Modeling poliovirus infection using human engineered neural tissue enriched with motor neuron derived from embryonic stem cells

Article

Reference

Modeling poliovirus infection using human engineered neural tissue enriched with motor neuron derived from embryonic stem cells

COSSET, Erika, et al.

COSSET, Erika, et al . Modeling poliovirus infection using human engineered neural tissue enriched with motor neuron derived from embryonic stem cells. Frontiers in Cell and Developmental Biology , 2021, vol. 8, p. 593106

DOI : 10.3389/fcell.2020.593106 PMID : 33490061

Available at:

http://archive-ouverte.unige.ch/unige:154319

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A

Neurosphere step 2

1

LDN SB

RI

RA

4 12 26

B C D E

RA/shh

ENT step 27

GDNF/BDNF/

IGF-1/shh CpE

42

LDN SB

5

LDN

19

N2B27 medium alone

Neurosphere step 2

1

LDN SB

RI

EGF/FGF

4 12 26

BDNF/GDNF

ENT step

CpE

42

LDN SB

5

LDN

19

N2B27 medium alone BDNF/GDNF

G F

ChaT

HB-9

ISLET1

mnENT lnENT

ChaT

HB-9

ISLET1

A

EV-71 PV-3

enENT

Da y 7

B

MN

PV-3/HB-9/DAPI Non-infected

NS C PSC

PV-3/Nestin/DAPI

PV-3/Oct4/DAPI

Chat/PV-3/DAPI

Day 5

A

A

Day 3

Not infected

Control

B

Fwd CGGACAAACGGCTCACTCT Rev GGACCCGCATGAATCGACTAT Fwd CGGTGGTGGAACCCTACAAC Rev AGGTGGTGACTCCGCTCAT Fwd GGAAGAGAACCTGGGAAAGG Rev CTTGGTCCTTCTCCACCGTA Fwd AATCATCTTTTGCAGATTCTTCTGT Rev TGTCCAAGACTTTCATCTAAGCA Fwd GGGAAGGAATGGGAATCAGT Rev CCCACAGCCACTCTGGTTAT Fwd CAAGCTCAAGAGCCTCATCC Rev TGGGCTGTGTTGAAATGTGT Fwd AGAAGCCAACGTGACATCCT Rev CAAGGGACTTCTGGATCTCG Fwd TGCACAAGAGCATCCAGAAC Rev CGGATTCTCTGGGAGATTTG Fwd TGCACAAGAGCATCCAGAAC Rev CGGATTCTCTGGGAGATTTG Fwd GCTTTGCCTGCTATGAAAGG Rev TTTCCCCTTTTGTCCTTGTG Fwd GTACCATCAAAGCGACAGCA Rev AGTTCTGCATGGCATCCTCT Fwd CTCACCACACACCCCAGATG Rev AGTTCCAGCCCCACTTGCT Fwd AGCAAAAATGACCCACCAATG Rev GGCCTGGATGGTTCAGGATA Fwd AGTGCCCGAAACCCACACTG Rev ACCACACTCGGACCACATCCT Fwd GATTTGTGGGCCTGAAGAAA Rev TTGGGACTGGTGGAAGAATC Fwd GGAAGAGAACCTGGGAAAGG Rev CTTGGTCCTTCTCCACCGTA Fwd AACGATAACATACCAAGCGTGT Rev GGTCTGCCCGTTCAACATC Fwd CTCCATACACTCCAGTGCCAG Rev GCCCACGTAATCCTGATGGT Fwd GGACAAGCTAGGAGGCAGTG Rev ATGGCGATGTTGAGGTCGTG Fwd CACTGAGCACAGTAGGTCCAC Rev ACACCCAGTTGGCTGTCTTC Fwd GCTGCAGCGCAAAGAACC Rev CGGGGGCTCGGTATTGTTAT Fwd AAAGCTGATGAGGGCAAGAA Rev GGCCCCTGAATAAATTCCAT Fwd GGCCTGACAATTTCATCTTTGG Rev ACCACATCCAGGACCGAATC Fwd AAGAGATCCGCACGCAGTAT Rev AGGTCAAGGACTGCAACTGG Fwd GAAGGTGAAGGTCGGAGTC Rev GAAGATGGTGATGGGATTTC GAPDH

PCR

GFAP ChaT HB9

βIII-Tubulin Nestin

Olig2

MAP2 PAX6 NGN qPCR

Nanog Oct4 NeuN βIII-Tubulin

Gene Nucleotide sequence

Nestin

MX1 MDA5

OAS1 OAS2

ALAS1 EEF1A1

RIG1 IRF9 ISG15 ISG20

Ill_ID Symbol Name logFC AveExpr adj.P.Val

ILMN_2110857 GALR3 galanin receptor 3 4.570944 5.077652 5.9E-10

ILMN_3241021 RNY4 RNA, Ro-associated Y4 3.448942 8.44669 7.47E-05

ILMN_1680453 ITM2C integral membrane protein 2C -1.8124 11.06319 0.000617

ILMN_1762899 EGR1 early growth response 1 1.246274 7.978569 0.026036

ILMN_2213759 DBX2 developing brain homeobox 2 0.644193 4.755297 0.030645

ILMN_1777233 E2F2 E2F transcription factor 2 1.722131 7.027879 0.035936

ILMN_1775997 CHAT choline O-acetyltransferase -0.1943 4.7984 0.957261

ILMN_1763390 ISL1 ISL LIM homeobox 1 -1.3826 7.708546 0.784132

ILMN_1697812 MNX1 motor neuron and pancreas home -0.43562 4.785388 0.670618

ILMN_3240365 MNX1 motor neuron and pancreas home -0.32403 5.62749 0.927921