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Annexin-A5 organized in 2D-network at the plasmalemma eases human trophoblast fusion OPEN

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Academic year: 2021

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Figure 1.  AnxA5 is expressed at the junction between cyto- and syncytiotrophoblasts. (A) Immunostaining  of AnxA5, CK7 and nuclei (TOPRO-3) in human placental biopsies
Figure 2.  Recombinant AnxA5 induces human trophoblast fusion and PS flip. (A) Trophoblasts were  first incubated with recombinant AnxA5 or AnxA5-2Dmut and immunostained for desmoplakin (DSP,  green)
Figure 4.  AnxA5 interactome at plasmalemma of human trophoblast. Proteins purified by sub-cellular  membrane fractionation from cytrophoblasts (CT) or syncytiotrophoblasts (ST) were subjected to a solid phase  binding assay-using AnxA5-CF770 (0.5 μ M; Anx
Table 1.   Identification of AnxA5-binding proteins. Interacted-AnxA5 proteins from trophoblast membrane  fraction were identified by nanoLC-LTQ Orbitrap mass spectrometry analysis of tryptic digests of bands excised  from polyacrylamide gels after SDS-PAG
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