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Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells.

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HAL Id: pasteur-00588916

https://hal-riip.archives-ouvertes.fr/pasteur-00588916

Submitted on 27 Apr 2011

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Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular

endothelial cells.

Michael C W Chan, Renee W Y Chan, Wendy C L Yu, Carol C C Ho, W. H.

Chui, C. K. Lo, Kit M Yuen, Y. I. Guan, John M Nicholls, J S Malik Peiris

To cite this version:

Michael C W Chan, Renee W Y Chan, Wendy C L Yu, Carol C C Ho, W. H. Chui, et al.. Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells.. Respiratory Research, BioMed Central, 2009, 10, pp.102. �10.1186/1465-9921-10-102�. �pasteur- 00588916�

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This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon.

Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells

Respiratory Research2009,10:102 doi:10.1186/1465-9921-10-102 Michael CW Chan (mchan@hkucc.hku.hk)

Renee WY Chan (reneewy@hku.hk) Wendy CL Yu (h0894037@hkusua.hku.hk)

Carol CC Ho (chui2ho@hku.hk) W H Chui (winghungchui@yahoo.com)

C K Lo (cheukkinlo@yahoo.com.hk) Kit M Yuen (kmy74@hkucc.hku.hk)

Yi Guan (yguan@hkucc.hku.hk)

John M Nicholls (nicholls@pathology.hku.hk) Js MALIK Peiris (malik@hkucc.hku.hk)

ISSN 1465-9921 Article type Research Submission date 17 June 2009 Acceptance date 30 October 2009

Publication date 30 October 2009

Article URL http://respiratory-research.com/content/10/1/102

This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below).

Articles inRespiratory Researchare listed in PubMed and archived at PubMed Central.

For information about publishing your research inRespiratory Research or any BioMed Central journal, go to

http://respiratory-research.com/info/instructions/

For information about other BioMed Central publications go to

Respiratory Research

© 2009 Chan et al., licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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http://www.biomedcentral.com/

Respiratory Research

© 2009 Chan et al., licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Type I-like alveolar epithelial cell differentiation and polarization

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(11)

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Culture and polarization of lung microvascular endothelial cell

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Viruses

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(12)

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Virus replication analysis

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(13)

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Quantification of cytokine and chemokine mRNA by real-time quantitative RT-PCR

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Quantification of cytokine and chemokine proteins by ELISA

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Lectin histochemistry

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(14)

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Statistical analysis

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(15)

@&1%$31)

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(16)

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(30)

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