Additional file 1. Estimation of the fungal biomass in two parents and 81 DH lines at 2 dpi. A) Percentage of F. graminearum reads in RNA−seq data. B) The mycotoxin DON measured by ELISA. C) F. graminearum GAPDH and D) ß−tubulin RNA levels measured using RT−qPCR.
HC382 HC401 HC373 HC491 HC391 HC520 HC400 HC405 Nyubai HC480 HC455 HC492 HC453 HC376 HC447 HC474 HC392 HC504 HC503 HC462 HC413 HC375 HC440 HC484 HC411 HC500 HC389 HC409 HC443 HC460 HC487 HC417 HC425 HC479 HC388 HC371 HC384 HC421 HC497 HC458 HC406 HC395 HC494 HC467 HC378 HC475 HC496 HC536 HC490 HC423 HC397 HC418 HC478 HC486 HC442 HC399 HC404 HC434 Wuhan HC390 HC521 HC372 HC488 HC439 HC407 HC485 HC374 HC450 HC441 HC512 HC422 HC444 HC437 HC386 HC438 HC498 HC415 HC501 HC454 HC493 HC472 HC385 HC469
%Fg reads
0 2 4 6 8 10 12 14
A)
HC382 HC401 HC373 HC491 HC391 HC520 HC400 HC405 Nyubai HC480 HC455 HC492 HC453 HC376 HC447 HC474 HC392 HC504 HC503 HC462 HC413 HC375 HC440 HC484 HC411 HC500 HC389 HC409 HC443 HC460 HC487 HC417 HC425 HC479 HC388 HC371 HC384 HC421 HC497 HC458 HC406 HC395 HC494 HC467 HC378 HC475 HC496 HC536 HC490 HC423 HC397 HC418 HC478 HC486 HC442 HC399 HC404 HC434 Wuhan HC390 HC521 HC372 HC488 HC439 HC407 HC485 HC374 HC450 HC441 HC512 HC422 HC444 HC437 HC386 HC438 HC498 HC415 HC501 HC454 HC493 HC472 HC385 HC469
DON
0 50 100 150 200 250 300
B)
HC382 HC401 HC373 HC491 HC391 HC520 HC400 HC405 Nyubai HC480 HC455 HC492 HC453 HC376 HC447 HC474 HC392 HC504 HC503 HC462 HC413 HC375 HC440 HC484 HC411 HC500 HC389 HC409 HC443 HC460 HC487 HC417 HC425 HC479 HC388 HC371 HC384 HC421 HC497 HC458 HC406 HC395 HC494 HC467 HC378 HC475 HC496 HC536 HC490 HC423 HC397 HC418 HC478 HC486 HC442 HC399 HC404 HC434 Wuhan HC390 HC521 HC372 HC488 HC439 HC407 HC485 HC374 HC450 HC441 HC512 HC422 HC444 HC437 HC386 HC438 HC498 HC415 HC501 HC454 HC493 HC472 HC385 HC469
Fg−ßTUB
0 10 20 30 40 50
C)
HC382 HC401 HC373 HC491 HC391 HC520 HC400 HC405 Nyubai HC480 HC455 HC492 HC453 HC376 HC447 HC474 HC392 HC504 HC503 HC462 HC413 HC375 HC440 HC484 HC411 HC500 HC389 HC409 HC443 HC460 HC487 HC417 HC425 HC479 HC388 HC371 HC384 HC421 HC497 HC458 HC406 HC395 HC494 HC467 HC378 HC475 HC496 HC536 HC490 HC423 HC397 HC418 HC478 HC486 HC442 HC399 HC404 HC434 Wuhan HC390 HC521 HC372 HC488 HC439 HC407 HC485 HC374 HC450 HC441 HC512 HC422 HC444 HC437 HC386 HC438 HC498 HC415 HC501 HC454 HC493 HC472 HC385 HC469
Fg−GAPDH
0 50 100 150 200
D)
