090 0.374 -0.182 0.075 0.742 -0.680 -0.082 -0.295 -0.101 -0.611 -0.748 -0.774 0.273 1.935 -0.745 -1.054 -0.465 -1.124 0.118 -0.256 0.298 0.206 0.062 1.546 1.121 -0.622 -0.164 1.070 1.146. EGF mode Integral intensity (volume weighted) -0.761 -0.383 2.368 0.121 1.553 2.134 0.569 1.167 0.701 1.186 1.108 -0.183 -0.706 0.468 -0.767 0.468 0.054 -0.110 0.866 -0.273 -0.160 1.382 -0.594 -0.535 -0.770 0.051 -0.632 -0.849 -0.518 0.240 1.645 -1.026 -0.865 -0.924 -0.949 -0.226 -1.016 0.191 0.056 0.034 2.117 1.777 -0.670 0.584 2.111 1.837. EGF mode Integral intensity (mean int. weighted) -1.318 -0.322 1.323 0.250 2.093 2.626 1.426 1.393 1.171 1.399 1.159 0.098 -0.763 0.544 -0.881 0.654 0.099 0.075 1.124 -0.314 -0.007 2.165 -0.486 -0.433 -0.674 0.087 -0.683 -0.912 -0.522 0.588 2.280 -0.894 -0.919 -0.731 -0.700 -0.255 -0.907 0.432 0.342 0.126 3.001 2.288 -0.623 0.907 3.282 2.335. . EGF mode Integral intensity -1.889 -0.148 2.214 0.684 0.771 1.843 1.082 1.223 0.388 1.029 0.988 -0.918 -0.582 -0.810 -0.300 0.973 0.185 0.045 0.211 -0.216 0.175 1.427 -1.351 0.078 -0.788 -0.903 -0.632 -0.652 -1.948 0.315 1.778 -0.590 -1.127 -0.095 -0.818 0.269 -0.291 0.338 0.280 0.278 1.751 1.864 -0.877 -0.061 0.218 1.014. EGF Dist. to nuc. (volume weigted | nuc. size normalized) 5.419 -0.674 -0.518 -0.019 0.747 -0.295 2.079 0.328 0.236 0.430 0.367 0.899 -0.096 -0.249 1.942 -0.635 1.152 1.941 0.770 -0.267 -0.437 -0.490 2.685 1.981 1.857 1.876 1.957 0.752 2.077 2.407 2.302 2.467 2.773 -0.063 0.640 1.614 1.030 1.580 1.401 -0.020 -0.701 -2.115 -0.064 -1.294 0.482 0.939.
(229) $ !. EGF Dist. to nuc. (mean EGF Dist. to int. weighted nuc. (nuc. | nuc. size size normalized) normalized) 4.157 3.441 -0.657 -0.408 -0.310 0.058 0.177 1.258 0.804 1.213 -0.182 0.928 1.790 1.993 0.277 1.367 0.234 0.903 0.559 1.814 -0.166 1.103 1.101 0.891 0.175 -0.223 0.187 0.429 1.296 0.988 -0.320 0.171 0.918 1.797 1.818 2.000 0.961 1.562 -0.018 0.057 -0.072 0.393 -0.318 -0.489 2.995 3.308 2.620 1.272 1.991 3.279 1.919 2.546 1.994 2.223 1.048 0.618 1.910 2.700 2.556 3.846 2.106 3.536 1.908 1.122 1.935 0.951 -0.110 -0.906 0.603 0.393 1.356 1.408 0.764 0.885 1.335 1.626 1.612 2.034 -0.056 0.027 -0.319 0.440 -1.541 -0.927 -0.108 -0.694 -1.198 -0.058 0.718 2.502 0.793 1.764.
(230) 1.045 1.801 0.076 0.492 -1.623 0.991 -0.806 -0.091 0.558 1.221 -0.429 0.144 -0.391 1.572 0.272 0.412 0.740 -1.015 0.846 3.375 0.092 -0.693 -2.116 -0.463 -1.114 -1.476 0.926 -0.200 0.108 0.670 -1.423 0.445 -0.917 -0.597 -0.092 1.481 0.207 0.875 0.170 -0.668 0.907 -1.080 -1.024 -0.345 -0.372 -0.242 0.825 -0.722 0.340 -1.347 -1.840 -1.268 -1.690. 1.408 1.974 1.954 1.939 -0.525 3.006 0.383 1.226 1.520 2.487 0.295 1.161 0.416 1.991 2.575 0.813 2.661 -0.893 1.992 5.895 0.325 0.096 -0.395 0.324 -0.654 -0.384 1.677 1.122 1.570 0.807 -0.495 0.894 0.176 0.278 0.689 2.620 0.585 1.426 0.446 -0.236 0.101 -0.649 -0.610 -0.573 0.126 0.165 1.769 -0.787 0.524 -1.370 -1.415 -1.136 -1.064. 1.589 2.620 1.964 1.477 -0.632 3.102 0.355 1.004 1.365 2.116 -0.675 0.559 0.359 1.952 1.616 0.311 2.681 -0.849 1.816 4.075 0.505 -0.253 -0.373 0.235 -0.604 -0.448 1.058 1.037 0.814 0.519 -0.708 0.589 0.181 -0.064 0.275 1.938 0.478 0.664 0.111 -0.254 -0.055 -0.545 -0.563 -0.760 0.006 0.027 1.383 -1.188 0.167 -1.499 -1.283 -1.206 -0.950. . 1.417 1.811 -0.055 1.887 -0.146 2.603 0.802 1.666 1.594 1.959 -0.559 0.806 1.283 1.946 2.296 0.569 3.226 -1.000 2.376 3.495 0.754 0.060 0.479 0.466 -0.402 0.055 1.221 1.227 0.835 1.207 0.112 0.515 0.569 0.127 0.385 1.459 0.850 0.945 0.462 0.334 0.387 -0.242 -0.579 -0.424 0.182 0.255 1.111 -1.189 0.846 -1.406 -1.511 -1.349 -0.899.
(231) % !. -0.274 -0.121 -0.668 -1.018 -0.480 0.042 0.118 -0.702 0.863 -0.328 2.808 -0.124 -0.326 -0.210 -0.166 0.080 -0.520 0.453 0.389 0.586 0.207 0.459 -0.339 0.294 0.997 0.524 -0.720 0.190 -0.123 0.367 0.712 0.096 -0.230 1.107 0.293 0.638 1.198 0.678 1.448 0.246 1.644 0.388 0.568 2.365 0.310 1.214 1.652 3.316 0.618 2.336 0.197 2.364 0.560. -0.191 0.089 -0.700 -0.762 -0.476 -0.030 0.056 -0.663 0.731 0.093 2.845 0.273 -0.344 -0.047 -0.075 -0.015 -0.441 0.771 0.399 0.794 0.502 0.520 -0.526 0.293 0.974 0.675 -0.405 0.180 0.084 0.581 0.444 0.263 -0.024 1.038 0.560 0.785 1.402 0.953 1.364 0.395 1.538 0.152 0.537 2.147 0.252 1.272 1.750 3.027 0.476 2.147 0.286 2.852 0.855. 0.210 0.849 0.119 -0.859 -0.715 -0.445 -0.296 -0.658 0.713 0.400 1.737 -0.068 -0.605 -0.107 -0.565 0.039 -0.601 0.375 -0.117 0.971 0.336 0.267 -0.891 0.030 0.828 0.073 -0.258 -0.050 0.039 0.201 0.127 0.037 -0.489 0.561 0.545 0.800 1.348 0.883 1.252 0.261 1.183 -0.468 0.274 1.417 0.463 0.956 1.418 1.908 0.113 1.292 -0.210 2.176 0.463.
(232) -0.923 -0.452 0.603 -0.341 0.615 1.414 1.069 -1.683 -0.675 1.681 -1.206 -2.050 -1.371 -0.294 0.521 -0.779 -1.453 0.057 1.258 1.755 -1.447 -0.365 -1.732 -0.599 -1.994 -0.402 -1.536 -1.468 -1.187 -0.212 0.666 0.265 -0.040 -0.923 -0.314 -0.705 -0.067 0.131 -0.856 0.137 -0.485 -1.207 -1.636 -1.117 -0.195 0.360 -0.795 0.407 -2.041 -1.243 -0.346 -2.651 -1.004. -1.103 -0.127 1.922 0.712 1.732 2.972 2.206 -1.321 -0.303 2.476 -1.071 -1.471 0.492 1.546 1.664 0.011 -0.967 0.836 0.790 3.065 -0.621 -0.063 -0.721 1.368 -0.200 0.807 -0.702 -0.530 -0.403 0.471 1.079 0.895 -0.221 -0.887 0.102 0.515 -0.397 -0.025 -0.451 0.463 -0.553 -0.808 -0.316 -0.534 0.051 0.177 -0.042 0.757 -1.423 -1.339 -0.457 -1.489 -0.360. -1.032 -0.081 0.087 0.776 1.271 2.221 1.162 -1.505 -0.482 2.213 -0.912 -1.569 -0.054 1.240 1.144 -0.078 -0.853 0.222 0.285 3.052 -0.508 -0.398 -0.927 0.343 -0.782 0.785 -1.120 -0.668 -0.581 0.233 0.641 0.483 -0.192 -1.063 0.061 0.097 -0.331 -0.321 -0.383 0.260 -0.580 -0.660 -0.496 -0.272 0.145 0.095 -0.217 0.642 -1.452 -1.134 -0.334 -1.511 -0.370. . -1.052 0.419 -0.013 2.021 1.853 2.759 1.748 -1.956 -0.596 2.420 -1.580 -2.029 1.601 2.500 1.244 0.207 -0.907 0.130 0.416 3.130 -0.526 0.113 -0.822 0.645 -0.167 1.027 -0.740 -0.346 -0.457 0.052 0.423 0.468 -0.516 -1.251 0.422 0.231 -0.392 -0.337 -0.161 0.292 -0.830 -0.113 0.535 -0.108 0.302 0.575 0.289 0.278 -1.429 -1.245 -0.399 -0.864 0.367.
(233) !. 2.613 1.248 2.690 -0.463 0.281 0.251 0.032 1.192 -0.237 -0.287 0.986 2.216 -0.823 -0.514 0.543 -0.146 0.078 0.014 1.215 0.548 0.937 0.658 0.733 -0.635 -0.532 -0.521 1.217 0.356 0.535 1.156 0.422 0.719 1.052 1.905 1.118 0.382 1.378 0.818 0.548 1.229 1.337 0.128 -0.849 0.712 0.821 1.511 -0.188 1.089 1.401 0.378 0.479 0.844 -0.176. 2.459 1.695 2.631 -0.417 0.393 0.579 0.423 1.555 0.059 0.105 1.048 2.241 -0.767 -0.594 0.759 0.126 0.262 0.263 1.268 0.631 1.007 0.901 1.035 -0.618 -0.510 -0.374 1.517 0.322 0.763 1.352 0.778 0.719 0.986 1.937 0.775 0.710 1.607 0.961 0.662 1.185 1.308 0.260 -1.088 0.746 0.655 1.630 -0.092 1.011 1.187 0.367 0.580 0.847 -0.354. 1.993 1.233 2.562 -0.955 0.262 0.451 0.598 0.754 -0.266 -0.299 0.753 1.932 -1.481 -1.083 0.291 -0.643 -0.070 0.646 1.073 0.234 0.535 0.593 0.433 -0.677 -0.844 -0.320 0.606 0.115 0.499 1.153 0.613 0.249 1.195 1.525 0.688 0.290 1.473 0.970 0.274 0.654 1.072 -0.079 -1.334 0.260 0.695 0.993 -0.213 0.680 0.685 0.067 0.312 0.291 -0.643.
(234) -1.674 -0.748 0.001 -0.752 -1.379 -0.844 0.931 -0.783 0.821 -0.994 2.807 -0.039 -1.144 -2.358 0.793 -1.126 -0.845 -1.148 0.290 0.129 -0.934 3.185 -0.545 0.425 -0.039 -1.485 -1.489 4.355 -1.009 -0.973 1.497 -0.505 1.037 -0.754 -0.218 -1.425 -0.473 0.025 -0.469 -1.028 0.154 -0.973 0.718 -1.146 -1.564 1.011 -1.046 1.439 -0.502 -0.978 -0.814 -1.493 -1.312. -1.197 -0.204 -0.358 -1.037 -0.429 0.632 1.897 0.374 1.522 0.713 3.641 0.381 -0.355 -1.351 2.035 -0.639 0.374 0.646 1.232 0.386 -0.010 5.775 -0.366 -0.203 0.284 -1.063 -1.235 6.783 -0.201 0.314 1.828 -0.396 1.478 -0.853 0.430 -1.117 0.060 0.060 0.039 -0.319 0.116 -1.168 0.369 -1.360 -0.574 0.524 -1.382 2.452 0.332 -1.108 -0.859 -0.966 -1.063. -1.085 -0.392 -0.545 -0.775 -0.490 -0.153 1.657 0.577 0.948 0.597 3.341 0.313 -0.303 -1.162 0.828 -0.817 0.390 0.460 0.942 0.081 -0.666 5.310 -0.484 -0.223 0.231 -0.991 -1.254 4.087 -0.291 -0.537 1.588 -0.557 0.717 -0.727 0.250 -1.106 -0.168 0.018 -0.257 -0.577 0.121 -1.018 0.547 -1.043 -0.437 0.988 -1.321 2.111 0.432 -1.019 -0.935 -1.032 -0.926. . -0.814 0.178 -0.551 -0.982 0.026 0.047 1.480 1.025 0.696 1.011 2.981 0.416 0.375 -1.545 1.080 -1.184 1.006 0.658 1.315 0.090 0.176 4.043 -0.369 -0.569 0.650 -1.053 -1.626 4.178 0.014 -0.768 1.714 -0.545 0.990 -0.957 0.295 -1.202 -0.178 0.002 0.227 -0.385 0.546 -0.779 0.766 -1.126 -0.101 0.444 -1.685 1.967 0.118 -1.131 -1.249 -0.571 -0.912.
(235) !. -0.251 0.570 0.675 1.156 -0.146 0.721 -1.457 -0.207 -0.613 -0.710 0.591 0.430 0.494 0.305 0.242 0.469 -0.806 0.143 0.021 0.748 0.492 0.327 0.433 0.001 -0.477 -0.420 -0.081 -0.831 -0.807 0.469 -0.645 0.638 0.565 -0.305 0.034 1.159 0.134 0.469 -0.335 0.290 -0.415 1.221 1.012 0.964 -0.257 1.657 1.658 0.067 0.123 1.775 1.513 0.489 -0.268. -0.013 0.783 0.903 1.196 0.203 0.884 -0.990 -0.267 -0.049 -0.423 0.603 0.684 0.557 0.689 0.314 0.681 -0.250 0.162 0.151 0.817 0.747 0.403 0.675 0.294 -0.504 -0.080 0.535 -0.393 -0.586 0.610 -0.491 0.842 0.526 -0.016 0.306 1.156 0.294 0.297 0.027 0.383 -0.426 1.441 1.028 0.935 -0.146 1.569 1.656 0.145 0.160 1.474 1.398 0.353 0.227. -0.509 0.476 0.514 0.822 -0.169 0.416 -0.875 -0.217 -0.195 -0.701 0.386 0.489 0.297 0.021 0.384 0.396 -0.658 -0.071 0.134 0.916 0.258 0.103 0.135 0.303 -0.945 -0.375 0.255 -0.370 -0.855 0.463 -0.314 0.421 0.224 -0.163 -0.044 0.557 0.070 0.029 -0.170 0.117 -0.614 0.785 1.035 0.385 -0.491 1.637 1.184 0.220 0.073 0.835 1.109 -0.150 -0.294.
(236) -0.852 -1.560 -0.847 -0.654 -1.212 -0.273 0.883 -1.800 0.449 4.020 0.507 0.790 1.704 1.497 0.647 1.184 0.046 -1.333 0.752 -0.207 3.281 0.167 1.563 -1.088 -0.437 -1.123 3.623 0.301 -1.360 -0.035 1.547 -0.446 0.269 0.774 0.242 -0.774 -0.085 -1.562 -0.489 -1.042 -0.997 0.996 0.123 -0.024 -0.549 -1.239 -0.628 1.085 0.117 0.594 -0.525 0.403 -1.725. -0.761 -1.179 -0.438 -0.355 -0.737 0.787 2.134 -1.501 1.803 4.281 0.910 2.047 4.103 1.753 1.981 3.069 1.053 -1.078 1.609 0.687 4.262 2.231 2.290 -1.209 0.644 -0.601 4.756 0.387 -1.081 1.304 2.260 0.377 0.008 1.122 0.091 -0.711 -0.078 -1.292 -0.276 -0.840 0.280 1.598 0.111 -0.695 -1.163 -1.181 -0.989 1.490 0.084 0.232 -0.769 0.904 -1.669. -0.881 -1.045 -0.353 -0.312 -0.801 0.559 1.346 -1.510 1.463 4.390 1.001 1.729 3.824 1.133 1.872 2.451 0.990 -0.936 1.658 0.560 3.967 1.062 1.485 -1.188 -0.108 -0.341 2.970 0.453 -1.101 1.233 2.285 0.458 -0.468 0.873 -0.149 -0.850 -0.300 -1.081 -0.409 -1.050 0.203 0.719 -0.020 -0.456 -1.155 -1.141 -0.899 1.015 -0.036 -0.207 -0.910 0.165 -1.310. . -1.185 -0.996 0.082 0.361 -0.714 1.176 1.862 -1.970 1.456 3.927 1.361 1.706 3.252 1.061 1.026 2.097 1.695 -1.320 1.494 0.507 3.147 -0.018 1.516 -1.530 -0.223 -0.263 3.229 0.585 -1.198 0.691 2.114 0.467 -0.715 1.098 -0.540 -0.821 -0.475 -1.404 -0.222 -1.385 0.462 1.185 -0.075 -0.802 -1.630 -1.561 -1.460 0.896 -0.129 -0.408 -1.278 -0.166 -1.616.
(237) !. 0.626 0.581 -0.815 0.352 0.764 0.046 -0.704 0.924 -0.226 0.386 -0.292 0.322 0.209 1.879 -0.102 0.011 -0.358 0.493 0.562 0.838 0.541 1.110 0.960 1.106 0.618 0.174 -0.484 0.061 -0.051 0.510 1.489 -0.214 0.641 0.241 1.030 0.295 0.883 1.237 0.897 0.630 -0.068 1.234 0.973 1.608 3.032 1.638 2.186 1.117 1.695 1.697 1.539 0.715 0.726. 0.765 0.637 -0.578 0.184 0.807 0.164 -0.241 1.412 -0.103 0.479 -0.166 0.324 0.221 1.608 0.123 0.147 -0.349 0.581 0.696 1.046 0.802 1.256 1.343 1.307 0.902 0.377 -0.171 0.543 0.206 0.463 1.556 0.361 0.819 0.696 1.419 0.365 1.068 1.453 0.929 0.752 0.225 1.209 1.283 1.838 3.221 2.100 2.010 0.958 1.743 1.709 1.591 0.556 0.908. 0.602 0.282 -1.035 -0.210 0.279 -0.044 -0.587 0.518 -0.124 0.738 -0.508 0.613 0.491 1.875 0.104 0.195 -0.635 0.367 0.379 0.827 1.182 1.024 1.403 0.975 0.491 0.190 0.370 0.188 -0.109 0.481 1.237 0.083 0.729 0.258 1.014 0.223 0.734 0.978 0.598 0.492 -0.045 0.904 0.744 1.238 2.504 1.618 1.922 0.890 1.261 2.154 1.174 0.504 0.260.
(238) -1.135 -0.608 -1.216 -0.023 -0.671 0.179 0.386 0.419 -0.370 1.042 0.733 0.388 1.020 1.769 -0.804 -0.622 1.959 -0.491 0.450 0.710 0.956 0.755 0.879 0.749 0.649 1.907 0.266 -0.541 0.855 -0.006 0.912 1.268 1.312 1.222 -0.337 0.081 -0.527 0.161 -0.422 1.149 0.091 0.565 0.300 1.551 1.312 0.156 -0.183 0.422 -0.289 0.039 -0.483 -0.766 0.652. -0.913 -0.713 -0.978 0.087 0.028 -0.087 1.657 -0.147 1.328 0.836 1.459 1.692 0.525 2.495 -0.381 -0.220 3.655 -0.153 1.753 3.357 1.623 0.805 0.913 0.941 0.807 2.406 -0.164 -0.292 1.157 -0.145 0.794 3.072 1.412 1.292 -0.292 -0.199 -0.344 0.652 -0.458 5.138 0.065 1.051 0.640 2.826 2.888 0.326 -0.156 -0.569 -0.621 -0.523 -0.374 -0.278 0.641. -0.656 -0.639 -0.857 -0.290 0.046 -0.022 1.630 0.047 1.362 1.273 2.001 2.357 0.973 3.466 -0.303 -0.104 3.747 -0.173 1.437 4.550 2.206 0.990 1.448 1.441 0.586 2.513 0.013 -0.151 1.395 0.126 0.908 1.732 1.942 1.546 -0.259 -0.265 -0.244 1.300 -0.391 6.477 0.423 1.134 0.932 4.051 3.583 0.158 -0.197 -0.510 -0.707 -0.417 -0.205 -0.084 0.635. . -0.539 1.588 -1.437 -0.982 0.224 -0.117 0.188 0.070 -1.657 1.620 0.001 0.527 0.140 2.125 -1.966 -0.556 2.693 0.159 0.465 0.440 1.783 1.000 1.892 1.381 0.387 1.979 -0.303 0.167 0.571 -0.064 0.657 2.293 1.869 1.616 -0.351 0.133 -0.986 0.834 -0.432 3.806 0.459 0.926 0.425 2.369 1.709 0.040 0.098 -0.032 -0.364 -0.491 -0.882 -0.605 0.185.
(239) !. 0.832 -0.594 -0.588 1.100 1.413 0.663 -1.534 -0.268 2.196 0.457 -0.309 -0.352 1.204 -0.136 0.900 -0.419 -0.047 0.177 0.872 4.994 -0.205 0.775 -0.422 -0.101 -0.409 -0.226 0.705 1.119 -0.647 3.634 0.433 -0.935 -0.136 -0.934 -0.276 1.519 -1.405 0.128 0.770 2.459 0.222 -0.002 1.613 -1.476 -1.264 -0.895 0.312 2.635 0.973 5.973 0.793 1.380 1.236. 0.909 -0.505 -0.026 1.542 1.589 0.899 -1.011 -0.318 1.967 0.669 0.032 -0.491 1.114 -0.046 0.943 -0.613 0.132 0.202 0.508 4.898 -0.139 0.901 -0.344 0.079 -0.208 -0.120 0.679 1.017 -0.345 3.168 0.440 -0.579 -0.019 -0.395 -0.178 1.553 -1.209 0.304 0.641 2.043 0.193 -0.004 1.514 -1.165 -0.913 -0.558 0.577 2.817 0.735 5.250 0.725 1.014 1.190. 0.514 -1.084 -0.469 1.297 0.947 0.568 -0.501 0.766 2.334 0.665 1.423 0.578 2.589 1.557 1.998 -0.512 0.765 0.986 1.590 7.602 1.750 2.013 0.212 0.073 0.498 0.006 1.113 0.823 0.812 3.287 1.490 0.058 0.912 -0.195 -0.016 1.188 -0.751 0.681 0.606 1.796 0.142 1.080 1.759 -1.354 -0.358 -0.203 0.754 3.344 0.422 4.353 1.350 1.455 2.209.
(240) -0.112 0.002 0.200 -0.484 -0.421 0.120 -0.350 0.465 0.653 -0.754 -0.655. -0.126 -0.392 0.313 -0.709 -0.086 -0.278 -0.734 0.140 0.208 -0.452 -0.410. -0.056 -0.343 0.414 -0.925 0.254 0.111 -0.772 0.466 0.186 -0.235 -0.280. . 0.130 0.127 0.515 -0.404 -0.091 0.029 -0.426 0.248 -0.114 -0.671 -0.384.
(241) !. 0.281 -0.448 -0.320 1.818 -0.644 1.193 4.046 0.918 -0.850 -0.044 0.072. 0.340 0.031 -0.101 1.811 -0.431 1.017 2.530 0.957 -0.739 0.039 0.098. 1.305 0.735 0.515 1.854 -0.518 1.299 1.830 1.581 -0.450 0.369 1.428.
(242) TF Number TF mode TF mode of Vesicles Area (volume Area (mean (masked) weighted) int. weighted) 0.708 1.157 1.098 0.488 -1.365 -0.936 0.204 -1.259 -1.215 0.191 -0.164 0.319 0.894 1.523 0.874 -0.479 -0.137 1.563 1.363 -1.526 -0.389 -0.086 -1.612 -0.601 -0.410 -1.486 -0.365 -0.338 0.410 0.873 -0.125 -1.352 0.689 -0.993 -2.068 -1.899 -1.197 -0.512 0.769 -1.036 -1.634 -0.244 -1.679 -0.782 -1.205 -0.115 -2.328 -0.879 0.354 -2.808 -1.188 0.029 -1.253 -2.035 -1.004 -0.417 0.839 0.687 -2.540 -2.254 0.717 -0.839 -0.706 -1.009 1.688 3.476 -1.580 0.298 0.919 -1.514 4.451 4.458 -0.778 0.804 1.106 -0.990 -1.249 1.602 -1.745 -0.534 -0.588 -0.343 -0.534 -1.295 -4.425 1.264 -0.380 -0.230 -0.304 -0.313 -0.678 0.863 0.161 -2.132 0.870 2.585 -0.347 -0.348 -1.701 -1.637 0.580 0.979 -1.281 0.944 -0.111 -0.655 1.064 2.288 -0.284 -1.207 -2.983 -0.632 1.898 2.477 -0.488 0.717 1.560 0.211 -1.535 -0.219 0.266 0.235 0.803 -1.803 0.821 1.442 -2.753 -1.716 0.514 0.140 -1.913 0.272 1.144 -3.159 -1.310 1.019 -1.268 0.245. . TF mode Area 0.812 -1.132 -1.469 0.371 0.930 0.447 -0.841 -0.325 -0.628 1.031 -0.012 -0.733 0.849 -0.366 0.427 -0.960 -1.302 -1.457 0.170 -1.551 -0.709 2.492 1.458 3.960 1.744 1.357 0.258 -0.712 1.502 0.448 0.397 1.405 -0.855 -0.132 0.175 0.519 -1.424 2.717 2.010 -0.921 0.909 2.040 0.978 -0.818 -0.885 -0.330. TF mode Mean intensity (volume weighted) -0.108 -0.004 0.662 0.132 1.675 1.922 1.381 2.889 4.073 2.931 1.618 0.586 -0.041 1.982 -0.527 0.916 1.383 1.507 4.485 0.308 -0.140 1.089 -0.537 -0.111 0.005 -0.868 0.043 -0.253 1.570 0.453 0.725 0.060 -0.343 -1.074 -1.421 -0.323 0.556 -0.499 -0.005 0.741 6.225 1.617 1.862 1.800 3.265 1.281.
(243) ! !. TF mode Mean intensity (mean int. weighted) -0.402 0.000 0.736 0.201 1.827 1.821 1.160 3.306 4.083 3.082 1.776 0.964 0.254 2.180 -0.417 0.923 1.714 1.839 5.314 0.661 -0.189 1.313 -0.403 -0.295 0.370 -0.886 0.488 -0.228 2.124 0.586 0.547 0.006 -0.583 -0.997 -1.403 -0.713 1.016 -0.352 -0.087 0.804 5.924 1.802 2.214 2.096 3.544 1.506. TF mode Mean intensity 1.917 0.000 0.501 0.108 1.132 0.898 1.505 0.835 0.201 0.792 1.903 -0.022 -0.728 0.300 -1.563 0.180 0.794 -0.227 0.896 -0.016 -0.028 0.972 -1.256 -0.215 -1.151 -0.612 -0.978 -0.671 -1.963 0.015 0.661 -1.279 -1.235 -1.040 -1.291 0.405 -0.308 -0.062 -0.225 -0.138 0.778 1.424 0.059 -0.023 0.983 1.150.
(244) 0.113 0.618 -0.389 1.317 -0.632 1.389 0.164 0.824 0.626 0.948 -1.597 0.875 -0.184 1.864 0.319 0.942 1.299 -0.216 0.175 1.237 0.920 0.082 -1.714 0.122 -0.529 -1.438 0.425 -0.012 0.183 -0.081 -1.467 0.371 0.079 0.153 0.671 1.009 -0.133 -0.733 0.201 -1.096 -0.026 0.189 -0.332 -0.803 0.283 -0.886 -0.856 -1.782 -0.069 -1.977 -1.095 -2.810 -1.571. 1.064 -0.624 1.873 0.153 -0.478 -0.411 -1.037 -0.453 -0.500 0.234 7.205 0.222 -0.298 -1.326 -0.028 -1.406 -0.205 -1.203 -0.013 0.489 -0.580 -0.659 -2.679 -0.645 -0.605 -1.532 -2.059 0.123 -0.948 0.514 2.072 0.288 -1.153 -0.950 0.392 -0.263 0.128 2.153 1.381 1.257 -0.353 -0.348 0.027 0.389 0.315 3.159 1.740 2.846 2.098 1.773 -0.825 1.180 -0.484. -1.007 1.250 1.697 0.801 -0.599 -0.364 -0.908 -0.532 -0.502 0.212 6.441 0.728 -0.180 -0.597 0.409 -1.554 -0.176 -1.240 0.513 0.507 -0.868 -1.054 -2.687 -1.154 -1.115 -1.733 -1.901 -0.189 -1.442 0.393 1.527 -0.039 -1.121 -1.787 0.116 -0.848 0.050 1.617 0.851 0.528 -1.542 -1.020 -1.196 -0.995 -0.134 2.896 1.211 1.278 1.389 -0.777 -1.783 -0.490 -0.709. . -0.450 0.747 1.280 0.322 -0.185 -1.145 -0.766 -0.537 -1.296 -0.118 6.067 -0.146 0.433 -1.473 0.097 -2.119 -0.580 -0.306 0.392 -0.147 -1.326 -0.809 -0.793 -1.348 -0.097 -0.513 -2.301 -0.436 -1.378 0.289 2.252 -0.184 -0.747 -1.217 -0.036 -1.645 0.166 0.387 1.210 1.822 -1.672 -1.023 -0.679 0.250 -0.169 4.197 1.992 2.792 1.340 1.123 -0.062 0.734 1.191.
(245) " !. 1.228 1.246 1.830 1.237 -1.019 0.926 -0.883 0.273 0.594 1.291 0.158 1.670 -0.853 1.573 0.403 0.693 1.034 -1.258 0.433 1.835 0.312 -0.676 -2.581 -0.574 -1.408 -1.900 0.753 -0.121 -0.450 0.189 -1.363 0.171 -1.089 -0.879 0.335 0.877 -0.258 0.594 -0.474 -1.192 0.499 -0.960 -0.635 -1.326 0.177 -0.927 -0.101 -2.053 0.180 -2.401 -2.363 -2.524 -1.956. 1.299 1.269 1.794 1.448 -1.246 0.913 -0.918 0.155 0.582 1.315 -0.415 1.746 -0.800 1.912 0.443 0.645 0.978 -1.373 0.519 2.062 0.375 -0.730 -2.705 -0.614 -1.572 -1.997 0.857 -0.236 -0.499 0.226 -1.528 0.124 -1.132 -0.961 0.351 0.924 -0.314 -0.092 -0.561 -1.290 0.591 -1.004 -0.897 -1.363 0.132 -0.944 -0.007 -2.248 0.119 -2.655 -2.489 -2.694 -2.060. 1.195 1.697 0.744 1.362 -1.482 1.054 -1.070 0.096 0.792 1.140 -1.385 1.570 -0.969 2.105 0.155 0.722 1.030 -1.729 0.288 2.245 0.466 -0.909 -3.035 -0.812 -1.693 -2.274 0.701 -0.263 -0.512 0.307 -1.826 0.094 -1.273 -0.948 0.451 1.344 -0.355 -0.470 -0.411 -1.341 0.609 -0.989 -0.948 -1.451 0.028 -1.047 -0.122 -2.640 0.353 -3.001 -2.693 -3.144 -2.278.
(246) -2.285 -1.502 -2.222 0.669 1.041 1.140 0.841 -0.876 0.645 1.393 0.426 -1.315 -0.768 0.235 0.518 0.418 -1.471 0.136 -0.215 1.214 -0.375 -1.083 -1.367 -0.187 -1.467 -0.057 -2.773 -0.893 -0.597 -0.508 -0.605 0.690 -0.349 -0.765 0.058 -0.622 -0.596 -0.264 -0.296 -1.183 -1.434 -1.105 -0.212 -1.768 -0.167 -0.501 -0.673 -0.460 -1.520 -1.258 -0.131 -4.968 -0.718. 4.850 1.447 1.878 0.111 0.172 0.580 -0.291 -1.179 -1.057 -1.333 -1.013 5.146 0.215 -0.693 -1.331 -0.606 -1.600 -0.211 -1.217 -0.697 -1.181 1.261 -1.173 -1.177 -1.450 -0.462 -1.472 -1.346 -1.241 -1.471 0.555 -1.095 -2.462 -0.676 -1.094 -0.266 -0.640 -0.195 -0.627 1.105 -0.210 0.207 -1.341 -1.383 -0.200 1.779 0.900 -0.344 -0.183 -1.161 1.486 -4.763 -0.204. 4.695 1.216 1.071 0.068 -0.102 0.753 -0.555 -1.647 -1.138 -1.515 -1.075 2.793 -0.210 -0.496 -1.360 -0.711 -2.184 -0.130 -1.418 -0.345 -0.975 0.591 -1.828 -1.419 -1.744 -1.086 -3.176 -1.728 -1.832 -1.779 1.027 -1.321 -3.442 -1.515 -1.508 -0.726 -1.322 -1.192 -1.116 0.695 -0.897 -0.484 -1.679 -1.909 -0.619 0.976 0.436 -0.003 -1.186 -1.856 0.848 -5.572 -0.883. . 7.574 3.108 3.513 -0.086 -0.801 0.592 -0.469 -0.166 -0.735 -2.177 -0.513 1.661 0.900 -0.019 -1.007 -0.574 -0.585 -0.162 -0.560 -1.029 -0.581 0.804 -0.110 -1.059 0.161 -0.738 -1.670 -0.341 -0.824 -1.261 0.634 -1.838 -3.113 -0.147 -1.584 -0.573 -0.474 -0.347 -0.405 2.183 0.301 1.158 -0.754 -0.417 0.398 1.561 1.979 0.107 1.008 -0.340 1.387 -2.100 0.358.
(247) # !. -1.764 -1.410 -1.753 -0.158 1.329 1.403 0.787 -2.141 -0.022 1.280 -0.319 0.688 -1.330 -0.280 0.024 -0.283 -1.820 0.236 -0.741 1.106 -0.757 -0.437 -1.885 -0.966 -2.305 -0.891 -2.143 -1.868 -1.801 -1.265 0.720 0.383 -1.124 -1.281 -0.688 -0.744 -1.032 -1.013 -1.257 -0.826 -1.495 -2.050 -1.912 -2.163 -1.315 -0.791 -1.119 0.767 -2.068 -2.044 -1.053 -3.570 -1.647. -1.975 -1.472 -1.819 -0.327 1.375 1.337 0.503 -2.159 0.295 1.226 -0.255 -1.231 -1.461 -0.409 0.160 -0.353 -1.942 0.272 -0.710 1.051 -0.712 -0.685 -1.947 -1.019 -2.377 -1.028 -2.557 -1.934 -1.915 -1.332 0.606 0.300 -1.249 -1.220 -0.754 -0.853 -1.094 -0.964 -1.359 -0.930 -1.528 -2.219 -2.073 -2.232 -1.357 -0.899 -1.174 0.473 -2.138 -2.085 -1.205 -3.746 -1.728. -2.283 -1.695 -2.093 -0.488 1.590 1.327 0.416 -2.430 0.056 1.514 -0.410 -2.039 -1.643 -0.468 -0.146 -0.516 -2.319 0.048 -0.913 1.147 -0.967 -1.037 -2.169 -1.129 -2.701 -1.226 -3.118 -2.157 -2.097 -1.496 0.507 0.259 -1.459 -1.420 -0.766 -0.958 -1.258 -1.027 -1.409 -1.157 -1.600 -2.473 -2.196 -2.497 -1.378 -1.114 -1.147 0.442 -2.408 -2.199 -1.264 -4.461 -1.812.
(248) -2.078 -0.671 -1.136 -1.379 -0.859 -0.891 -0.239 0.729 0.873 -1.022 1.575 -0.450 -0.072 -2.114 0.876 -0.686 -0.570 -0.608 0.025 0.188 -1.123 0.965 0.251 -0.152 0.352 -1.436 -1.186 1.385 0.218 -1.603 0.438 -0.766 0.552 -0.466 0.073 -1.436 -0.118 0.539 -0.432 -1.365 -0.419 -2.009 -0.414 -1.268 -1.735 -0.517 -2.107 0.511 -0.563 -0.718 -0.093 -1.623 -1.855. -1.395 -0.138 -0.145 0.460 -1.619 0.055 0.035 -0.513 -0.372 -1.594 -0.911 -0.303 -0.861 -1.382 -0.749 -0.840 -1.159 -1.456 -0.886 -1.753 -0.084 -0.103 -1.391 -1.380 0.056 -0.791 -1.718 0.115 -1.160 -0.871 -0.819 0.029 0.277 -1.047 -0.981 -0.582 -1.388 -1.027 0.136 0.612 1.321 1.583 1.168 0.264 -0.573 -0.949 4.090 0.469 0.583 2.101 -1.221 2.246 -1.834. -1.967 -0.369 -0.811 -0.105 -1.821 -0.170 0.358 -0.201 -0.735 -1.604 -0.921 -0.634 -0.626 -1.834 -1.119 -1.373 -0.556 -1.292 -0.872 -2.054 -0.164 0.112 -1.534 -1.831 -0.571 -1.094 -1.763 -0.742 -1.793 -1.305 -1.274 0.012 -0.550 -1.721 -1.318 -1.241 -1.807 -1.543 -0.124 0.311 0.772 1.399 0.494 -0.320 -1.047 -1.253 2.530 -0.086 0.206 1.650 -1.695 0.769 -1.859. . 0.118 0.899 0.212 1.621 -0.246 0.672 0.147 -0.306 -1.107 0.326 -1.527 0.437 0.250 0.633 -1.653 -0.226 0.016 -0.399 -0.484 -1.566 1.324 -0.455 -1.472 -1.199 -1.333 0.188 -0.883 -1.728 -1.490 -0.144 -1.117 1.121 -0.786 -1.277 -1.033 0.153 -1.263 -1.913 0.829 2.069 1.473 4.264 1.122 1.034 0.541 -1.075 3.120 -0.062 1.787 3.287 -1.595 4.104 -0.590.
(249) $ !. -2.537 -1.590 -0.944 -1.497 -1.399 -1.264 0.981 -0.141 0.842 -1.817 2.435 -0.791 -1.080 -2.578 0.800 -1.327 -1.235 -1.410 -0.781 -0.788 -1.507 1.067 0.000 -0.232 0.821 -2.067 -1.666 3.483 -0.986 -1.339 -0.177 -0.766 0.762 -0.688 -0.371 -2.047 -1.004 0.023 -1.390 -1.762 -0.196 -1.918 -0.907 -1.682 -1.941 -0.920 -1.104 0.738 -1.252 -0.820 -0.714 -2.053 -1.851. -2.603 -1.577 -0.898 -1.539 -1.364 -1.343 0.897 -0.311 0.844 -1.834 2.644 -0.923 -1.204 -2.726 0.809 -1.473 -1.333 -1.529 -0.788 -0.760 -1.554 1.318 0.099 -0.363 0.968 -2.161 -1.683 3.582 -1.027 -1.382 -0.151 -0.736 0.834 -0.630 -0.292 -2.133 -1.023 0.005 -1.553 -1.891 -0.216 -1.979 -0.910 -1.839 -2.056 -0.955 -1.750 0.961 -1.343 -1.125 -0.767 -2.140 -1.906. -2.946 -1.707 -0.914 -1.654 -1.594 -1.521 0.739 -0.413 0.988 -2.067 2.590 -1.264 -1.375 -3.084 0.875 -1.761 -1.553 -1.746 -0.870 -0.913 -1.649 1.403 0.098 -0.381 0.979 -2.426 -1.913 3.881 -1.128 -1.540 0.012 -0.877 0.871 -0.808 -0.382 -2.348 -1.113 0.128 -1.642 -2.187 -0.460 -2.236 -0.805 -1.861 -2.315 -1.020 -2.281 0.948 -1.545 -1.369 -0.733 -2.374 -2.045.
(250) -1.575 -0.854 0.200 -1.008 -1.565 0.493 0.863 -1.292 0.506 1.706 1.248 0.424 0.989 1.288 -0.165 0.805 0.926 -0.191 1.008 -0.313 1.106 -1.029 -0.460 -0.463 -0.051 0.006 1.331 0.188 -0.028 0.143 0.688 -0.625 -0.875 -0.017 0.432 -1.145 0.302 -0.955 -0.558 -0.488 -0.709 -0.252 -1.221 -1.692 -1.032 -1.212 -1.954 0.657 -0.023 -0.921 -0.325 0.465 -1.356. -0.345 -0.420 -0.991 -0.055 0.353 -0.742 0.201 -0.640 -1.120 -0.617 -1.271 -0.055 -0.557 -0.421 -0.422 -0.518 -0.650 -1.544 -0.325 -1.604 -0.430 0.039 0.198 -1.273 -1.549 -1.698 -1.646 -0.371 -1.720 0.133 0.385 -1.123 0.067 0.463 -1.320 2.154 -1.031 -1.239 0.663 -0.541 0.301 1.209 1.092 2.180 1.071 -0.223 -0.165 -0.317 0.038 0.196 -0.620 -0.759 1.030. -1.295 -1.488 -1.380 -0.976 0.068 -0.653 0.737 -1.233 -1.146 -0.289 -1.366 -0.066 0.062 -0.650 -0.488 -0.312 -0.729 -2.005 0.044 -1.566 0.300 0.409 0.403 -1.703 -1.981 -2.220 -1.863 -1.253 -2.133 -0.262 0.375 -1.295 -0.133 -0.132 -1.832 0.886 -1.324 -1.822 -0.014 -0.963 -0.572 0.610 0.838 1.639 -0.107 -1.083 -1.371 -0.272 -0.420 -0.065 -0.968 -1.408 0.188. . 0.884 -0.629 -0.995 -0.042 2.194 -0.825 0.063 0.984 -1.295 -1.352 -1.551 -0.407 -0.323 -1.089 -0.281 -0.556 -0.848 -1.721 -0.565 -1.181 -0.701 1.027 -0.232 -0.516 -1.603 -1.433 -2.671 -1.236 -1.496 0.318 0.022 -0.662 0.667 -0.075 -1.797 -0.033 -0.947 -0.935 1.393 0.058 0.329 1.211 2.668 3.413 1.580 0.951 0.732 -1.031 1.153 0.815 -0.758 -1.134 2.716.
(251) % !. -2.117 -1.916 -0.989 -1.340 -1.853 0.316 1.302 -2.100 0.145 1.796 1.116 0.625 0.976 1.538 0.467 1.700 0.366 -0.956 1.173 -0.582 3.337 -0.687 1.149 -1.259 -0.662 -1.373 2.879 -0.225 -1.494 -0.242 1.394 -1.214 -0.758 0.977 -0.359 -0.656 -0.283 -1.853 -0.989 -1.553 -1.757 0.084 -0.986 -1.272 -1.325 -2.104 -2.382 1.204 -1.015 -0.812 -0.909 0.434 -2.079. -2.140 -2.033 -1.069 -1.538 -1.906 0.222 1.311 -2.168 0.230 1.835 1.289 0.673 1.194 1.871 0.457 2.206 0.265 -0.970 1.262 -0.609 3.692 -0.708 1.146 -1.308 -0.682 -1.501 3.086 -0.302 -1.566 -0.250 1.704 -1.250 -0.765 0.886 -0.396 -1.392 -0.286 -1.986 -1.071 -1.497 -2.062 0.254 -0.989 -1.223 -1.311 -2.218 -2.420 1.279 -1.094 -0.812 -1.024 0.393 -2.119. -2.403 -2.319 -1.064 -1.723 -2.120 0.230 1.172 -2.368 0.350 2.126 1.440 0.709 1.092 1.922 0.360 1.806 0.234 -1.097 1.226 -0.965 3.667 -0.967 1.251 -1.342 -0.713 -1.591 3.534 -0.279 -1.727 -0.191 1.778 -1.314 -0.743 0.780 -0.389 -1.821 -0.440 -2.126 -1.143 -1.719 -2.233 0.156 -1.098 -1.407 -1.374 -2.432 -2.668 1.345 -1.026 -0.777 -1.111 0.546 -2.337.
(252) -0.210 -1.355 -0.798 -0.345 -1.560 -0.925 -1.293 0.549 -2.234 -0.279 0.961 -0.223 -0.133 -1.294 -0.608 -2.751 1.587 -2.041 -0.904 0.264 -1.379 -0.663 0.149 -1.782 0.277 0.415 0.810 -0.171 0.261 0.629 1.348 0.128 0.044 -0.109 0.233 -0.792 -1.687 0.104 0.005 -0.730 1.262 0.703 0.121 -0.781 1.014 -0.845 0.236 -1.251 -2.127 -1.096 -0.161 -0.013 -0.679. -0.966 -1.561 -1.463 -0.702 0.842 0.459 0.184 -0.379 -2.261 -0.403 -2.174 -1.709 -1.830 -1.358 0.347 -1.221 -2.134 -0.488 -1.212 -3.656 -1.819 -1.967 -1.161 -0.773 -1.180 0.133 -3.374 -0.881 -3.550 -2.796 -1.472 -1.123 -0.923 -0.517 -0.385 1.588 -1.813 -1.399 -0.586 -0.874 -2.427 -1.613 -0.180 1.590 0.425 -0.187 -0.261 0.867 1.653 -2.635 -2.225 -1.266 -0.970. -1.590 -1.962 -1.819 -1.137 -0.116 0.329 1.822 -2.545 -3.521 -0.282 -1.487 -0.854 -1.866 0.668 0.010 -1.368 -1.524 -0.710 -0.550 -1.334 -1.906 -1.455 -0.271 -0.012 -1.835 0.626 -3.655 -1.402 -3.974 -2.852 -1.355 -1.132 0.753 -0.480 -0.947 0.552 -1.241 1.194 -0.491 -2.168 -1.556 -0.924 0.313 1.046 0.466 -0.218 -0.291 1.264 1.159 -1.752 -2.685 -0.902 -0.620. . -1.382 -0.217 -0.372 -0.919 1.344 1.190 2.130 -1.324 -0.615 -0.238 -2.172 -0.605 -1.331 0.787 1.484 -0.439 -1.221 0.315 -0.241 -1.538 -0.945 -0.920 -0.360 -0.595 -1.454 -0.359 -2.455 -0.966 -2.622 -1.830 -1.060 -1.372 0.210 -0.092 -0.130 0.434 0.181 0.807 -0.433 -0.972 -1.733 -1.351 -0.578 0.838 0.328 0.269 -0.414 1.143 1.252 -1.608 -1.328 -0.234 -0.585.
(253) !. -1.170 -2.478 -1.681 -0.559 -1.637 -0.942 2.054 1.719 1.833 0.687 1.893 3.171 1.685 3.345 1.111 1.061 1.612 0.969 1.814 0.615 1.327 2.880 2.472 2.138 0.855 1.033 1.011 0.099 -0.588 0.331 -0.876 -0.035 1.159 -0.765 -0.313 1.164 -0.086 1.008 -0.219 -0.791 1.591 0.159 1.746 2.628 2.709 -0.629 1.131 -0.815 0.357 0.169 0.741 -0.392 -0.382. -1.323 -2.575 -1.717 -0.594 -1.756 -0.975 2.661 2.050 3.134 1.048 1.867 3.399 1.860 3.979 1.700 1.706 1.828 1.360 2.202 1.025 1.640 3.322 2.259 2.507 1.154 0.665 1.373 0.134 -0.276 0.625 -0.739 0.729 1.288 -0.604 -0.741 0.979 0.202 1.056 0.004 -0.809 1.354 0.129 1.759 2.730 2.652 -0.516 1.202 -0.909 0.450 0.403 0.700 -0.472 -0.545. -1.459 -2.854 -1.862 -0.514 -1.992 -0.960 0.122 0.111 -0.796 1.294 0.752 0.316 0.544 1.054 -0.987 -0.947 2.250 -0.803 0.357 0.687 1.071 1.086 1.046 0.272 1.173 1.436 0.584 -0.637 1.182 -0.207 1.363 0.229 0.998 0.807 -0.091 0.381 -0.779 1.159 -0.117 -0.928 0.906 0.956 0.360 0.930 1.400 -0.162 0.330 -0.265 -0.700 -0.715 -0.455 -0.303 0.242.
(254) -0.892 -0.693 0.622 -1.943 -2.010 0.359 -1.948 -0.916 -1.818 -0.456 -1.000. 0.825 -0.948 -1.070 1.085 -1.464 -0.763 2.034 2.605 -2.255 -1.780 -2.407. 0.147 -0.206 -0.855 -0.216 -2.009 -0.630 1.112 2.384 -2.333 -1.176 -1.546. . 0.235 0.623 -1.441 0.992 -0.088 -1.026 1.234 1.322 -1.807 -0.507 -0.371.
(255) !. 0.414 1.418 1.420 -1.292 0.103 -0.280 0.733 -0.678 1.465 0.613 -0.116. 0.432 1.488 1.426 -1.443 0.180 -0.241 0.725 -0.915 1.670 0.831 0.103. -0.156 -0.428 0.701 -1.492 -1.033 -0.185 -0.287 0.077 -0.302 -0.440 -0.580.
(256) TF mode Integral intensity (volume weighted) 1.782 0.476 0.823 0.426 0.564 0.966 1.062 0.912 1.339 1.919 0.983 -1.192 -0.511 0.550 -1.125 0.250 0.714 0.989 2.920 -0.310 -0.458 0.866 -1.375 -0.312 -1.029 -0.994 -0.885 -0.017 2.465 -0.382 -0.220 -0.632 -0.797 -0.197 -1.093 0.153 -0.329 -0.851 -0.392 1.018 2.948 1.459 -0.178 1.146 0.708 0.239. TF mode Integral intensity (mean int. weighted) 1.460 0.248 0.638 0.366 1.022 1.155 1.204 1.912 1.706 2.158 1.437 -0.759 -0.804 1.965 -1.150 0.518 1.055 0.870 4.640 -0.053 -0.611 0.860 -1.365 -0.116 -1.394 -0.970 -1.131 -0.590 3.092 0.194 -0.229 -0.779 -1.085 -0.663 -1.420 -0.044 -0.336 -0.971 -0.501 1.256 3.643 2.245 0.152 1.071 1.495 0.964. TF mode Integral intensity 2.096 -0.283 0.363 -0.091 1.188 0.892 1.168 0.776 0.132 0.913 1.818 -0.130 -0.752 0.347 -1.645 -0.160 0.471 -0.488 0.956 -0.330 -0.308 1.202 -1.262 0.082 -1.133 -0.609 -1.104 -0.867 -1.875 -0.005 0.525 -1.320 -1.542 -1.182 -1.467 0.216 -0.442 0.111 -0.198 -0.440 0.951 1.738 0.214 -0.255 0.777 0.994. . TF Dist. to TF Dist. to nuc. (volume nuc. (mean TF Dist. to weighted | int. weighted nuc. (nuc. nuc. size | nuc. size size TF/EGF conormalized) normalized) normalized) localization 1.506 1.232 0.418 -3.661 -0.499 -0.519 -0.467 0.642 -0.057 -0.191 0.306 2.164 0.350 0.608 1.875 0.732 1.027 0.951 1.267 1.311 0.186 0.137 -0.027 -0.100 1.732 1.963 2.318 -0.112 0.357 0.244 0.878 1.026 -0.377 -0.026 0.389 2.986 0.500 0.850 2.715 2.846 0.306 0.571 1.637 -0.388 -0.039 0.213 0.557 1.278 0.299 0.560 0.588 -0.876 -0.604 -0.595 0.213 2.834 0.699 0.863 0.924 -1.968 -0.708 -0.679 -0.136 -0.699 1.383 1.424 1.851 0.803 1.357 1.622 2.274 0.367 0.675 0.875 1.375 0.442 -0.585 -0.660 -0.301 -0.548 0.123 0.099 0.670 -0.026 -0.525 -0.507 -0.425 0.880 2.167 2.513 2.808 -1.584 1.502 1.555 1.578 -1.917 1.150 1.391 4.025 -0.943 0.884 0.887 2.146 -0.256 0.604 0.511 2.415 -1.139 0.710 0.596 0.348 -1.712 -0.827 -0.294 1.474 1.807 1.958 1.896 3.508 -1.065 2.063 2.077 4.029 1.090 1.478 1.450 0.757 -1.020 1.444 1.698 1.586 -2.066 0.001 -0.167 -1.106 -1.720 0.341 0.514 -0.242 -2.527 0.953 0.874 1.796 -0.611 0.593 0.741 0.431 -0.476 0.802 0.960 1.461 -0.277 1.194 1.479 2.821 -1.671 0.265 0.048 -0.546 -1.248 -0.927 -0.574 0.680 5.218 -1.106 -1.216 -0.390 0.927 -0.974 -1.077 -1.291 -0.281 -1.044 -0.996 -0.155 1.129 0.417 0.530 2.582 3.291 0.575 0.570 1.805 1.359.
(257) !.
Documents relatifs
At the bottom of the main OP fracture zone, an HHIG facies is observed down to 2380 m MD (cuttings in Fig. 2 ), and primary biotite and local feldspars are completely replaced by
Al- though trauma survivors and nontrauma clients reported a similar frequency of categories for internal and external change as a result of the extended counselling sessions,
By combining the LeARN pipeline, data from the Plant microRNA database (PMRD) and Hevea EST sequences, we identified 48 conserved miRNA families already characterized in other
Dans ce chapitre consacré à la caractérisation des lms d'animation à partir de l'utili- sation conjointe des informations issues de l'analyse des images et de l'analyse des
High accuracy levels in the condition without time restriction made the data from this condition useless for answering the question whether participants detect a conflict between
To this end, infor- mation on the firm-level adoption for seventeen technology applications has been grouped in four broader categories of technologies, which in turn have
origine moyenne ou supérieure (M azaud , 2009). Quand on considère les raisons qui guident les nouveaux entrants vers l’artisanat et le contexte de leur installation, on constate
The purpose of this investigation was to find an economic yet effective method of extracting high quality samples from test pits and excavations in normally consolidated fine
