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Environmental DNA, an innovative method for the early detection of aquatic species: The case of the invasive crayfish Cherax quadricarinatus.
Poster · April 2021
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Environmental DNA, an innova1ve method for the early detec1on of aqua1c species:
The case of the invasive crayfish Cherax quadricarinatus.
Thomas Baudry
*1-2-3-4, Quen1n Mauvisseau
5,6, Jean-Pierre Goût
1, Alexandre Arque
2, Carine Delaunay
3, JulieAe Smith-Ravin
4, Michael Sweet
6& Frédéric Grandjean
3*thmsbaudry@gmail.com
1DEAL Direc2on de l’Environnement, de l’Aménagement et du Logement, Schoelcher 97274, Mar2nique
2ODE Office De l’Eau, Fort-de-France, Mar2nique
3Laboratoire Ecologie et Biologie des Interac2ons, UMR CNRS 7267, Université de Poi2ers, France
4Groupe BIOSPHERES, Université des An2lles, Mar2nique, F.W.I.
5Natural History Museum, University of Oslo, Oslo, Norway
6Aqua2c Research Facility, University of Derby, Derby, DE22 1GB, UK
Introduc1on
Lesser An'lles archipelago in the Caribbean is known as abiodiversity hotspot, hos'ng many endemic species.
Introduc'on of a highly invasive species o>en leads to significant threats to this fragile ecosystem.
First essen'al step in their management is theearly detec0onto iden'fy the invaded areas and the non-invaded in order to contain species.
Here wedeveloped, validated, and op0mized a species-specific eDNA-based qPCR detec0onprotocol targe'ng the 16S region of the mitochondrial gene ofCherax quadricarinatus, a freshwater invasive crayfish in Mar'nique.
Method
Valida0onSampling protocol
Water Filtra*on DNA extrac*on qPCR treatment
Results
Assay valida0on:
High species-specific -in silico
- in vitro
- all biomass treatment amplified -in situ
Highly sensi0ve
- Limit of Detec'on = 1.19x10-6 ng.µL-1 - Limit of Quan'fica'on = 1.91x10-5 ng.µL-1
- C. quadricarinatusHigh validated and optimizin Martinique.edeDNA method for - efficiency.Influence of biomass on eDNA detection - water.Persistence of eDNA up to 23 days in warm Þ method for large-scale study.eDNA seems to be more reliable than traditional Þ Adaptable
to all aquatic (marine and freshwater) species !!
The invasion situation in Martinique is a disaster with watersheds being totally invaded …
1- Significa0ve influence of biomass on detec0on efficiency (t = 8.1001, p-value = 9.502e-06)
2- Decrease of eDNA detec0on efficiency over 0me aUer crayfish removal
3- Persistence of eDNA up to 23 days (1 crayfish treatment) and more (28 days for 2 crayfishes treatment)
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