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Extent of horizontal gene transfer in evolution of Streptococci of the salivarius group

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Academic year: 2021

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Table 2. One bacterial colony, freshly grown on an agar plate, was suspended in 50 ␮l of TES (10 mM Tris-HCl, 1 mM EDTA, 25% sucrose), and DNA was extracted by lysis in a thermocycler at 95°C for 10 min and at 4°C for 15 min
Table 1 summarizes the allelic profiles of the 27 S. salivarius strains used in this study
TABLE 4. Genetic diversity of clusters at the glcK, ilvC, and pepO loci
FIG. 5. HGT events detected in S. salivarius pepO, ilvC, pyrE, and tkt genes. Regions where there are high and low levels of diversity are indicated by brackets, and the level of diversity is shown for each region
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