Une jument stérile (Equus caballus) de caryotype, XO

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Une jument stérile (Equus caballus) de caryotype, XO

L. Metenier, Marc Antoine Driancourt, Edmond Cribiu

To cite this version:

L. Metenier, Marc Antoine Driancourt, Edmond Cribiu. Une jument stérile (Equus caballus) de cary-

otype, XO. Genetics Selection Evolution, BioMed Central, 1979, 11 (2), pp.161-163. �hal-02729193�

Note

An XO chromosome constitution in a sterile Mare (Equus caballus)

Liliane METENIER M.A. DRIANCOURT E.P. CRIBIU

(’) Laboratoire de Cytogénétique !7.7V.C.!./.!4.-/.7V.7?.!., Centre vaational de Recherches zootechniques,

783$o Jouy-en-Josas (Fvance) (

2

) Station de Physiologie ^{de la} Repvoduction,

Centre la Recherches de Tours, 7.A!.K.!.,

3738 0

Nouzilly (France)

Summary

One case of 6 3 , ^XO chromosome constitution is described in a phenotypically ^{normal but}

sterile mare with gonadal dysgenesis.

Several general descriptions ^{of the} karyotype ^{of the Domestic Horse} (Equus caballus) ^{have been} reported (RoTH!x!I,ns ^et al., 1959 ; T RUJILLO ^et al., Ig62;

S

ASRKI and M AKINO , 19 6 2 ^{and M AKINO et al.,} I g6 3 ). According ^{to the} idiogram established by ^{D E} GiOVANNi et al. ( I g 7 g), ^the diploid ^{number was} 6q of which 6 pairs

were submetacentric, 7 pairs metacentric, ^{the X} metacentric and the Y acrocentric.

In recent years, chromosome studies of Horse have generally ^{been directed}

toward the association of chromosome alterations with phenotypic ^{defects includ-} ing those of the reproductive system (review ^{of DE G IOV A NNI and} C RIBIU , 197 8).

The best known example is the abnormal XO sex chromosome constitution report-

ed by ^{C H A ND i / EY et al.} ( 1975 ), ^{H U G H ES et al.} ( 1975 ) ^{and T AYL O R and T ROMM E R S-}

CHAUSEN-SMITH (1975).

The present report describes the chromosome constitution of a sterile mare.

A phenotypically normal but apparently ^{sterile female was admitted to the} department ^of Physiology ^{of the} Reproduction ^{of N OUZILLY to be} hemicastrated.

The genital ^tractus appeared ^{normal. As seen by rectal} palpation, ^{the ovary}

was very small, smooth, ^{firm with no} follicules on its surface; histological ^exa-

mination showed that it was completely devoided of follicules and thus fully ^made

of stroma tissu only.

Blood samples ^were obtained from the jugular vein for chromosome analysis.

Leucocytes ^were cultured and harvested according ^to MOORH!AD et al. (ig6o).

C-banding ^{was achieved} by ^a modification of the technique ^{of S UMNER} (r 97 2).

Chromosome preparations ^were observed with ^a I,eitz-Ortholux microscope

and photographs ^{were taken on Kodak microfile film with a} I,eitz-Orthomat ^camera.

The arrangement of the horse chromosomes in karyotype ^{were made} according

to D E G IOVANNI et al. (1979).

All _{the 47} metaphases ^{counted had a} chromosome number of 2 n = 6 3 (fig. 1 ).

The C-banding&dquo;metaphases showed this mare to have anormal autosomal complement

and only ^{one X} chromosome.

The abnormal XO sex chromosome constitution is well known in ^man and it

can caused by ^either paternal ^{or maternal} non-disjunction. A,ffected individuals

are phenotypic ^{females but usually} display infantile sexual development and /or

assortment of somatic abnormalities (Turner’s ^{Syndrome) and are sterile.}

All the XO mares reported by ^{C HANDLEY et al.} ( I g 75 ), ^{H U G H ES et al.} ( 1975 )

and TA YL O R and TROMM!RSCHAUS!N-SMITH ( 1975 ) ^{did not vary from our case.}

They ^were phenotypically ^normal female and had inactive ovaries (or ^not ovary) lacking germ cells.

Our data demonstrate that cytogenetic ^{tests could be a} valuable tool to explain

potential infertility ^{in mares.}

Acknowledgments

We thank Dr. C.P. Porascu (Laboratoire ^de Cytogénétique U.N.C.E.I.A.-I.N.R.A., C.N.- R.Z., Jouy-en-Josas, France) ^for helpful discussions and critical reviews of the manuscript.

Résumé

Une jument ^stérile (Equus caballus) ^de caryotype, ^XO

Une jument ^stérile, phénotypiquement normale mais présentant

dysgénie gonadique

a été trouvée porteuse d’une monosomie pour le chromosome sexuel X.

References

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