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Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production

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Academic year: 2021

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Figure

Fig 1. Identification of HBc-interacting proteins in the nucleus of dHepaRG cells. (A) Schematic view of HBc purification process
Fig 2. HBc nuclear interactome. (A) Proteins significantly associated to HBc in the presence of Benzonase were analyzed using the Genemania plugin in Cytoscape (3.7.1)
Fig 3. Validation analyses. (A) Relative abundances of the 11”founder” RBPs identified in HBc nuclear complexes submitted or not to Benzonase treatment
Fig 4. Effect of SRSF10 or RBMX KD on HBV replication in PHH. (A) Outline of the experimental protocol: cells were transfected with siRNA targeting SRSF10 or RBMX or control siRNA (siCTL) and then infected with HBV (MOI of 100 vge/cell)
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