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β-Adrenergic cAMP Signals Are Predominantly Regulated by Phosphodiesterase Type 4 in Cultured Adult Rat Aortic Smooth Muscle Cells

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Academic year: 2021

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Table 1. Primers pairs used in RT-PCR to quantify mRNA expression.  Target  Accession  number  Primers  Forward 5’→ 3’  Reverse 5’→ 3’  Product  size (Bp)  Annealing  temperature (°C)  PDE1A  NM_030871  GAAGTTTCGCAGCATTGTCC  GCAGGATATGTCAAACCAACC  96  60
Table 2. Effect of PDE inhibition on basal cytosolic cAMP signal in RASMCs.
Table  3.  Effect  of  PDE  inhibition  on  basal  submembrane  cAMP  signal  in  RASMCs

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