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A multi-tissue analysis in fetal pigs to identify genes involved in the determinism of maturity and survival at birth

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HAL Id: hal-02749790

https://hal.inrae.fr/hal-02749790

Submitted on 3 Jun 2020

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A multi-tissue analysis in fetal pigs to identify genes

involved in the determinism of maturity and survival at

birth

Laurence Liaubet, Maëva Jégou, Isabelle Le Huërou-Luron, Yannick Lippi,

Sami Yammine, Elena Terenina, Isabelle Louveau, Nathalie Iannuccelli,

Christine Lascor, Yvon Billon, et al.

To cite this version:

Laurence Liaubet, Maëva Jégou, Isabelle Le Huërou-Luron, Yannick Lippi, Sami Yammine, et al.. A multi-tissue analysis in fetal pigs to identify genes involved in the determinism of maturity and survival at birth. 9th Intenational Conference on Pig Reproduction (ICPR), Jun 2013, Olsztyn, Poland. 2013, Programm and Abstract Book. �hal-02749790�

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129

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A mul - ssue analysis in fetal pigs to iden fy

genes involved in the determinism of maturity

and survival at birth

Laurence Liaubet 1, Maëva Jegou 2, Isabelle Le Huërou-Luron 2, Yannick Lippi 3, Sami Yammine 1,

Elena Terenina 1, Isabelle Louveau 4,5, Nathalie Iannuccelli 1, Christine Lascor 1, Yvon Billon 6, Pascal Martin 3,

Hélène Quesnel 4,5, Laurianne Canario 1, Magali SanCristobal 1

1INRA, UMR0444 LGC, F-31326 Castanet-Tolosan, France; 2INRA, UR1341 ADNC, F-35590 Saint Gilles,

France; 3Plateau GeT-Trix, ToxAlim UMR INRA/INP/UPS, F-31027 Toulouse, France; 4INRA, UMR1348

PEGASE, F-35590 Saint Gilles, France; 5Agrocampus Ouest, UMR1348 PEGASE, F-35000 Rennes, France; 6INRA, UE1372 GESY, F-17700 Surgères, France

Selection for prolifi cacy and lean growth rate in swine has been associated with a substantial increase in piglet mortality. Th e fi rst 24–48 hours aft er birth represent the most critical period. A major determinant for early survival is piglet maturity at birth which relies strongly on the process of maturation during the last month of gestation.

Th e objective of the current study was to compare progeny from Large White (LW) and Meishan (MS) pigs which diff er for piglet survival and vitality to identify new markers of maturity. Th e two pure MS and LW sows were inseminated with mixed semen from the 2 breeds, so that 4 fetal genotypes were examined (MSx-MS and LWx(MSx-MS in (MSx-MS sows, LWxLW and (MSx-MSxLW in LW sows). Samples of skeletal muscle (Longissimus dorsi), adrenal glands, subcutaneous adipose tissue, small intestine, liver, and whole blood were collected on fetuses at 90 and 110 days of gestation. For gene expression analysis, a 60K microarray has been developed (Agilent Technology) from the  44K Porcine (V2) Gene Expression Microarray that was completed with 17,073 60-mers targeting genes expressed in the immune system, adipose tissue and muscle. A total of 384 hybridizations were obtained (8 fetuses x 4 genotypes x 2 stages of development x 6 tissues) and analyzed with a linear model and a FDR (False Discovery Rate) adjusted p-value of 1% to identify genes diff erentially expressed in the 4 fetal genotypes.

Between 90 and  110 days of  gestation, from 8,000 (intestine) to 28,000 (muscle) transcripts were dif-ferentially expressed depending on the fetal genotype and 314 transcripts were common to the six tissues. Th e comparison between genotypes at each stage of gestation highlighted the diff erence between the two extreme fetuses (LWxLW versus MSxMS) with a mean of 3,700 and 8,000 transcripts diff erentially detected at 90 and 110 days, respectively. Th e contrast of expression between the two categories of crossbred piglets was more subtle with only a mean of 168 and 335 transcripts diff erentially detected at 90 and 110 days re-spectively; this may reveal the infl uence of the maternal and paternal genomes.

Biological interpretation of genomic data is underway. Th is genetic study will contribute to a better un-derstanding of the molecular mechanisms involved in piglet maturity determinism and to the identifi cation of biomarkers of maturity.

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