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Table S1 Ventilator settings, respiratory mechanics and blood gases Before BAL After BAL

VT (ml/kg BW) 11.3  1.0 9.9  1.3*

PEEP (cmH2O) 5.0  0.3 4.9  1.0

PIP (cmH2O) 13.0  1.4 20.9  4.6***

fresp (/min) 34.6  6.0 46.1  8.0***

Crs (ml/cmH2O/kg BW) 1.6  0.3 0.7  0.2***

Rrs (cmH2O/ml/s/kg BW) 0.027  0.004 0.039  0.006***

PaO2 (mmHg) 442  46 63  18***

PaCO2 (mmHg) 48  6 52  9

Data are reported as mean ± standard deviation

BAL bronchoalveolar lavage; VT tidal volume; BW body weight; PEEP positive end-expiratory pressure; PIP peak inspiratory pressure; fresp respiratory rate; Crs respiratory system compliance;

Rrs respiratory system resistance; PaO2 arterial partial pressure of oxygen; PaCO2 arterial partial pressure of carbon dioxide. Significantly different values from before BAL are given as * p<0.05;

*** p<0.001. (The significance of differences between the ventilator settings, respiratory system mechanics and blood gases before and after the sequence of BAL procedures was tested with the paired t test or the Wilcoxon signed rank test as appropriate.)

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