Article
Reference
Co-Carriage of Metal and Antibiotic Resistance Genes in Sewage Associated Staphylococci
AMIRSOLEIMANI, Atena, BRION, Gail, FRANCOIS, Patrice
Abstract
Controlling spread of resistance genes from wastewater to aquatic systems requires more knowledge on how resistance genes are acquired and transmitted. Whole genomic sequences from sewage-associated staphylococcus isolates (20 S. aureus, 2 Staphylococcus warneri, and 2 Staphylococcus delphini) were analyzed for the presence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs). Plasmid sequences were identified in each isolate to investigate co-carriage of ARGs and MRGs within. BLASTN analysis showed that 67% of the isolates carried more than one ARG. The carriage of multiple plasmids was observed more in CC5 than CC8 S. aureus strains. Plasmid exchange was observed in all staphylococcus species except the two S. delphini isolates that carried multiple MRGs, no ARGs, and no plasmids. 85% of S. aureus isolates carried the blaZ gene, 76%
co-carried blaZ with cadD and cadX, with 62% of these isolates carrying blaZ, cadD, and cadX on the same plasmid. The co-carriage of ARGs and MRGs in S. warneri isolates, and carriage of MRGs in S. delphini, without plasmids suggests non-conjugative transmission routes for [...]
AMIRSOLEIMANI, Atena, BRION, Gail, FRANCOIS, Patrice. Co-Carriage of Metal and Antibiotic Resistance Genes in Sewage Associated Staphylococci. Genes , 2021, vol. 12, no. 10, p. 1473
DOI : 10.3390/genes12101473 PMID : 34680871
PMCID : PMC8535820
Available at:
http://archive-ouverte.unige.ch/unige:156314
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Table S1: Sediment sampling locations
Sample Location ID Sed-Stream-1 Sed-Stream-2 Sed-Stream-3 Sed-Stream-4
Location
Small stream upstream of Veterans Park
West Hickman Creek (upstream of
Veterans Park)
Veterans Park Creek
West Hickman Creek (downstream
of WWTP*) Latitude 37° 58ʹ 49.92ˮ N 37° 58ʹ 50.22ˮ N 37° 57ʹ 29.08ˮ N 37° 56ʹ 4.61ˮ N Longitude 84° 29ʹ 26.09ˮ W 84° 29ʹ 25.15ˮ W 84° 30ʹ 7.09ˮ W 84° 30ʹ 7.44ˮ W
*Wastewater Treatment Plant
