Quantification of multiple bile acids in uninephrectomized rats using ultra-performance liquid chromatography-tandem mass spectrometry

Electronic supplementary information

Figure S1

Fig. S1. Comparison of signal to noise ratios (SNRs) of several BAs at the lower limit of detection (LOD) in positive and negative ion modes.

Figure 2S 2 x10 -0 0 0 0 0 0 0 0 0 0 0 1

Counts (%) vs. Acquisition Time (min)

11.5 12 12.5 13 13.5 14 14.5 15 15.5 16 16.5 17 17.5 18 18.5 19 19.5 20 - MRM (375.3000 -> 375.3000) 4.d 2 x10 -0 0 0 0 0 0 0 0 0 0 0 1 1

Counts (%) vs. Acquisition Time (min)

11.5 12 12.5 13 13.5 14 14.5 15 15.5 16 16.5 17 17.5 18 18.5 19 19.5 20

+ MRM (359.3000 -> 135.1000) 11BHSD1__C4.d

* 4.0

Fig. S2. Comparison of molecular ion intensity RI /&$LQQHJDWLYHLRQ PRGHĺ;

upper panel) and positive ion mode ĺ ; lower panel) using MRM. The signal to noise ratio (SNR) of the latter was 4.0, thus above the lower limit of detection.



Published in "$QDO\WLFDO0HWKRGV" which should be cited to refer to this work.

Table S1. Extraction recovery of BAs in charcoal-treated serum.

Bile acids Extract recovery (%)

CA 84 ± 12 CDCA 79 ± 20 DCA 67 ± 19 7-oxoDCA 71 ± 23 HDCA 80 ±6 LCA 74 ± 17 7-oxoLCA 76 ± 13 Į-MCA 72 ± 15 ȕ-MCA 70 ± 11 Ȧ-MCA 73 ± 12 UDCA 79 ± 11 G-CA 68 ± 19 G-CDCA 69 ± 17 G-DCA 76 ± 24 G-LCA 75 ± 11 G-7-oxoLCA 76 ± 15 G-UDCA 76 ± 16 T-CA 86 ± 17 T-CDCA 80 ± 17 T-DCA 88 ± 10 T-LCA 82 ± 9 T-7-oxoLCA 77 ± 19 T-ȕ-MCA 81 ± 15 T-UDCA 74 ± 23

Data are presented as the average ± % R.S.D of two levels of QC concentrations (200 nM and 2000 nM), six samples per concentration.