HAL Id: hal-01512188
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Genetic mapping of broad-spectrum QTLs and
strain-specific major QTL for resistance to Ralstonia
solanacearum in eggplant using GBS
Sylvia Salgon, Cyril Jourda, Christopher Sauvage, Marie-Christine
Brand-Daunay, Emmanuel Wicker, Jacques Dintinger
To cite this version:
Sylvia Salgon, Cyril Jourda, Christopher Sauvage, Marie-Christine Brand-Daunay, Emmanuel Wicker,
et al.. Genetic mapping of broad-spectrum QTLs and strain-specific major QTL for resistance to
Ralstonia solanacearum in eggplant using GBS. 16. Eucarpia Capsicum and Eggplant Meeting, Sep
2016, Kecskemét, Hungary. �hal-01512188�
XVI. EUCARPIA
Capsicum and Eggplant MeetingKECSKEMÉT· HUNGARY·12-14.SEPT.2016
in memoriam
Dr.
Alain Palloix
Edit
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6:
Katalin Ertsey-Peregi
Zsuzsanna
F
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stos
Gabor Palot
à
s
Gabor Csilléry
Genetic mapping of broad-spectrum QTLs and strain-specific
major QTL for resistance t
o
Ralstonia solanacearum
in eggplant using GBS
Sylvia Sa
l
gon
1,2,3,C
yril J
ou
r
d
a
1,Christo
ph
e
r
Sa
u
vage", Marie
-
Christi
n
e
D
aunay",
Emma
nu
e
l
Wicker
l,J
a
c
ques Di
n
tinger'
1
CIRAD,
UM
R
PV
B
MT,
Saint
-P
ierre,
La
R
éunion (F
r
ance)
2
ARMEFLHO
R
, Sa
i
nt
-
Pie
rre, L
a
R
éun
i
o
n
(Fr
an
ce)
3Université
de
l
a
R
éuni
o
n, Sai
nt-D
enis, La Réun
i
on (France)
4
INRA UR
I
052,
Montfavet, France
Abstract
The Solanaceae family includes major agricultural cropscultivated worldwide such astomato, potato,
pepper and eggplant. Production of these crops in tropical and subtropical areas is limited by Ralstonia
solanacearum, the causal agent of the bacterial wilt disease. This pathogen represents a threat for crops
due to itslargehost range, itspersistence in fields andits extensive genetic diversity. The species complex
ofR. solanacearum isdivided into fourphylotypes, which ailare able to wilt eggplant.
Seeking forefficient and stable resistance to bacterial wilt,an intraspecific population of 178eggplant
recombinant inbred lines (RlLs) was evaluated for resistance to R. solanacearum strains belonging to
phylotypes I, rrA, IIB, and III.lnaddition of 162 AFLP and SSR markers, genotyping-by-sequencing
(GBS) ofthe RILs provided 661 SNPs usedtoconstruct adense genetic map anchored tothe physical map
oftomato. Quantitative Trait Loci (QTL) analysis showed the presence ofa major QTL (ERs!) specifically
associated with resistance to phylotype 1 strains on the chromosome 9 ofeggplant. Two broad-spectrum
QTLs were also identified onchromosome 2 and chromosome 5. Although less efficient than ERsl, these
QTLs were found to partially control strains belonging tophylotype I,rrAand III.
Molecular markers linked to QTLs will be very useful for breeding resistance to Ralstonia
solanacearum ineggplant. The anchored map willalsohelp toidentify candidate genes underlying the so
far identified resistance factors.
Keyworâs: Eggplant, Ra/stonia solanacearum, resistance, breeding
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