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Improvement of the diphenylamine-based spectrophotometric method for nitrate determination

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Improvement of the diphenylamine-based spectrophotometric

method for nitrate determination

Authors

Omar Aoun, Salem Benamara, Lynda Aberkane, Nassima Amrani, Farid Dahmoune, Khodir Madani

Publication date 2013 Journal Analytical Methods Volume 5 Issue 20 Pages 5830-5835 Publisher

Royal Society of Chemistry

Description

The previously published diphenylamine (DPA)-based method (DPAM) for nitrate determination was investigated for its applicability using model solutions. First, the response surface methodology (RSM) was applied to elucidate whether the independent variables i.e. pH (x1) and initial nitrate content (NC) (x2) of samples influence the relative error (RE = dependent variable) of the method or not. The model found (in coded variables) is of the type: RE = 11.12 + 0.46x1 − 15.61x2 − 1.20x1x2 + 7.44x12 + 7.69x22. So, based on the response surface and iso-response curves related to model solutions, it becomes possible to minimize the RE value (<20%) by adjusting the sample pH to about 4. Based on these first results, some improvements were attempted which allowed us to reach recovery rates of (83.9 ± 9.0)% and (87.3 ± 8.9)% in the case of spiked spinach stalk and dam drinking water, respectively.

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