HAL Id: hal-03122976
https://hal.univ-reims.fr/hal-03122976
Submitted on 27 Jan 2021
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The bacterial glycolipids rhamnolipids trigger Induced Systemic Resistance in Arabidopsis
Matthieu Touchard, Schellenberger R, W. Patricio Luzuriaga-Loaiza, Clement Christophe, Bailieul Fabienne, Marc Ongena, Florence Mazeyrat-Gourbeyre,
Sandrine Dhondt-cordelier, Jérôme Crouzet, Stephan Dorey, et al.
To cite this version:
Matthieu Touchard, Schellenberger R, W. Patricio Luzuriaga-Loaiza, Clement Christophe, Bailieul Fabienne, et al.. The bacterial glycolipids rhamnolipids trigger Induced Systemic Resistance in Ara- bidopsis. Journées SFR Condorcet, Jun 2018, Calais, France. �hal-03122976�
The bacterial glycolipids rhamnolipids trigger Induced Systemic Resistance in Arabidopsis
Matthieu Touchard
1, Romain Schellenberger
1, W. Patricio Luzuriaga Loaiza
1,2, Sandra Villaume
1, Christophe Clément
1, Fabienne Baillieul
1, Marc Ongena
2, Florence Mazeyrat-Gourbeyre
1, Sandrine Dhondt-Cordelier
1, Jérôme Crouzet
1, Stephan Dorey
1and Sylvain Cordelier
11 URVVC - EA 4707, University of Reims Champagne Ardenne, France
2 LBMI laboratory, Gembloux Agro-Bio Tech, Université de Liège, Belgium
Methods
Ce projet est cofinancé par l’Union Européenne. L’Europe s’engage en région Grand Est avec le FEDER
Despite the lack of RLse-triggered ROS production in roots, we found that RLse trigger a systemic immune response in A. thaliana against the necrotrophic fungus Botrytis cinerea. However, we did not observed a similar response against the hemibiotrophic bacteria Pseudomonas syringae pv. tomato DC3000.
A transcriptomic approach will be further performed to compare the response to a RLse treatment on roots and leaves. The identification of differentially expressed genes should help us to better characterize the local and/or systemic resistance against the pathogen.
Conclusion
In their environment, plants are frequently challenged by pathogenic microorganisms. To deal with these pathogens, plants possess an arsenal of defence mechanisms, quickly activated after microorganism perception. This perception step involve Microbe-Associated Molecular Patterns (MAMPs) that are recognized by plant cells resulting in plant innate immunity. Early events following MAMPs perception, including production of reactive oxygen species, are already well-characterized at the foliar level, but there is a lack of information on the mechanisms involved at the roots level. We previously showed in the laboratory that naturals rhamnolipids secretome (RLse), produced by several bacterial species including some Pseudomonas sp. and Burkholderia sp., are highly effective on Arabidopsis thaliana leaves to induce local resistance against phytopathogenic microorganisms.
The aim of this study is to determine the ability of A. thaliana roots to perceive RLse, and if these molecules can induce a systemic resistance against the necrotrophic fungus Botrytis cinerea and against the hemibiotrophic bacteria Pseudomonas syringae pv. tomato DC3000 (Pst DC3000).
Introduction
Elicitation in a 96-well plate with RLse or
Ethanol (control) 5 mm petiole
section
Lower half of the root 4 weeks old hydroponic Arabidopsis
plants
Treatment
Roots elicitation with RLse 48 hours before pathogen
inoculation Botrytis cinerea
Inoculation of leaves with a conidial suspension
(1.105 conidia.mL-1)
Pseudomonas syringae
Spray of leaves with bacterial suspension (~ 5.106 CFU.mL-1)
Botrytis cinerea
48 hours after inoculation Measurment of necrosis area
Pseudomonas syringae 72 hours after inoculation
Counting CFU.mg-1 of fresh material
Production of reactive oxygen species (ROS)
Systemic protection assay against Botrytis cinerea and Pst DC3000
Results
RLse perception stimulate ROS production in leaves but not in roots
RLse perception trigger a systemic immune response against B. cine- rea, but not against Pst DC3000.
Monitoring H2O2 production by chemiluminescence
b
a
0 10 20 30
Contro l
RLse 1 µM
Necrosis area (mm²)
Protection assay against Botrytis cinerea
b ab
4.5 5.0 5.5 6.0
Contrôle
RLmix 10 µM
Log10
(
CFU.mg−1)
Protection assay against Pst DC3000
a
b
0 500 1000 1500
Contro l
RLse 10 µM
∑RLU (Relative Light Unit)
Petiole
a b
0 500 1000 1500
Contro l
RLse 10 µM
∑RLU (Relative Light Unit)
Root
