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Environmental DNA, an innovative method for the early detection of aquatic species: The case of the invasive crayﬁsh Cherax quadricarinatus.
Poster · April 2021
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Environmental DNA, an innova1ve method for the early detec1on of aqua1c species:
The case of the invasive crayﬁsh Cherax quadricarinatus.
, Quen1n Mauvisseau5,6
, Jean-Pierre Goût1
, Alexandre Arque2
, Carine Delaunay3
, JulieAe Smith-Ravin4
, Michael Sweet6
& Frédéric Grandjean3
1DEAL Direc2on de l’Environnement, de l’Aménagement et du Logement, Schoelcher 97274, Mar2nique
2ODE Oﬃce De l’Eau, Fort-de-France, Mar2nique
3Laboratoire Ecologie et Biologie des Interac2ons, UMR CNRS 7267, Université de Poi2ers, France
4Groupe BIOSPHERES, Université des An2lles, Mar2nique, F.W.I.
5Natural History Museum, University of Oslo, Oslo, Norway
6Aqua2c Research Facility, University of Derby, Derby, DE22 1GB, UK
Lesser An'lles archipelago in the Caribbean is known as abiodiversity hotspot, hos'ng many endemic species.
Introduc'on of a highly invasive species o>en leads to signiﬁcant threats to this fragile ecosystem.
First essen'al step in their management is theearly detec0onto iden'fy the invaded areas and the non-invaded in order to contain species.
Here wedeveloped, validated, and op0mized a species-speciﬁc eDNA-based qPCR detec0onprotocol targe'ng the 16S region of the mitochondrial gene ofCherax quadricarinatus, a freshwater invasive crayﬁsh in Mar'nique.
Water Filtra*on DNA extrac*on qPCR treatment
High species-speciﬁc -in silico
- in vitro
- all biomass treatment ampliﬁed -in situ
- Limit of Detec'on = 1.19x10-6 ng.µL-1 - Limit of Quan'ﬁca'on = 1.91x10-5 ng.µL-1
- C. quadricarinatusHigh validated and optimizin Martinique.edeDNA method for - efficiency.Influence of biomass on eDNA detection - water.Persistence of eDNA up to 23 days in warm Þ method for large-scale study.eDNA seems to be more reliable than traditional Þ Adaptable
to all aquatic (marine and freshwater) species !!
The invasion situation in Martinique is a disaster with watersheds being totally invaded …
1- Signiﬁca0ve inﬂuence of biomass on detec0on eﬃciency (t = 8.1001, p-value = 9.502e-06)
2- Decrease of eDNA detec0on eﬃciency over 0me aUer crayﬁsh removal
3- Persistence of eDNA up to 23 days (1 crayﬁsh treatment) and more (28 days for 2 crayﬁshes treatment)
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