PCR-based assays for detecting Xanthomonas axonopodis pv. allii in onion seed
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Investigate the relationship between vegetative growth and reproduction at different scales: the terminal growth unit, the scaffold branch, the whole tree. What is the effect of
CIRAD, UMR Peuplements Végétaux et Bioagresseurs en Milieu Tropical (PVBMT), Saint-Pierre, La Réunion, France a ; INRA, Laboratoire des Interactions Plantes Micro- Organismes
1) ANSES - LSV, unité Ravageurs et Agents Pathogènes Tropicaux (RAPT), 7 chemin de l'Irat, Pole de Protection des Plantes, 97410 SAINT PIERRE, [email protected]. 2) CIRAD,
No amplification product was observed when the multiplex PCR assay was performed for saprophytic strains isolated from onion or for bacteria belonging to other bacterial genera
The collaborative trial consisted in determining the variability of the results obtained by several different competent laboratories using identical samples and in comparing
We successfully detected both bacterial DNA and internal control DNA from plant by performing two successive steps: homogenization of a seed macerate with a stomacher®,
allii from onion seed by Real Time Multiplex PCR.. Bacterial Blight of Onion (BBO), an
Group III contained strains isolated from other aroid genera that tested negative with MAb Xcd108 and were pathogenic to the host from which they were obtained but not to