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Antioxidant, anti-inflammatory and antimicrobial activities of methanolic and aqueous extract of the leaves of L.

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Nouioua and Gaamoune, 2020 Journal of Animal & Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024) Vol.43 (2) : 7407-7414. https://doi.org/10.35759/JAnmPlSci.v43-2.1

Antioxidant, anti-inflammatory and antimicrobial activities of methanolic and

aqueous extract of the leaves of Pistacia lentiscus L.

Nouioua Wafa1* andGaamoune Sofiane2

*1Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural Life and Sciences, University Ferhat Abbas Setif, Algeria, El Bez, Sétif 19000.

2National Institute of Agricultural Research –Setif –Algeria, INRAA, BP 80, route des fermes, Sétif Corresponding author email: nouioua.wafa@yahoo.fr

Key words: Antioxidant activity, mastic, antibacterial activity, anti-inflammatory activity.

Publication date 29/02/2020, http://m.elewa.org/Journals/about-japs/

1 ABSTRACT

In this study, The Montaigne of Boutaleb contains a plant heritage characterized by the presence of several species that possess medicinal properties. The methanolic and aqueous extract of the leaves of Pistacia lentiscus were prepared, the yield of the methanolic extract was 2 .99 g the percentages 37.2% and the yield of the aqueous extract is 0.98 g with percentages 19.6%. The total dosage of polyphenols and flavonoids in the methanol extract was 38.27 ± 6.30 mg EAG/g extract and 9.80 ± 0,160, mg EAG/g extract, respectively. The total dosage of polyphenols and flavonoids in the aqueous extract was 26.76± 2.47 mg EAG/g extract and 4, 15±0.09 mg EAG/g extract, respectively. The effect of aqueous and methanolic extracts of mastic leaves were evaluated using the DPPH test. Both extracts have shown a weak trapping effect towards the free radicals with an IC50 68.27 ± 9.96 μg/ml and with an IC50 72.92 ± 9.43 μg/ml respectively, then weak activity antioxidant .The antibacterial activity of the methanolic and aqueous extract of the leaves of Pistacia lentiscus is significant. The aqueous and methanolic extract of mastic leaves possess a very strong anti-inflammatory activity to protect the membrane of human blood red blood cells.

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